International Archives of Allergy and Immunology

Original Paper

Characterization of Murine Monoclonal Antibodies to Phospholipase A2 and Mellitin from Bee Venom

Müller W.-D. · Fahlbusch B. · Jung K. · Gebhardt M. · Jäger L.

Author affiliations

Department of Clinical Immunology, Friedrich Schiller University Jena, FRG

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Int Arch Allergy Immunol 1993;101:153–158

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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Published online: September 03, 2009
Issue release date: 1993

Number of Print Pages: 6
Number of Figures: 0
Number of Tables: 0

ISSN: 1018-2438 (Print)
eISSN: 1423-0097 (Online)

For additional information: https://www.karger.com/IAA

Abstract

9 murine monoclonal antibodies (MoAbs), 7 to phospholipase A2 (Pla2; Api m I) and two to mellitin (Api m III) have been produced. Specificities were demonstrated using immunoblotting and enzyme immunoassays (ELISA). Antibodies specific for Pla2 were characterized in detail. Epitope mapping of Pla2-specific MoAbs identified three independent binding regions. 5 selected MoAbs bound to one of these sites compete with specific human IgE. Two of MoAbs characterized are suitable tools to quantify Pla2 in a two-site binding assay.

© 1993 S. Karger AG, Basel




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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Published online: September 03, 2009
Issue release date: 1993

Number of Print Pages: 6
Number of Figures: 0
Number of Tables: 0

ISSN: 1018-2438 (Print)
eISSN: 1423-0097 (Online)

For additional information: https://www.karger.com/IAA


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