Neonatology
Original Paper
Influence of Gliadin on Fetal Chick Intestine in Tissue CultureMothes T.a · Mühle W.a · Müller F.a · Hekkens W.T.J.M.baDepartment of Pathobiochemistry, Karl-Marx-University, Leipzig, GDR; bDepartment of Physiology, State University, Leiden, The Netherlands
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Article / Publication Details
Published online: September 24, 2009
Issue release date: 1985
Number of Print Pages: 6
Number of Figures: 0
Number of Tables: 0
ISSN: 1661-7800 (Print)
eISSN: 1661-7819 (Online)
For additional information: https://www.karger.com/NEO
Abstract
The purpose of this study was to develop a model system for detecting biological effects of gliadin which may be related to coeliac disease. The technique applied was tissue culture of chicken duodenum at several stages of fetal development. A normally occuring increase in disaccharidase activities in cultured tissue explants was diminished by the presence of peptic-tryptic digested gliadin or of a tryptic fragment of α-gliadin (α-GT 18,000). The effect of peptic-tryptic digested gliadin was only demonstrable in the early phase of fetal development (days 10–14), and disappeared at day 16. The release of enzymes into the culture medium was decreased by gliadin at the 12th day of fetal development. The results suggest that gliadin inhibits the differentiation processes of the fetal intestine.
© 1985 S. Karger AG, Basel
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Article / Publication Details
Published online: September 24, 2009
Issue release date: 1985
Number of Print Pages: 6
Number of Figures: 0
Number of Tables: 0
ISSN: 1661-7800 (Print)
eISSN: 1661-7819 (Online)
For additional information: https://www.karger.com/NEO
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