International Archives of Allergy and Immunology
Original Paper
Intranasal Instillation of Diesel Exhaust Particulates and Antigen in Mice Modulated Cytokine Productions in Cervical Lymph Node CellsFujimaki H.a · Saneyoshi K.b · Nohara O.b · Shiraishi F.a · Imai T.baNational Institute for Environmental Studies, Onogawa, Tsukuba, Ibaraki, and bJikei University School of Medicine, Nishi-shinbashi, Tokyo, Japan
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Article / Publication Details
Received: March 22, 1995
Accepted: June 13, 1995
Published online: September 04, 2009
Issue release date: 1995
Number of Print Pages: 6
Number of Figures: 0
Number of Tables: 0
ISSN: 1018-2438 (Print)
eISSN: 1423-0097 (Online)
For additional information: https://www.karger.com/IAA
Abstract
To investigate cytokine production stimulated by diesel exhaust particulates (DEP) and antigen through the intranasal route, mice were administered with DEP mixed with ovalbumin (OA) 3 times at an interval of 3 weeks. After the last instillation, cervical lymph node cells (LNC) were cultured in vitro with OA and antigen-presenting cells. The proliferative response to OA in cervical LNC from mice instilled with DEP and OA was noted to have increased significantly compared to mice instilled with OA alone. Interleukin 4 (IL-4) and interferon (IFN)-γ in culture supernatants were measured with ELISA. OA-stimulated IL-4 production in cervical LNC from mice instilled with DEP and OA markedly increased beyond that in the control mice. In contrast, OA-stimulated IFN-γ production in cervical LNC from mice instilled with OA was 3 times that for DEP and OA-instilled mice. OA-specific IgE antibody in sera showed a trend to be increased in mice intranasally instilled with DEP and OA. These results suggest that intranasal instillation of DEP and antigen in mice may modulate in vitro antigen-stimulated cytokine production from cervical LNC with a consequent increase in IgE antibody production.
© 1995 S. Karger AG, Basel
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Article / Publication Details
Received: March 22, 1995
Accepted: June 13, 1995
Published online: September 04, 2009
Issue release date: 1995
Number of Print Pages: 6
Number of Figures: 0
Number of Tables: 0
ISSN: 1018-2438 (Print)
eISSN: 1423-0097 (Online)
For additional information: https://www.karger.com/IAA
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