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Research Article

Free Access

A Large-Scale RNAi Screen Identifies Deaf1 as a Regulator of Innate Immune Responses in Drosophila

Kuttenkeuler D.a · Pelte N.a · Ragab A.a · Gesellchen V.a · Schneider L.a · Blass C.a · Axelsson E.b · Huber W.b · Boutros M.a

Author affiliations

aGerman Cancer Research Center (DKFZ) and University of Heidelberg, Division of Signaling and Functional Genomics, University of Heidelberg, Heidelberg, Germany; bEuropean Molecular Biology Laboratory, European Bioinformatics Institute, Cambridge, UK

Corresponding Author

Prof. Dr. Michael Boutros

German Cancer Research Center (DKFZ) and University of Heidelberg

Im Neuenheimer Feld 580

DE–69120 Heidelberg (Germany)

Tel. +49 6221 42 1951, Fax +49 6221 42 1959, E-Mail m.boutros@dkfz.de

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J Innate Immun 2010;2:181–194

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Innate immune signalling pathways are evolutionarily conserved between invertebrates and vertebrates. The analysis of NF-ĸB signalling in Drosophila has contributed important insights into how organisms respond to infection. Nevertheless, significant gaps remain in our understanding of how the activation of intracellular signalling elicits specific transcriptional programs. Here we report a genome-wide RNA interference survey for transcription factors that are required for Toll-dependent immune responses. In addition to the NF-ĸB homologs Dif, Dorsal and factors of the general transcription machinery, we identified Deformed Epidermal Autoregulatory Factor 1 (Deaf1) to be required for the expression of the Toll target gene Drosomycin in cultured cells and in Drosophila in vivo. We show that Deaf1 is required for the survival of flies after fungal, but not E. coli, infection. We determine that Deaf1 acts downstream of the NF-ĸB factors Dorsal and Dif. These results indicate that Deaf1 is an important contributor to innate immune responses in vivo.

© 2009 S. Karger AG, Basel


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Article / Publication Details

First-Page Preview
Abstract of Research Article

Received: May 08, 2009
Accepted: August 10, 2009
Published online: October 13, 2009
Issue release date: February 2010

Number of Print Pages: 14
Number of Figures: 5
Number of Tables: 0

ISSN: 1662-811X (Print)
eISSN: 1662-8128 (Online)

For additional information: https://www.karger.com/JIN

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