Nephron Experimental Nephrology
Original Paper
Transcriptome Analysis of the Response of Cultured Murine Podocytes to Puromycin AminonucleosideKang H.G.a–c · Paik K.H.d · Cho H.Y.a · Lee B.H.a, b · Ha I.S.a, b · Choi Y.a, b · Cheong H.I.a–caDepartment of Pediatrics, Seoul National University Children’s Hospital, bKidney Research Institute, Medical Research Center, Seoul National University College of Medicine, cResearch Center for Rare Diseases, and dDepartment of Pediatrics, Samsung Medical Center, Sungkyunkwan University College of Medicine, Seoul, Korea
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Article / Publication Details
Received: May 26, 2009
Accepted: August 20, 2009
Published online: February 20, 2010
Issue release date: April 2010
Number of Print Pages: 1
Number of Figures: 3
Number of Tables: 2
eISSN: 1660-2129 (Online)
For additional information: https://www.karger.com/NEE
Abstract
Aims: Idiopathic nephrotic syndrome is known as a disease of the renal glomerular epithelial cells (podocytes). Recent advances in podocyte biology showed that podocytopathy is the culprit of nephrotic syndrome. To obtain comprehensive information about the response of podocytes to injury, we investigated the gene expression profile of podocytes in response to puromycin aminonucleoside (PAN)-induced injury. Methods: Differentiated mouse podocyte cell line (MPC5) cells were treated with 25 µg/ml PAN for 24, 48, or 72 h. Gene expression profiles of these cells were analyzed. Real time PCR analysis was used to confirm the findings of microarray. Results: Expression levels of 23 genes (differentially expressed genes, DEGs), including laminin α1 and MMP3, were significantly different between PAN-treated podocytes and untreated cells. Gene ontology of DEGs indicated that their functional categories were cell adhesion, extracellular matrix (ECM) formation, and ECM degradation. Real-time PCR and indirect immunohistochemistry of PAN-treated and untreated podocytes confirmed the differential expression of DEGs. Conclusion: Using unbiased global gene expression profiling, we found that podocytes respond to PAN-induced injury by down-regulating the expression of genes involved in cell adhesion and extracellular matrix.
© 2010 S. Karger AG, Basel
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Article / Publication Details
Received: May 26, 2009
Accepted: August 20, 2009
Published online: February 20, 2010
Issue release date: April 2010
Number of Print Pages: 1
Number of Figures: 3
Number of Tables: 2
eISSN: 1660-2129 (Online)
For additional information: https://www.karger.com/NEE
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