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Original Paper

Telocytes in Human Term Placenta: Morphology and Phenotype

Suciu L.a, b · Popescu L.M.a, b · Gherghiceanu M.b · Regalia T.a, b · Nicolescu M.I.a, b · Hinescu M.E.a, b · Faussone-Pellegrini M.-S.c

Author affiliations

aDepartment of Cellular and Molecular Medicine, ‘Carol Davila’ University of Medicine and Pharmacy, and b‘Victor Babeş’ National Institute of Pathology, Bucharest, Romania; cDepartment of Anatomy, Histology and Forensic Medicine, University of Florence, Florence, Italy

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Cells Tissues Organs 2010;192:325–339

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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Accepted: April 08, 2010
Published online: July 27, 2010
Issue release date: October 2010

Number of Print Pages: 15
Number of Figures: 0
Number of Tables: 0

ISSN: 1422-6405 (Print)
eISSN: 1422-6421 (Online)

For additional information: https://www.karger.com/CTO

Abstract

In the last few years, a new cell type – interstitial Cajal-like cell (ICLC) – has been described in digestive and extra-digestive organs. The name has recently been changed to telocytes (TC) and their typical thin, long processes have been named telopodes (TP). To support the hypothesis that TC may also be present in human placenta and add to the information already available, we provide evidence on the ultrastructure, immunophenotype, distribution, and interactions with the surrounding stromal cells of TC in the villous core of human term placenta. We used phase-contrast microscopy, light microscopy of semithin sections, transmission electron microscopy, immunohistochemistry, and immunofluorescence of tissue sections or cell cultures, following a pre-established diagnostic algorithm. Transmission electron microscopy showed cells resembling TC, most (∼76%) having 2–3 very thin, longprocesses (tens to hundreds of micrometers), with an uneven calibre(≤0.5 µm thick) and typical branching pattern. The dilations of processes accommodate caveolae, endoplasmic reticulum cisternae, and mitochondria. These TC have close contacts with perivascular SMC in stem villi. In situ, similar cells are positive for c-kit, CD34, vimentin, caveolin-1, vascular endothelial growth factor (VEGF), and inducible nitric oxide synathase (iNOS). The c-kit-positive cells inconsistently co-express CD34, CD44, αSMA, S100, neuron-specific enolase, and nestin. Among cells with a morphologic TC profile in cell cultures, about 13% co-express c-kit, vimentin, and caveolin-1; 70% of the c-kit-positive cells co-express CD34 and 12% co-express iNOS or VEGF. In conclusion, this study confirms the presence of TC in human term placenta and provides their ultrastructural and immunophenotypic characterization.

© 2010 S. Karger AG, Basel


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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Accepted: April 08, 2010
Published online: July 27, 2010
Issue release date: October 2010

Number of Print Pages: 15
Number of Figures: 0
Number of Tables: 0

ISSN: 1422-6405 (Print)
eISSN: 1422-6421 (Online)

For additional information: https://www.karger.com/CTO


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