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Clinical Study

Effect of Human Colorectal Carcinogenesis on the Neural Cell Adhesion Molecule Expression and Polysialylation

Fernández-Briera A.a · García-Parceiro I.a · Cuevas E.b · Gil-Martín E.a

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aDepartment of Biochemistry, Genetics and Immunology, Faculty of Biology, University of Vigo, Vigo, and bPathology Service, University Hospital Complex, Ourense, Spain

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Oncology 2010;78:196–204

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Article / Publication Details

First-Page Preview
Abstract of Clinical Study

Received: July 08, 2009
Accepted: December 21, 2009
Published online: April 23, 2010
Issue release date: June 2010

Number of Print Pages: 9
Number of Figures: 2
Number of Tables: 4

ISSN: 0030-2414 (Print)
eISSN: 1423-0232 (Online)

For additional information: https://www.karger.com/OCL

Abstract

Objective: Although downregulation of neural cell adhesion molecule (NCAM) has been correlated with poor prognosis in colorectal cancer (CRC), it is also possible that colon cancer spreading comes from reducing tumor cell adhesion through NCAM polysialylation, as occurs in lung carcinoma or Wilms’ tumor. Methods: To prove this hypothesis, we have performed a prospective study on tumor and control specimens from 39 CRC patients, which were immunostained for NCAM and PSA (polysialic acid) expression. Results: Tumor versus control expression of NCAM and PSA epitopes in tissue specimens, as well as correlation between tumor expression and clinicopathological features, were statistically analyzed. Results showed a low constitutive expression of NCAM and PSA (PSA-NCAM) in control tissue, which reached a statistically significant increase in the tumor tissue. Likewise, the presence and number of lymph node metastases at surgery were correlated with NCAM expression and PSA/NCAM coexpression. Conclusions: These data highlight the importance of taking into account PSA-associated epitopes when dealing with NCAM cell expression studies in tumor development and progression. The analysis of PSA and NCAM expression in CRC suggests a new way, other than downregulation of NCAM, in order to escape contact inhibition and promote cell tumor spreading in colorectal cancer.

© 2010 S. Karger AG, Basel


References

  1. Cavallaro U, Christofori G: Cell adhesion in tumor invasion and metastasis: loss of the glue is not enough. Biochim Biophys Acta 2001;1552:39–45.
  2. Goridis C, Brunet JF: NCAM: structural diversity, function and regulation of expression. Semin Cell Biol 1992;3:189–197.
  3. Burg MA, Halfter W, Cole GJ: Analysis of proteoglycan expression in developing chicken brain: characterization of a heparin sulphate proteoglycan that interacts with the neural cell adhesion molecule. J Neurosci Res 1995;41:49–64.
  4. Walsh FS, Doherty P: Neural cell adhesion molecules of the immunoglobulin superfamily: role in axon growth and guidance. Annu Rev Cell Dev Biol 1997;13:425–456.
  5. Panicker AK, Buhusi M, Thelen K, Maness PF: Cellular signaling mechanisms of neural cell adhesion molecules. Front Biosci 2003;8:900–911.
  6. Cavallaro U, Niedermeyer J, Fuxa M, Christofori G: N-CAM modulates tumor-cell adhesion to matrix by inducing FGF-receptor signalling. Nat Cell Biol 2001;3:650–657.
  7. Conacci-Sorrell ME, Ben-Yedidia T, Shtutman M, Feinstein E, Einart P, Ben-Ze’ev A: Nr-CAM is a target gene of the β-catenin/LEF-1 pathway in melanoma and colon cancer and its expression enhances motility and confers tumorigenesis. Genes Dev 2002;16:2058–2072.
  8. Logan CY, Nusse R: The Wnt signalling pathway in development and disease. Annu Rev Cell Dev Biol 2004;20:781–810.
  9. Inoue S, Inoue Y: A challenge to the ultrasensitive chemical method for the analysis of oligo- and polysialic acids at a nanogram level of colominic acid and a milligram level of brains tissues. Biochimie 2001;83:605–613.
  10. Zuber C, Lackie PM, Catterall WA, Roth J: Polysialic acid is associated with sodium channels and the neural cell adhesion molecule N-CAM in adult rat brain. J Biol Chem 1992;267:9965–9971.
  11. Yabe U, Sato C, Matsuda T, Kitajima K: Polysialic acid in human milk. J Biol Chem 2003;278:13875–13880.
  12. Suzuki M, Angata K, Nakayama J, Fukuda M: Polysialic acid and mucin type O-glycans on the neural cell adhesion molecule differentially regulate myoblast fusion. J Biol Chem 2003;278:49459–49468.
  13. Martersteck CM, Kedersha NL, Drapp DA, Tsui TG, Colley KJ: Unique α2,8-polysialylated glycoproteins in breast cancer and leukaemia cells. Glyobiology 1996;6:289–301.
  14. Harduin-Lepers A, Vallejo-Ruiz V, Krzewinski-Recchi M, Samyn-Petit B, Julien S, Delannoy P: The human sialyltransferase family. Biochimie 2001;83:727–737.
  15. Rothbard JB, Brackenbury R, Cunningham BA, Edelman GM: Differences in the carbohydrate structures of neural cell-adhesion molecules from adult and embryonic chicken brains. J Biol Chem 1982;257:11064–11069.
  16. Franceschini I, Angata K, Ong E, Hong A, Doherty, Fukuda M: Polysialyltransferase ST8Sia II (STX) polysialylates all of the major isoforms of NCAM and facilitates neurite outgrowth. Glycobiology 2001;11:231–239.
  17. Rutishauser U: Polysialic acid at the cell surface: biophysics in service of cell interactions and tissue plasticity. J Cell Biochem 1998;70:304–312.
  18. Fujimoto I, Bruses JL, Rutishauser U: Regulation of cell adhesion by polysialic acid. Effects on cadherin, immunoglobulin cell adhesion molecule, and integrin function and independence from neural cell adhesion molecule binding or signalling activity. J Biol Chem 2001;276:31745–31751.
  19. Storms SD, Rutishauser U: A role for polysialic acid in neural cell adhesion molecule heterophilic binding to proteoglycans. J Biol Chem 1998;273:27124–27129.
  20. Roth J, Zuber C, Wagner P, Taatjes DJ, Weisgerber C, Heitz PU, Goridis C, Bitter-Suermann D: Reexpression of poly(sialic acid) units of the neural cell adhesion molecule in Wilms tumor. Proc Natl Acad Sci USA 1988;85:2999–3003.
  21. Hildebrandt H, Becker C, Gluer S, Rosner H, Gerardy-Schahn R, Rahmann H: Polysialic acid on the neural cell adhesion molecule correlates with expression of polysialyltransferases and promotes neuroblastoma cell growth. Cancer Res 1998;58:779–784.
  22. Smith SR, Auerbach B, Morgan L: Serum neural cell adhesion molecule in multiple myeloma and other plasma cell disorders. Br J Haematol 1996;92:67–70.
  23. Miyahara R, Tanaka F, Nakagawa T, Matsuoka K, Isii K, Wada H: Expression of neural cell adhesion molecules (polysialylated form of neural cell adhesion molecule and L1-cell adhesion molecule) on resected small cell lung cancer specimens: in relation to proliferation state. J Surg Oncol 2001;77:49–54.
  24. Tanaka F, Otake Y, Nakagawa T, Kawano Y, Miyahara R, Li M, Yanagihara K, Nakayama J, Fujimoto I, Ikenaka K, Wada H: Expression of polysialic acid and STX, a human polysialyltransferase, is correlated with tumor progression in non-small cell lung carcinoma. Cancer Res 2000;60:3072–3080.
  25. Tanaka F, Otake Y, Nakagawa T, Kawano Y, Miyahara R, Li M, Yanagihara K, Inui K, Oyanagi H, Yamada T, Nakayama J, Fujimoto I, Ikenaka K, Wada H: Prognostic significance of polysialic acid expression in resected non-small cell lung cancer. Cancer Res 2001;61:1666–1670.
  26. Moolenaar CE, Muller EJ, Schol DJ, Figdor CG, Bock E, Bitter-Suermann D, Michalides RJ: Expression of neural cell adhesion molecule-related sialoglycoprotein in small cell lung cancer and neuroblastoma cell lines H69 and CHP-212. Cancer Res 1990;50:1102–1106.
  27. Komminoth P, Roth J, Lackie PM, Bitter- Suermann D, Heitz PU: Polysialic acid of the neural cell adhesion molecule distinguishes small cell lung carcinoma from carcinoids. Am J Pathol 1991;139:297–304.
  28. Scheidegger EP, Lackie PM, Papay J, Roth J: In vitro and in vivo growth of clonal sublines of human small cell lung carcinoma is modulated by polysialic acid of the neural cell adhesion molecule. Lab Invest 1994;70:95–106.
  29. Lantuejoul S, Moro D, Michalides RJAM, Brambilla C, Brambilla E: Neural cell adhesion molecules (NCAM) and NCAM-PSA expression in neuroendocrine lung tumors. Am J Surg Pathol 1998;22:1267–1276.
  30. Suzuki M, Suzuki M, Nakayama J, Suzuki A, Angata K, Chen S, Sakai K, Hagihara K, Yamaguchi Y, Fukuda M: Polysialic acid facilitates tumor invasion by glioma cells. Glycobiology 2005;15:887–894.
  31. Edvardsen K, Pedersen PH, Bjerkvig R, Hermann GG, Zeuthen J, Laerum OD, Walsh FS, Bock E: Transfection of glioma cells with the neural cell adhesion NCAM: effect on glioma-cell invasion and growth in vivo. Int J Cancer 1994;58:116–122.
  32. Sasaki H, Yoshida K, Ikeda E, Asou H, Inaba M, Otani M, Kawase T: Expression of the neural cell adhesion molecule in astrocytic tumors: an inverse correlation with malignancy. Cancer 1998;82:1921–1931.
  33. Perl AK, Dahl U, Wilgenbus P, Cremer H, Semb H, Christofori G: Reduced expression of neural cell adhesion molecule induces metastasic dissemination of pancreatic beta tumor cells. Nat Med 1999;5:286–291.
  34. Roesler J, Srivatsan E, Moatamed F, Peters J, Livingston E: Tumor suppressor activity of neural cell adhesion molecule in colon carcinoma. Am J Surg 1997;174:251–257.
  35. Sampson-Johannes A, Wang W, Shtivelmen E: Colonization of human lung grafts in SCID-hu mice by human colon carcinoma cells. Int J Cancer 1996;65:864–869.
  36. Lantuéjoul S, Laverriére MH, Sturm N, Moro D, Frey G, Brambilla C, Brambilla E: NCAM (neural cell adhesion molecules) expression in malignant mesotheliomas. Hum Pathol 2000;31:415–421.
  37. Trouillas J, Daniel L, Guigard MP, Tong S, Gouvernet J, Jouanneau E, Jan M, Perrin G, Fischer G, Tabarin A, Rougon G, Figarella-Branger D: Polysialylated neural cell adhesion molecules expressed in human pituitary tumors and related to extrasellar invasion. J Neurosurg 2003;98:1084–1093.
  38. Troy F: The chemistry and biosynthesis of selected bacterial capsular polymers. Ann Rev Microbiol 1979;33:519–560.
  39. Kontogianni K, Nicholson AG, Butcher D, Sheppard MN: CD56: a useful tool for the diagnosis of small cell lung carcinomas on biopsies with extensive crush artefact. J Clin Pathol 2005;58:978–980.
  40. Onodera H, Nagayama S, Tachibana T, Fujimoto A, Imamura M: Brain metastasis from colorectal cancer. Int J Colorectal Dis 2005;20:57–61.
  41. Dall’Olio F, Chiricolo M: Sialyltransferases in cancer. Glycoconjugate J 2001;18:841–850.
  42. Seidenfaden R, Krauter A, Schertzinger F, Gerardy-Schahn R, Hildebrandt H: Polysialic acid directs tumor cell growth by controlling heterophilic neural cell adhesion molecule interactions. Mol Cell Biol 2003;23:5908–5918.
  43. Kiss JZ, Rougon G: Cell biology of polysialic acid. Curr Opin Neurobiol 1997;7:640–646.
  44. Johnson CP, Fujimoto I, Rutishauser U, Leckband DE: Direct evidence that neural cell adhesion molecule (NCAM) polysialylation increases intermembrane repulsion and abrogates adhesion. J Biol Chem 2005;280:137–145.
  45. Zhang S, Cordon-Cardo C, Zhang HS, Reuter VE, Adluri S, Hamilton WB, Lloyd KO, Livingston PO: Selection of tumor antigens as targets for immune attack using immunohistochemistry. I. Focus on gangliosides. Int J Cancer 1997;73:42–49.
  46. Roth J, Brada D, Blaha I, Ghielmini C, Bitter-Suermann D, Komminoth P, Heitz PU: Evaluation of polysialic acid in the diagnosis of Wilms’ tumor. A comparative study on urinary tract tumors and non-neuroendocrine tumors. Virchows Arch [B] 1998;56:95–102.
    External Resources
  47. Dohi T, Hashiguchi M, Yamamoto S, Zorita H, Oshima M: Fucosyltransferase-producing Sialyl Lea and Sialyl Lex carbohydrate antigens in benign and malignant gastrointestinal mucosa. Cancer 1994;73:1552–1561.
  48. Vázquez-Martín C, Cuevas E, Gil-Martín E, Fernández-Briera A: Correlation analysis between tumorous associated antigen sialyl-Tn expression and ST6GalNAc I activity in human colon adenocarcinoma. Oncology 2004;67:159–165.
  49. Huerta S, Srivatsan ES, Venkatesan N, Peters J, Moatamed F, Renner S, Livingston EH: Alternative mRNA splicing in colon cancer causes loss of expression of neural cell adhesion molecule. Surgery 2001;130:834–843.
  50. Zoltowska A, Stepinski J, Lewko B, Serkies K, Zamorska B, Roszkiewicz A, Izycka-Swieszewska E, Kruszewski WJ: Neural cell adhesion molecules in breast, colon and lung carcinomas. Arch Inmunol Ther Exp (Warsz) 2001;49:171–174.
  51. Tews DS, Nissen A: Expression of adhesion factors and degrading proteins in primary and secondary glioblastomas and their precursor tumors. Inv Met 1998;18:271–284.
  52. Tezel A-K, Dahl U, Wilgenbus P, Cremer H, Semb H, Christofori G: Reduced expression of neural cell adhesion molecule induces metastatic dissemination of pancreatic β tumor cells. Nat Med 1999;5:286–291.
  53. Cavallaro U, Christofori G: Multitasking in tumor progression: signalling functions of cell adhesion molecules. Ann NY Acad Sci 2004;1014:58–86.
  54. Christofori G: Changing neighbours, changing behaviour: cell adhesion molecule-mediated signalling during tumor progression. EMBO J 2003;22:2318–2323.
  55. Wimmenauer S, Keller H, Rückauer KD, Rahner S, Wolff-Vorbeck G, Kirste G, Von Kleist S, Farthman EH: Expression of CD44, ICAM-1 and N-CAM in colorectal cancer. Correlation with the tumor stage and the phenotypical characteristics of tumor-infiltrating lymphocytes. Anticancer Res 1997;17:2395–2400.
  56. Gavert N, Conacci-Sorrell M, Gast D, Schneider A, Altevogt P, Brabletz T, Ben-Ze’ev A: L1, a novel target of β-catenin signalling, transforms cells and is expressed at the invasive front of colon cancers. J Cell Biol 2005;168:633–642.
  57. Kleinschmidt-Demasters BK, Rojiani AM, Filley CM: Central and extrapontine myelinolysis: then...and now. J Neuropathol Exp Neurol 2006;65:1–11.
  58. Kenmotsu M, Gouchi A, Maruo Y, Murashima N, Hiramoto Y, Iwagaki H, Orita K: The expression of neural cell adhesion molecule (NCAM), neural invasion and recurrence patterns in rectal cancer: a study using anti-NCAM (neural cell adhesion molecule) antibody. Nippon Geka Gakkai Zasshi 1994;95:66–70.
  59. Durbec P, Cremer H: Revisiting the function of PSA-NCAM in the nervous system. Mol Neurobiol 2001;24:53–64.
  60. Weinhold B, Seidenfaden R, Röckle I, Mühlenhoff M, Schertzinger F, Conzelmann S, Marth JD, Gerardy-Schahn R, Hildebrandt H: Genetic ablation of polysialic acid causes severe neurodevelopmental defects rescued by deletion of the neural cell adhesion molecule. J Biol Chem 2005;280:42971–42977.

Article / Publication Details

First-Page Preview
Abstract of Clinical Study

Received: July 08, 2009
Accepted: December 21, 2009
Published online: April 23, 2010
Issue release date: June 2010

Number of Print Pages: 9
Number of Figures: 2
Number of Tables: 4

ISSN: 0030-2414 (Print)
eISSN: 1423-0232 (Online)

For additional information: https://www.karger.com/OCL


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