Original Report: Patient-Oriented, Translational Research
KLOTHO Gene Polymorphisms G-395A and C1818T Are Associated with Low-Density Lipoprotein Cholesterol and Uric Acid in Japanese Hemodialysis PatientsShimoyama Y.a · Taki K.a · Mitsuda Y.b · Tsuruta Y.c · Hamajima N.b · Niwa T.a
Departments of aAdvanced Medicine for Uremia, Nagoya University Graduate School of Medicine, and bPreventive Medicine/Biostatistics and Medical Decision Making, Nagoya University Graduate School of Medicine, Nagoya, c Meiyo Clinic, Toyohashi, Aichi, Japan
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Article / Publication Details
Background/Aims: This study aimed to investigate the association of the KLOTHO gene single nucleotide polymorphisms (SNPs), G-395A and C1818T, with various laboratory data in 219 Japanese hemodialysis (HD) patients. Methods: The genotyping of G-395A in the promoter region and C1818T in exon 4 was performed using polymerase chain reaction with confronting two-pair primers (PCR-CTPP) assay. Results: In HD patients, the allele frequencies of G-395A were 0.847 for the G allele and 0.153 for the A allele and those of the C1818T were 0.829 for the C allele and 0.171 for the T allele. There were no significant differences in allele frequencies of G-395A and those of the C1818T between HD patients and healthy subjects. Multivariate analysis adjusted for age and duration on HD demonstrated that uric acid was significantly high in A allele carriers of G-395A compared with GG genotype in all and female patients. Low-density lipoprotein cholesterol was significantly low in T allele carriers of C1818T compared with CC genotype in all and male patients. Conclusion:KLOTHO gene SNPs G-395A and C1818T are associated with low-density lipoprotein cholesterol and uric acid in HD patients.
© 2009 S. Karger AG, Basel
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