Comparison of the Effects of Low Intra-Abdominal Pressure and Pentoxifylline on Oxidative Stress during CO2 Pneumoperitoneum in RabbitsNesek-Adam V.a · Vnuk D.d · Rašić Ž.b · Rumenjak V.c · Kos J.d · Krstonijević Z.b
aDepartment of Anesthesiology, Resuscitation and Intensive Care, bUniversity Department of Surgery, and cDepartment of Medical Laboratory Diagnosis, Sveti Duh General Hospital, and dDepartment of Surgery, Orthopedics and Ophthalmology, School of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
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Background: The aim of this study was to investigate the effect of low-pressure pneumoperitoneum and pentoxifylline, a methylxanthine derivative, in the prevention of injury caused by free oxygen radicals generated during CO2 pneumoperitoneum. Methods: Twenty-eight rabbits were allocated randomly to 4 groups. Control group rabbits (group 1) were subjected to anesthesia for 60 min; group 2 and 3 animals were subjected to a CO2 pneumoperitoneum (15 or 7 mm Hg); and group 4 rabbits received 50 mg/kg pentoxifylline, followed by a 15-mm-Hg pneumoperitoneum. Serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), lactate dehydrogenase (LDH), lipid hydroperoxide, glutathione reductase and total antioxidant status were measured. Results: Compared with group 1, a significant increase in lipid hydroperoxide levels at the end of the pneumoperitoneum and 30 min after deflation and a significant decrease in total antioxidant status 24 h after deflation were recorded in group 2. In addition, a significant increase was observed in ALT, AST and LDH levels. These changes were attenuated by low-pressure pneumoperitoneum, whereas pentoxifylline pretreatment appeared to attenuate only transaminase levels. Conclusion: Low-pressure pneumoperitoneum could attenuate ischemia/reperfusion injury induced by CO2 pneumoperitoneum in a rabbit model whereas pentoxifylline pretreatment appeared to attenuate only transaminase levels. Pentoxifylline did not prevent the development of oxidative stress.
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