European Surgical Research
Original Paper
Ventilator-Associated Lung Injury Superposed to Oleic Acid Infusion or Surfactant Depletion: Histopathological Characteristics of Two Porcine Models of Acute Lung InjuryWang H.M.a, d · Bodenstein M.a · Duenges B.a · Ganatti S.b · Boehme S.a · Ning Y.a · Roehrig B.c · Markstaller K.aaDepartment of Anaesthesiology, bInstitute of Pathology, and cInstitute of Medical Biostatistics, Epidemiology and Informatics, Johannes Gutenberg University, Mainz, Germany; dNational Beijing Center for Drug Safety Evaluation and Research, Beijing Institute of Pharmacology and Toxicology, Beijing, PR China
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Article / Publication Details
Received: July 28, 2009
Accepted: July 02, 2010
Published online: October 01, 2010
Issue release date: December 2010
Number of Print Pages: 13
Number of Figures: 7
Number of Tables: 2
ISSN: 0014-312X (Print)
eISSN: 1421-9921 (Online)
For additional information: https://www.karger.com/ESR
Abstract
Background: The pathophysiological concept of acute lung injury (ALI) in combination with ventilator-associated lung injury (VALI) is still unclear. We characterized the histopathological features of intravenous injection of oleic acid (OAI) and lung lavage (LAV) combined with VALI. Methods: Pigs were randomized to the control, LAV or OAI group and ventilated by pressure-controlled ventilation. Measurements included: haemodynamics, spirometry, blood gas analysis, lung wet-to-dry weight ratio (W/D), total protein content in broncho-alveolar lavage fluid (BALF), and lung pathological description and scoring. Results: Five hours after lung injury induction, gas exchange was significantly impaired in both the OAI and the LAV groups. Compared to controls, we found an increase in W/D and histopathological total injury scores in both the LAV and OAI groups and an increase in BALF total protein content in the OAI group. In contrast to the LAV group, the OAI group showed septal necrosis and alveolar oedema. Both groups exhibited dorsal and caudal atelectasis and interstitial oedema. In addition, the OAI group demonstrated a propensity to dorsal necrosis and congestion whereas the LAV group tended to develop ventral overdistension and barotrauma. Conclusions: This study presents a comparison of porcine OAI and LAV models combined with VALI, providing information for study design in research on ALI.
© 2010 S. Karger AG, Basel
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Article / Publication Details
Received: July 28, 2009
Accepted: July 02, 2010
Published online: October 01, 2010
Issue release date: December 2010
Number of Print Pages: 13
Number of Figures: 7
Number of Tables: 2
ISSN: 0014-312X (Print)
eISSN: 1421-9921 (Online)
For additional information: https://www.karger.com/ESR
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