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Coagulation in Invertebrates

Cerenius L. · Söderhäll K.

Author affiliations

Department of Comparative Physiology, Uppsala University, Uppsala, Sweden

Corresponding Author

Dr. Kenneth Söderhäll

Department of Comparative Physiology, Uppsala University

Norbyvägen 18A

SE–75236 Uppsala (Sweden)

Tel. +46 18 417 2818, Fax +46 18 471 6425, E-Mail Kenneth.Soderhall@ebc.uu.se

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J Innate Immun 2011;3:3–8

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In most animals there is a need to quickly prevent the loss of blood or equivalent fluids through inflicted injuries. In invertebrates with an open circulatory system (and sometimes a hydroskeleton as well) these losses may otherwise soon be fatal. Also, there is a need to prevent microbes that have gained access to the body through the wound from disseminating throughout the open circulatory system. Therefore, many invertebrates possess a coagulation system to prevent such accidents from having too serious consequences. In this review we discuss recent developments in a few animals – mainly arthropods – where more detailed data are available. It is likely, however, that corresponding systems are present in most phyla, but this is still unchartered territory.

© 2010 S. Karger AG, Basel


  1. Hillier BJ, Vacquier VD: Amassin, an olfactomedin protein, mediates the massive intercellular adhesion of sea urchin coelomocytes. J Cell Biol 2003;160:597–604.
  2. Cavaliere V, Papademetrio DL, Alvarez EM, Blanco GA: Haemostatic and immune role of cellular clotting in the sipunculan Themiste petricola. Cell Tissue Res 2010;339:597–611.
  3. Kawabata S, Muta T: Sadaaki Iwanaga: discovery of the lipopolysaccharide- and β-1,3-D-glucan-mediated proteolytic cascade and unique proteins in invertebrate immunity. J Biochem 2010;147:611–618.
  4. Hall M, Wang R, Antwerpen RV, Sottrup-Jensen L, Söderhäll K: The crayfish plasma clotting protein: a vitellogenin-related protein responsible for clot formation in crustacean blood. Proc Natl Acad Sci USA 1999;96:1965–1970.
  5. Kawabata S, Koshiba T, Shibata T: The lipopolysaccharide-activated innate immune response network of the horseshoe crab. Invertebr Surv J 2009;6:59–77.
  6. Dushay MS: Insect hemolymph clotting. Cell Mol Life Sci 2009;66:2643–2650.
  7. Cerenius L, Kawabata S, Lee BL, Nonaka M, Söderhäll K: Proteolytic cascades and their involvement in invertebrate immunity. Trends Biochem Sci 2010;35:578–583.
    External Resources
  8. Theopold U, Schmidt O, Söderhäll K, Dushay MS: Coagulation in arthropods: defense, wound closure and healing. Trends Immunol 2004;25:289–294.
  9. Jiang Y, Doolittle RF: The evolution of vertebrate blood coagulation as viewed from a comparison of puffer fish and sea squirt genomes. Proc Natl Acad Sci USA 2003;100:7527–7532.
  10. Ariki S, Koori K, Osaki T, Motoyama K, Inamori K, Kawabata S: A serine protease zymogen functions as a pattern-recognition receptor for lipopolysaccharides. Proc Natl Acad Sci USA 2004;101:953–958.
  11. Takaki Y, Seki N, Kawabata S, Iwanaga S, Muta T: Duplicated binding sites for 1,3-β-D-glucan in the horseshoe crab coagulation factor G: implications for a molecular basis of the pattern recognition in innate immunity. J Biol Chem 2002;277:14281–14287.
  12. Matsuda Y, Osaki T, Hashii T, Koshiba T, Kawabata S: A cysteine-rich protein from an arthropod stabilizes clotting mesh and immobilizes bacteria at injury sites. J Biol Chem 2007;282:33545–33552.
  13. Yeh MS, Huang CJ, Leu JH, Lee YC, Tsai IH: Molecular cloning and characterization of a hemolymph clottable protein from tiger shrimp (Penaeus monodon). Eur J Biochem 1999;266:624–633.
  14. Perazzolo LM, Lorenzini DM, Daffre S, Barracco M: Purification and partial characterization of the plasma clotting protein from the pink shrimp Farfantepenaeus paulensis. Comp Biochem Physiol B Biochem Mol Biol 2005;142:302–307.
  15. Yeh MS, Huang CJ, Cheng JH, Tsai IH: Tissue specific expression and regulation of the hemolymph clottable protein of tiger shrimp (Penaeus monodon). Fish Shellfish Immunol 2007;23:272–279.
  16. Maningas MB, Kondo H, Hirono I, Saito-Taki T, Aoki T: Essential function of transglutaminase and clotting protein in shrimp immunity. Mol Immunol 2008;45:1269–1275.
  17. Wang R, Liang Z, Hall M, Söderhäll K: A transglutaminase involved in the coagulation system of the freshwater crayfish Pacifastacus leniusculus: tissue localization and cDNA cloning. Fish Shellfish Immunol 2001;11:623–637.
  18. Yeh MS, Kao LR, Huang CJ, Tsai IH: Biochemical characterization and cloning of transglutaminases responsible for hemolymph clotting in Penaeus monodon. Biochim Biophys Acta 2006;1764:1167–1178.
  19. Lin X, Söderhäll K, Söderhäll I: Transglutaminase activity in the hematopoietic tissue of a crustacean, Pacifastacus leniusculus: importance in hemocyte homeostasis. BMC Immunol 2008;9:58.
  20. Söderhäll I, Kim YA, Jiravanichpaisal P, Lee SY, Söderhäll K: An ancient role for a prokineticin domain in invertebrate hematopoiesis. J Immunol 2005;174:6153–6160.
    External Resources
  21. Watthanasurorot A, Söderhäll K, Jiravanichpaisal P, Söderhäll I: An ancient cytokine, astakine, mediates circadian regulation of invertebrate hematopoiesis. Cell Mol Life Sci 2010, Epub ahead of print.
  22. Lindgren M, Riazi R, Lesch C, Wilhelmsson C, Theopold U, Dushay MS: Fondue and transglutaminase in the Drosophila larval clot. J Insect Physiol 2008;54:586–592.
  23. Scherfer C, Qazi MR, Takahashi K, Ueda R, Dushay MS, Theopold U, Lemaitre B: The Toll immune-regulated Drosophila protein Fondue is involved in hemolymph clotting and puparium formation. Dev Biol 2006;295:156–163.
  24. Bidla G, Lindgren M, Theopold U, Dushay MS: Hemolymph coagulation and phenoloxidase in Drosophila larvae. Dev Comp Immunol 2005;29:669–679.
  25. Lesch C, Goto A, Lindgren M, Bidla G, Dushay MS, Theopold U: A role for Hemolectin in coagulation and immunity in Drosophila melanogaster. Dev Comp Immunol 2007;31:1255–1263.
  26. Matsuda Y, Koshiba T, Osaki T, et al: An arthropod cuticular chitin-binding protein endows injured sites with transglutaminase-dependent mesh. J Biol Chem 2007;282:37316–37324.
  27. Wang Z, Wilhelmsson C, Hyrsl P, Loof TG, Dobes P, Klupp M, Loseva O, Mörgelin M, Iklé J, Cripps RM, Herwald H, Theopold U: Pathogen entrapment by transglutaminase – a conserved innate immune mechanism. PLoS Pathog 2010;6:e1000763.
  28. Hyrsl P, Dobes P, Wang Z, Hauling T, Wilhelmsson C, Theopold U: Clotting factors and eicosanoids protect against nematode infections. J Innate Immun 2010;3:65–70.
    External Resources
  29. Babcock DT, Brock AR, Fish GS, Wang Y, Perrin L, Krasnow MA, Galko MJ: Circulating blood cells function as a surveillance system for damaged tissue in Drosophila larvae. Proc Natl Acad Sci USA 2008;105:10017–10022.
  30. Nakatogawa S, Oda Y, Kamiya M, Kamijima T, Aizawa T, Clark KD, Demura M, Kawano K, Strand MR, Hayakawa Y: A novel peptide mediates aggregation and migration of hemocytes from an insect. Cur Biol 2009;19:779–785.
  31. Altincicek B, Stötzel S, Wygrecka M, Preissner KT, Vilcinskas A: Host-derived extracellular nucleic acids enhance innate immune responses, induce coagulation, and prolong survival upon infection in insects. J Immunol 2008;2705–2712.

Article / Publication Details

First-Page Preview
Abstract of Review

Received: September 17, 2010
Accepted: October 18, 2010
Published online: November 06, 2010
Issue release date: December 2010

Number of Print Pages: 6
Number of Figures: 3
Number of Tables: 0

ISSN: 1662-811X (Print)
eISSN: 1662-8128 (Online)

For additional information: https://www.karger.com/JIN

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