International Archives of Allergy and Immunology

Original Paper

Hemopoietic Growth Factors Regulate the Survival of Human Basophils in vitro

Yamaguchi M.a · Hirai K.a · Morita Y.a · Takaishi T.a · Ohta K.a · Suzuki S.a · Motoyoshi K.b · Kawanami O.c · Ito K.a

Author affiliations

aDepartment of Medicine and Physical Therapy, University of Tokyo School of Medicine, Tokyo; bDivision of Hemopoiesis, Institute of Hematology, Jichi Medical School, Tochigi, and cPathology and Clinical Research Laboratory, Nippon Medical School Second Hospital, Kanagawa, Japan

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Int Arch Allergy Immunol 1992;97:322–329

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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Published online: September 02, 2009
Issue release date: 1992

Number of Print Pages: 8
Number of Figures: 0
Number of Tables: 0

ISSN: 1018-2438 (Print)
eISSN: 1423-0097 (Online)

For additional information: https://www.karger.com/IAA

Abstract

Human basophils were purified from normal peripheral blood, using density gradient followed by negative panning selection. We tested the effects of hemopoietic growth factors on the survival of these basophils in vitro. In the absence of exogenous factors, basophils (purity > 90%) decreased in number rapidly. At day 7 only 11% of the cells remained alive in cultures; < 1% of cells survived at day 14. Interleukin (IL)-3 maintained numbers of viable cells; cell viability was 67% at day 7 and 45% at day 14. Granulocyte-macrophage (GM)- colony-stimulating factor (CSF) exhibited slight effect on the survival; 33% of cells remained at day 7. Other growth factors including granulocyte (G)-CSF macrophage (M)-CSF and IL-4 had no significant effect on the survival of basophils at all. Morphological and functional characterization of cells maintained by IL-3 revealed that they belonged to the basophil lineage. These observations indicate that normal basophils possess functional receptors for IL-3 and GM-CSF and that both factors modulate immediate- and delayed-type hypersensitivity reactions by prolonging the life span of basophils.

© 1992 S. Karger AG, Basel




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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Published online: September 02, 2009
Issue release date: 1992

Number of Print Pages: 8
Number of Figures: 0
Number of Tables: 0

ISSN: 1018-2438 (Print)
eISSN: 1423-0097 (Online)

For additional information: https://www.karger.com/IAA


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