Complementary Medicine Research
Original Article · Originalarbeit
Comparative Study on the Antiherpetic Activity of Aqueous and Ethanolic Extracts Derived from Cajanus cajan (L.) Millsp.Zu Y.a · Fu Y.a · Wang W.a · Wu N.a · Liu W.a · Kong Y.a · Schiebel H.-M.b · Schwarz G.a · Schnitzler P.c · Reichling J.da Key Laboratory of Forest Plant Ecology, Northeast Forestry University of Harbin, China b Institute of Organic Chemistry, Technical University of Braunschweig, Germany c Department of Infectious Diseases, Virology, University of Heidelberg, Germany d Institute of Pharmacy and Molecular Biotechnology, Department of Biology, University of Heidelberg, Germany
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Article / Publication Details
Published online: January 08, 2009
Issue release date: March 2010
Number of Print Pages: 6
Number of Figures: 0
Number of Tables: 0
ISSN: 2504-2092 (Print)
eISSN: 2504-2106 (Online)
For additional information: https://www.karger.com/CMR
Abstract
Introduction: Aqueous and ethanolic extracts of Cajanus cajan (Fabaceae) were examined in vitro for their antiviral activity against herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2). Materials and Methods: The antiviral activity was determined using a plaque reduction assay. The cytotoxic concentration (CC50) as well as the inhibitory concentration (IC50) of the extracts was determined from dose-response curves. Results: All extracts tested revealed a high antiviral activity against cell-free HSV-1 and HSV-2. The most active one was the Cajanus ethanol extract with IC50 values of 0.022 µg/ml for HSV-1 and 0.1 µg/ml for HSV-2. In order to identify the mode of antiviral action, the extracts were added to the host cells or viruses at different stages of infection. HSV-1 and HSV-2 were considerably inactivated when the viruses were pretreated with the extracts for 1 h prior to cell infection. At maximum non-cytotoxic concentrations of the extracts, plaque formation was significantly reduced by 95–99% for HSV-1 and HSV-2. In a time-dependent assay with cell-free HSV-1 over a period of 2 h, a clearly time-dependent effect was demonstrated whereby the Cajanus ethanol extract revealed a much higher activity than the Cajanus aqueous one. Conclusion: The results obtained indicate that the extracts affect HSV before cell adsorption, but have no effect on the intracellular virus replication. According to our findings, a thera-peutic application of Cajanus ethanolic extracts containing crÈme or ointment as antiviral agent in recurrent HSV in-fection appears to be promising.
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Article / Publication Details
Published online: January 08, 2009
Issue release date: March 2010
Number of Print Pages: 6
Number of Figures: 0
Number of Tables: 0
ISSN: 2504-2092 (Print)
eISSN: 2504-2106 (Online)
For additional information: https://www.karger.com/CMR
Copyright / Drug Dosage / Disclaimer
Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher.Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
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