Hormone Research in Paediatrics
Original Paper
N-Glycosylated Variants of Growth Hormone in Human Pituitary ExtractsGarcía-Barros M. · Costoya J. · Ríos R. · Arce V. · Devesa J.Departamento de Fisiología, Facultad de Medicina, Santiago de Compostela, España
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Article / Publication Details
Published online: August 11, 2000
Issue release date: 2000
Number of Print Pages: 6
Number of Figures: 4
Number of Tables: 0
ISSN: 1663-2818 (Print)
eISSN: 1663-2826 (Online)
For additional information: https://www.karger.com/HRP
Abstract
This study was designed to investigate the existence, in human pituitary extracts, of growth hormone (GH) variants not encoded by the hGH-N gene. Using anion exchange-fast protein liquid chromatography followed by SDS-PAGE, we isolated several basic forms of pituitary GH. Incubation of these basic forms with endoglycosidase F/N-glycosidase F revealed that two of them (about 34 and 12 kD) were N-glycosylated. In contrast, no changes were found when samples were incubated with the O-linked glycosylation-specific O-glycosidase. Since the GH-N molecule lacks consensus sequences for N-linked glycosylation, our findings suggest that GH genes other than hGH-N are expressed in the human pituitary gland.
© 2000 S. Karger AG, Basel
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References
- Chen LY, Liao YC, Smith DH, Barrera-Saldaña HA, Gelinas RE, Seeburg PH: The human growth hormone locus: Nucleotide sequence, biology and evolution. Genomics 1989;4:479–497.
- Seeburg PH: The human growth hormone gene family: Nucleotide sequences show recent divergence and predict a new polypeptide hormone. DNA 1982;1:239–249.
- Walker WH, Fitzpatrick SL, Barrera-Saldaña HA, Resendez-Perez D, Saunders GF: The human placental lactogen genes: Structure, function, evolution and transcriptional regulation. Endocri Rev 1991;12:316–328.
- Misra-Press A, Cooke NE, Liebhaber SA: Complex alternative splicing partially inactivates the human chorionic somatomammotropin-like (hCS-L) gene. J Biol Chem 1994;269:23220–23229.
-
Baumann G: Growth hormone heterogeneity: Genes, isohormones, variants and binding proteins. Endocr Rev 1991;21:424–449.
- Weigent DA, Baxer JB, Wear WE, Smith LR, Bost KL, Blalock JE: Production of immunoreactive growth hormone by monoclonal leukocytes. FASEB J 1988;2:2812–2818.
- Mol JA, Henzen-Logmans SC, Hageman PH, Misdorp W, Blankenstein MA, Rijnberk A: Expression of the gene encoding growth hormone in the human mammary gland. J Clin Endocrinol Metab 1995;80:3094–3096.
- Costoya JA, Vidal A, García-Barros M, Arce V, Devesa J: Expression of the human growth hormone normal gene in proliferating and differentiated HL-60 cells. Exp Cell Res 1996;228:164–167.
- Frankenne F, Rentier-Delrue F, Scippo ML, Martial J, Hennen G: Expression of the growth hormone variant gene in human placenta. J Clin Endocrinol Metab 1987;64:635–637.
- Ray J, Jones BK, Liebhaber SA, Cooke NE: Glycosylated human growth hormone variant. Endocrinology 1989;125:566–568.
- Hennen G, Frankenne F, Scippo ML, Igout A, Closset J, Pirens G, Gomez F: Placenta growth hormone: Significance relative to growth hormone and lactogen hormone. Reprod Nutr Dev 1988;28:1699–1706.
- Díaz MJ, Dominguez F, Haro LS, Ling N, Devesa J: A 12-kilodalton N-glycosylated growth hormone-related peptide is present in human pituitary extracts. J Clin Endocrinol Metab 1993;77:134–138.
-
Costoya JA, Arce V, Devesa J: Pattern of presentation of the human growth hormone variant (hGH-V) gene in the normal population. J Pediatr Endocrinol Metab 1998;11:591–595.
- Ono M, Takayama Y, Rand-Weaver M, Sakata S, Yasunaga T, Noso T, Kawauchi H: cDNA cloning of somatolactin, a pituitary protein related to growth hormone and prolactin. Proc Natl Acad Sci USA 1990;87:4330–4334.
- Berghman LR, Lens P, Decuypere E, Kuhn ER, Vandesande F: Glycosylated chicken growth hormone. Gen Comp Endocrinol 1987;68:408–414.
- Bollengier F, Velkeniers B, Hooghe-Peters E, Mahler A, Vanhaelst L: Multiple forms of rat prolactin and growth hormone in pituitary cell subpopulations separated using a Percoll gradient system: Disulphide-bridged dimers and glycosylated variants. J Endocrinol 1989;120:201–206.
- Sinha YN, Lewis UJ: A lectin binding immunoassay indicates a possible glycosylated growth hormone in the human pituitary gland. Biochem Biophys Res Commun 1986;140:491–497.
- Haro LS, Lewis UJ, García M, Bustamante J, Martínez AO, Ling NC: Glycosylated human growth hormone: A novel 24 kDa hGH-N variant. Biochem Biophys Res Commun 1996;228:549–556.
- Sinha YN, Jacobsen BP: Glycosylated prolactin in the murine pituitary: Detection by a novel assay and alteration of concentrations by physiological and pharmacological stimuli. Biochem Biophys Res Commun 1987;148:505–514.
Article / Publication Details
Published online: August 11, 2000
Issue release date: 2000
Number of Print Pages: 6
Number of Figures: 4
Number of Tables: 0
ISSN: 1663-2818 (Print)
eISSN: 1663-2826 (Online)
For additional information: https://www.karger.com/HRP
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