Fetal Diagnosis and Therapy
Measurement of Adult Hemoglobin in Fetal Blood Samples by High-Performance Liquid Chromatography as Purity Control for the Prenatal Diagnosis of Chromosomal AbnormalitiesDucrocq R.a,e · Tachdjian G.b · Oury J.F.c · Brunie G.a · Fenneteau O.d · Schlegel N.d · Blot P.c · Elion J.a,eServices deaBiochimie Génétique, bCytogénétique, cGynécologie-Obstétrique et dd’Hématologie, et eINSERMU120, Hôpital Robert-Debré, Paris, France
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Article / Publication Details
Received: October 09, 1992
Accepted: March 03, 1993
Published online: November 26, 2009
Issue release date: 1993
Number of Print Pages: 8
Number of Figures: 0
Number of Tables: 0
ISSN: 1015-3837 (Print)
eISSN: 1421-9964 (Online)
For additional information: https://www.karger.com/FDT
Abstract
This study was aimed at testing if measurement of adult hemoglobin (HbA) by ion exchange high-performance liquid chromatography could serve as a purity control of fetal blood samples. We studied 240 samples obtained for karyotyping by cordocentesis under ultrasound guidance. Mean red cell volume (MCV), red cell distribution width (RDW) and HbA were measured on each sample. HbA was determined from 5 μl of blood in 8 min. From 18 to 30 weeks of gestation, HbA values in fetal blood do not vary and are tightly clustered around 5.4% (SD: 1.1%). After 30 weeks, HbA increases as the fetal to adult switch begins. Experimental contaminations of fetal blood by maternal blood show that HbA variations are more pronounced than MCV or RDW variations. A 5% contamination is readily detected. This approach is rapid, sensitive and reliable. Incidentally, it readily detects the presence of an abnormal hemoglobin.
© 1993 S. Karger AG, Basel
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Article / Publication Details
Received: October 09, 1992
Accepted: March 03, 1993
Published online: November 26, 2009
Issue release date: 1993
Number of Print Pages: 8
Number of Figures: 0
Number of Tables: 0
ISSN: 1015-3837 (Print)
eISSN: 1421-9964 (Online)
For additional information: https://www.karger.com/FDT
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Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher.Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
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