Journal of Vascular Research

Research Paper

Effects of Diabetes and Insulin Treatment of Diabetic Rats on Hyaluronan and Hyaluronectin Production in Injured Aorta

Chajara A.a · Raoudi M.a · Delpech B.c · Courel M.-N.c · Leroy M.d · Basuyau J.P.d · Levesque H.b

Author affiliations

aLaboratoire DIFEMA-MERCI, Faculté de Médecine-Pharmacie, bDépartement de Médecine Interne, CHU Rouen-Bois-Guillaume, cLaboratoire d’oncologie moléculaire et dLaboratoire de biochimie, Centre Henri Becquerel, Rouen, France

Keywords: DiabetesInsulinHyaluronanHyaluronectinAortaInjury

Related Articles for ""
J Vasc Res 1999;36:209–221
https://doi.org/10.1159/000025644

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Article / Publication Details

First-Page Preview
Abstract of Research Paper

Published online: June 18, 1999
Issue release date: May – June

Number of Print Pages: 13
Number of Figures: 4
Number of Tables: 3

ISSN: 1018-1172 (Print)
eISSN: 1423-0135 (Online)

For additional information: https://www.karger.com/JVR

Abstract

The present study was conducted to determine the effect of diabetes with and without insulin treatment on the production of hyaluronen (HA) and distribution of hyaluronectin (HN) in the rat aorta 14 days after injury with a catheter balloon. Injury increased intima-media wet weight (+11%) and DNA content (+37.5%). This increase was slightly enhanced in untreated diabetic rats (+14.7% for wet weight and +48.9% for DNA content) and was significantly greater in diabetic rats treated with insulin (+28.9% for wet weight and +54% for DNA content). HA content increase in the injured aorta of nondiabetic rats (+43.6%) was similar in untreated diabetic (+44.7%) and more pronounced in diabetic rats treated with insulin (+91.3%). HA was markedly expressed in the neointima of nondiabetic rats, particularly near the lumen of the aorta. In untreated diabetic rats, HA was present throughout the neointima and not mainly close to the lumen. HA staining in the neointima of diabetic rats treated with insulin was similar to that in nondiabetic rats. HN was strongly expressed throughout the neointima of all groups. Injury enhanced the production of a high molecular mass HN (>400 kDa); this was not observed either in untreated or in insulin-treated diabetic rats. In conclusion, insulin treatment promoted the proliferative response of aorta to injury and this was associated mainly with increased HA production. This finding suggests that HA, which has been shown to play a crucial role in smooth muscle cell proliferation and migration, may be involved in the promoting effect of insulin treatment on arterial wall reaction to injury.



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Article / Publication Details

First-Page Preview
Abstract of Research Paper

Published online: June 18, 1999
Issue release date: May – June

Number of Print Pages: 13
Number of Figures: 4
Number of Tables: 3

ISSN: 1018-1172 (Print)
eISSN: 1423-0135 (Online)

For additional information: https://www.karger.com/JVR

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1999, Vol.36, No. 3

May – June

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