Development of a Streptococcus pneumoniae Keratitis Model in MiceMoore III Q.C.a · McCormick C.C.a · Norcross E.W.a · Onwubiko C.a · Sanders M.E.a · Fratkin J.b · McDaniel L.S.a · O’Callaghan R.J.a · Marquart M.E.a
Departments of aMicrobiology and bPathology, University of Mississippi Medical Center, Jackson, Miss., USA
Mary E. Marquart, PhD
Department of Microbiology
University of Mississippi Medical Center, 2500 North State Street
Jackson, MS 39216 (USA)
Tel. +1 601 815 6934, Fax +1 601 984 1708, E-Mail Mmarquart@microbio.umsmed.edu
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Background:Streptococcus pneumoniae is a common cause of bacterial keratitis, and models to examine the ocular pathogenesis of this bacterium would aid in efforts to treat pneumococcal keratitis. The aim of this study was to establish a murine model of pneumococcal keratitis. Methods: The corneas of A/J, BALB/c or C57BL/6 mice were scratched and topically infected with a clinical strain of S. pneumoniae. Slitlamp examination (SLE), enumeration of bacteria in the corneas and histology were performed. Results: Bacteria were recovered from the eyes of A/J mice on postinfection (PI) days 1 [1.96 ± 0.61 log10 colony-forming units (CFU)] and 3 (1.41 ± 0.71 log10 CFU). SLE scores were significantly higher in the infected A/J mice as compared to the BALB/c or C57BL/6 mice on PI day 3 (p < 0.0001) and steadily increased over time, reaching a maximal value of 3.00 ± 0.35 on PI day 10. Histopathology revealed stromal edema and the influx of polymorphonuclear leukocytes on PI days 7 and 10, and corneal disruption on PI day 7. Conclusions:S. pneumoniae keratitis was established in A/J mice, but not BALB/c or C57BL/6 mice.
© 2009 S. Karger AG, Basel
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