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Research Article

Editor's Choice - Free Access

An Antisense Transcript from within the ptsG Promoter Region in Escherichia coli

Pennetier C.a · Oberto J.b · Plumbridge J.a

Author affiliations

aUPR9073-CNRS Institut de Biologie Physico-Chimique, Paris, bUMR8621-CNRS Institut de Génétique et Microbiologie, Université Paris X1, Orsay, France

Corresponding Author

Jacqueline Plumbridge

UPR9073-CNRS Institut de Biologie Physico-Chimique

13 rue Pierre et Marie Curie

FR–75005 Paris (France)

Tel. +33 1 58 41 51 52, Fax +33 1 58 41 50 20, E-Mail Jackie.Plumbridge@ibpc.fr

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J Mol Microbiol Biotechnol 2010;18:230–240

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Abstract

The ptsG gene, encoding the major glucose uptake system in Escherichia coli, is expressed from 2 promoters, a minor promoter p2 and a major downstream promoter p1. Transcription from both promoters is repressed by Mlc, and expression of p1 is activated by the cAMP/catabolite activator protein complex. Expression from p1 is also regulated post-transcriptionally in response to sugar stress via an sRNA, SgrS, which results in translational inhibition and mRNA degradation. Here, we demonstrate an additional level of complexity to the transcriptional pattern surrounding ptsG. A third promoter, p3, located between p1 and p2, was found to express a transcript antisense to ptsG. This promoter was detected by in vitro transcription and by RNA polymerase footprinting techniques and in vivo by S1 analysis and fusions with a lacZ reporter gene. Although the intrinsic strength of the p3 promoter was comparable to that of ptsG, it proved difficult to identify a full-length transcript. A faint transcript of greater than 400 nt could be detected. The transcript thus has more of the characteristics of a divergently expressed cryptic unstable transcript (CUT) than a prokaryotic sRNA.

© 2010 S. Karger AG, Basel


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Article / Publication Details

First-Page Preview
Abstract of Research Article

Published online: July 29, 2010
Issue release date: August 2010

Number of Print Pages: 11
Number of Figures: 5
Number of Tables: 1

ISSN: 1464-1801 (Print)
eISSN: 1660-2412 (Online)

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