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Original Article

Effect of Follicular Fluid Oxidative Stress on Meiotic Spindle Formation in Infertile Women with Polycystic Ovarian Syndrome

Chattopadhayay R.a · Ganesh A.b · Samanta J.a · Jana S.K.b · Chakravarty B.N.a · Chaudhury K.b

Author affiliations

aInstitute of Reproductive Medicine, Salt Lake, Kolkata, and bSchool of Medical Science and Technology, Indian Institute of Technology, Kharagpur, India

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Gynecol Obstet Invest 2010;69:197–202

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Article / Publication Details

First-Page Preview
Abstract of Original Article

Received: May 25, 2009
Accepted: October 12, 2009
Published online: December 24, 2009
Issue release date: April 2010

Number of Print Pages: 6
Number of Figures: 0
Number of Tables: 3

ISSN: 0378-7346 (Print)
eISSN: 1423-002X (Online)

For additional information: https://www.karger.com/GOI

Abstract

Background: The effect of follicular fluid (FF) oxidative stress (OS) on meiotic spindle (MS) formation in oocytes and subsequent outcome in women with polycystic ovarian syndrome (PCOS) are evaluated in this study. Methods: 326 oocytes from 35 PCOS women (group A) and 208 oocytes from 32 women with tubal infertility (group B) were visualized for MS using PolScope. FF was analyzed for OS markers including reactive oxygen species (ROS), lipid peroxidation and total antioxidant capacity (TAC). Group A was further classified into groups A1 and A2, and group B into groups B1 and B2 depending upon the presence or absence of MS, respectively. Results: MS formation was absent in a significantly higher number of oocytes in group A compared to group B (p ≤ 0.05). OS markers were significantly higher in group A compared to group B (p ≤ 0.05). Fertilization rate, number of good quality embryos and clinical pregnancy rates were higher in group B compared to group A, though not statistically significant. FF ROS was significantly higher and TAC significantly lower in groups A2 and B2 compared to groups A1 and B1 (p < 0.001). Conclusion: The presence of MS and oocyte maturation in PCOS women showed a positive correlation with low levels of OS.

© 2009 S. Karger AG, Basel


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Article / Publication Details

First-Page Preview
Abstract of Original Article

Received: May 25, 2009
Accepted: October 12, 2009
Published online: December 24, 2009
Issue release date: April 2010

Number of Print Pages: 6
Number of Figures: 0
Number of Tables: 3

ISSN: 0378-7346 (Print)
eISSN: 1423-002X (Online)

For additional information: https://www.karger.com/GOI


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