Neonatology

Original Paper

Neutrophils from Preterm Neonates and Adults Show Similar Cell Surface Receptor Expression: Analysis Using a Whole Blood Assay

Falconer A.E.b · Carr R.c · Edwards S.W.a

Author affiliations

Department of aBiochemistry and bHaematology, University of Liverpool, and cDepartment of Haematology, St. Thomas’ Hospital, London, UK

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Biol Neonate 1995;67:26–33

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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Published online: September 30, 2009
Issue release date: 1995

Number of Print Pages: 8
Number of Figures: 0
Number of Tables: 0

ISSN: 1661-7800 (Print)
eISSN: 1661-7819 (Online)

For additional information: https://www.karger.com/NEO

Abstract

Previous work has shown that Fcγ RIII expression in isolated neonate neutrophils is defective. We have re-examined this phenomenon in view of the facts that (1) the receptor is present on mobilisable subcellular stores and (2) commonly used isolation procedures can affect receptor expression in suspensions of isolated neutrophils. Receptor expression was measured by fluorescence-activated cell sorter analysis of neutrophils in unfractionated whole blood. Examination of receptor expression in preterm, term and adult neutrophils indicated small but significantly decreased expression of CR1 and CR3 in preterm neutrophils compared with term neutrophils (p < 0.01). A small decrease in expression was found for Fcγ RI and Fcγ RIII (p < 0.05). No significant difference in expression of Fcγ RII was observed in all groups analysed. These data suggest that isolated preterm neonate neutrophils have greatly decreased expression of Fcγ RIII because of impaired composition or mobilisation of the subcellular stores of this receptor and/or increased lability of the surface receptor which leads to its shedding during purification.

© 1995 S. Karger AG, Basel




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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Published online: September 30, 2009
Issue release date: 1995

Number of Print Pages: 8
Number of Figures: 0
Number of Tables: 0

ISSN: 1661-7800 (Print)
eISSN: 1661-7819 (Online)

For additional information: https://www.karger.com/NEO


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