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Original Paper

Open Access Gateway

Potential Adjuvant Effects of Nigella sativa Seeds to Improve Specific Immunotherapy in Allergic Rhinitis Patients

Işık H.a · Çevikbaş A.a · Gürer Ü.S.a · Kıran B.c · Üresin Y.d · Rayaman P.a · Rayaman E.a · Gürbüz B.a · Büyüköztürk S.b

Author affiliations

aDepartment of Pharmaceutical Microbiology, Faculty of Pharmacy, Marmara University, bDepartment of Internal Medicine, Istanbul Faculty of Medicine, Istanbul University, cInstitute of Experimental and Medical Research, Istanbul University, and dDepartment of Pharmacology and Clinical Pharmacology, Istanbul University, Istanbul, Turkey

Corresponding Author

Ümran Soyoğul Gürer

Department of Pharmaceutical Microbiology, Faculty of Pharmacy

University of Marmara, Haydarpasa, TR–34668 Istanbul (Turkey)

Tel. +90 216 414 29 62/101, Fax +90 216 345 29 52

E-Mail umran.gurer@superonline.com

Related Articles for ""

Med Princ Pract 2010;19:206–211

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Abstract

Objective: To investigate the effects of Nigella sativa seed supplementation on symptom levels, polymorphonuclear leukocyte (PMN) functions, lymphocyte subsets and hematological parameters of allergic rhinitis. Subjects and Methods: Twenty-four patients randomly selected from an experimental group of 31 (mean age 34 years) sensitive to house dust mites with allergic rhinitis and a control group of 8 healthy volunteers (mean age 23 years) were treated with allergen-specific immunotherapy in conventional doses for 30 days. After a month of immunotherapy, 12 of the 24 patients and the 8 healthy volunteers were given N. sativa seed supplementation (2 g/day orally) for 30 days. The remaining 12 patients continued only on immunotherapy during the same period. The other 7 patients were given 0.1 ml saline solution subcutaneously once a week as a placebo. The symptom scores, PMN functions, lymphocyte subsets and other hematological parameters were evaluated before and after all treatment periods. Results: There was a statistically significant increase in the phagocytic and intracellular killing activities of PMNs of patients receiving specific immunotherapy, especially after the addition of N. sativa seed. The CD8 counts of patients receiving specific immunotherapy plus N. sativa seed supplementation significantly increased compared to patients receiving only specific immunotherapy. PMN functions of healthy volunteers significantly increased after N. sativa seed supplementation compared to baseline. Conclusion:N. sativa seed supplementation during specific immunotherapy of allergic rhinitis may be considered a potential adjuvant therapy.

© 2010 S. Karger AG, Basel


References

  1. El-Dakhakhny M, Barakat M, El-Halim MA, Aly SM: Effect of N. sativa oil on gastric secretion and ethanol induced ulcer in rats. J Ethnopharmacol 2000;72:299–302.
  2. Salem ML: Immunomodulatory and immunotherapeutic properties of the Nigella sativa L. seed. Int Immunopharmacol 2005;5:1749–1770.
  3. Chakravarty N: Inhibition of histamin release from mast cells by nigellone. Ann Allergy 1993;70:237–242.
  4. Swamy SMK, Tan BKH: Cytotoxic and immunopotentiating effects of ethanolic extract of Nigella sativa L. seeds. J Ethnopharmacol 2000;70:1–7.
  5. Burits M, Bucar F: Antioxidant activity of Nigella sativa essential oil. Phytother Res 2000;14:323–328.
  6. Haq A, Abdullatif M, Lobo IP, Khabar SAK, Sheth UK, Al-Sedairy TS: Nigella sativa: effect on human lymphocytes and polymorphonuclear leucocyte phagocytic activity. Immunopharmacology 1995;30:147–155.
  7. Büyüköztürk S, Gelincik A, Ozşeker F, Genç S, Savran FO, Kiran B, Yillar G, Erden S, Aydin F, Colakoğlu B, Dal M, Ozer H, Bilir A: Nigella sativa (black seed) oil does not affect the T-helper 1 and T-helper 2 type cytokine production from splenic mononuclear cells in allergen sensitized mice. J Ethnopharmacol 2005;100:295–298.
  8. Abbas AT, Abdel-Aziz MM, Zalata KR, Abd Al-Galel Tel-D: Effect of dexamethasone and Nigella sativa on peripheral blood eosinophil count, IgG1 and IgG2a, cytokine profiles and lung inflammation in murine model of allergic asthma. Egypt J Immunol 2005;12:95–102.
    External Resources
  9. El Gazzar M, El Mezayen R, Marecki JC, Nicolls MR, Canastar A, Dreskin SC: Anti-inflammatory effect of thymoquinone in a mouse model of allergic lung inflammation. Int Immunopharmacol 2006;6:1135–1142.
  10. El Mezayen R, El Gazzar M, Nicolls MR, Marecki JC, Dreskin SC, Nomiyama H: Effect of thymoquinone on cyclooxygenase expression and prostaglandin production in a mouse model of allergic airway inflammation. Immunol Lett 2006;106:72–81.
  11. Kalus U, Pruss A, Bystron J, Jurecka M, Smekalova A, Lichius JJ, Kiesewetter H: Effect of Nigella sativa (black seed) on subjective feeling in patients with allergic diseases. Phytother Res 2003;17:1209–1214.
  12. Bousquet J, Lockey RF, Malling HJ (eds): WHO position paper. Allergen immunotherapy: therapeutic vaccines for allergic diseases. Allergy 1998;53(suppl 44):1–42.
  13. Boyum A: Isolation of molecular cells and granulocyte from human blood: isolation of mononuclear cells by one centrifugation and granulocyte by combining centrifugation and sedimentation at 1 g. Scand J Clin Lab Invest 1968;97:77–89.
  14. Alexander JW, Windhorst DB, Good RA: Improved tests for the evolution of neutrophil function in human disease. J Lab Clin Med 1968;72:136–148.
  15. Barbior BM, Cohen HJ: Measurement of neutrophil function: phagocytosis, degranulation, the respiratory burst and bacterial killing; in Cline MJ (ed): Methods in Hematology, Leukocyte Function, ed 1. New York, Churchill Livingstone, 1981, pp 1–38.
  16. Gocer P, Gurer SU, Erten N, Palanduz S, Rayaman E, Akarsu B, Karan A, Cevikbas A: Comparison of polymorphonuclear leukocyte functions in elderly patients and healthy young volunteers. Med Princ Pract 2005;14:382–385.
  17. Richardson MD, Scott G., Shankland GS: Effect of cilofungin on phagocytosis and intracellular killing of Candida albicans by human neutrophils. Eur J Clin Microbiol Infect Dis 1992;11:22–26.
  18. Gurer SU, Gocer P, Ercag E, Erten N, Rayaman E, Gurbuz B, Uzer A, Karan A, Cevikbas A: The effects of some antibiotics on polymorphonuclear leukocyte functions of elderly patients in vitro before and after zinc supplementation. Int Immunopharmacol 2006;5:808–816.
    External Resources
  19. Matricardi PM, Bonini S: Mimicking microbial ‘education’ of the immune system: a strategy to revert the epidemic trend of atopy and allergic asthma? Respir Res 2000;1:129–132.
  20. Haq A, Lobo IP, Al-Tufail M, Rama RN, Al Sedairy TS: Immunomodulatory effect of Nigella sativa proteins fractionated by ion exchange chromatography. Int J Immunopharmacol1999;21:283–295.

Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: November 27, 2008
Accepted: April 14, 2009
Published online: March 29, 2010
Issue release date: March 2010

Number of Print Pages: 6
Number of Figures: 1
Number of Tables: 3

ISSN: 1011-7571 (Print)
eISSN: 1423-0151 (Online)

For additional information: https://www.karger.com/MPP


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