International Archives of Allergy and Immunology
Original Paper
Effect of CC Chemokines on Mediator Release from Human Skin Mast Cells and BasophilsHartmann K. · Beiglböck F. · Czarnetzki B.M. · Zuberbier T.Virchow Clinics, Humboldt University of Berlin, Germany
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Article / Publication Details
Received: March 20, 1995
Accepted: May 30, 1995
Published online: September 04, 2009
Issue release date: 1995
Number of Print Pages: 7
Number of Figures: 0
Number of Tables: 0
ISSN: 1018-2438 (Print)
eISSN: 1423-0097 (Online)
For additional information: https://www.karger.com/IAA
Abstract
Chemokines are considered important mediators of various inflammatory processes. In human basophils, different CC chemokines are known to stimulate release of histamine and generation of leukotriene (LT)C4. In the present study, we have evaluated the effect of RANTES (regulated upon activation, normal T cell expressed and secreted), monocyte chemotactic protein (MCP)-1 MCP-2, MCP-3, macrophage inflammatory protein (MΙP)-1α and MlP-1β on mast cell activation. Whereas all these CC chemokines caused dose-dependent release of histamine from basophils in mixed human leukocyte suspensions, none of them was able to induce release of histamine as well as tryptase or prostaglandin (PG)D2 from human skin mast cells, nor did priming with these substances enhance IgE-mediated mediator release. In addition, all chemokines failed to promote changes in the cytosolic free calcium level in the human mast cell line HMC-1. These results add further evidence for the differences between human mast cells and basophils regarding cytokine-dependent activation.
© 1995 S. Karger AG, Basel
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Article / Publication Details
Received: March 20, 1995
Accepted: May 30, 1995
Published online: September 04, 2009
Issue release date: 1995
Number of Print Pages: 7
Number of Figures: 0
Number of Tables: 0
ISSN: 1018-2438 (Print)
eISSN: 1423-0097 (Online)
For additional information: https://www.karger.com/IAA
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