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Original Article

Effects of 4-Hydroxy-2-Nonenal, a Major Lipid Peroxidation-Derived Aldehyde, and N-Acetylcysteine on the Cyclooxygenase-2 Expression in Human Uterine Myometrium

Temma-Asano K.a · Tskitishvili E.a · Kanagawa T.a · Tomimatsu T.a · Tsutsui T.a · Kimura T.a · Chang Y.S.b · Nakamura T.b · Nakai Y.b · Shimoya K.b

Author affiliations

aDepartment of Obstetrics and Gynecology, Osaka University Graduate School of Medicine, Osaka, and bDepartment of Obstetrics and Gynecology, Kawasaki Medical School, Kurashiki City, Japan

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Gynecol Obstet Invest 2011;72:37–42

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Article / Publication Details

First-Page Preview
Abstract of Original Article

Received: April 19, 2010
Accepted: October 29, 2010
Published online: January 06, 2011
Issue release date: August 2011

Number of Print Pages: 6
Number of Figures: 5
Number of Tables: 0

ISSN: 0378-7346 (Print)
eISSN: 1423-002X (Online)

For additional information: https://www.karger.com/GOI

Abstract

Background: Chorioamnionitis is one of the important causes of preterm labor. Preterm labor with chorioamnionitis is associated with oxidative stress. We reported that 4-hydroxy-2-nonenal (4-HNE), a major end product of oxidative fatty acid metabolism, is accumulated in the placenta with chorioamnionitis. The aim of this study was to confirm the effect of 4-HNE on cyclooxygenase-2 (COX-2) and prostaglandin (PG) induction in the uterine myometrial tissues. We also examined the effect of N-acetylcysteine (NAC) on 4-HNE-induced COX-2 expression. Methods: Uterine myometrial tissues were obtained from 5 patients. One of them underwent elective cesarean section without labor, and 4 of them underwent hysterectomy because of placental previa or atonic bleeding. We stimulated the uterine myometrial tissues with 4-HNE. In addition, the tissues were pretreated with NAC before 4-HNE treatment. The expression of COX-2 mRNA was observed by real-time PCR. PGE2 and prostacyclin release into the supernatants of the tissue cultures was measured by ELISA. Results: 4-HNE induced the COX-2 mRNA expression and PGE2 production in the uterine myometrial tissue culture in a dose-dependent and time-dependent manner. NAC inhibited 4-HNE-induced COX-2 expression. Conclusion: 4-HNE may play an important role in preterm labor. NAC might be protective against preterm labor under oxidative stress.

© 2011 S. Karger AG, Basel


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Article / Publication Details

First-Page Preview
Abstract of Original Article

Received: April 19, 2010
Accepted: October 29, 2010
Published online: January 06, 2011
Issue release date: August 2011

Number of Print Pages: 6
Number of Figures: 5
Number of Tables: 0

ISSN: 0378-7346 (Print)
eISSN: 1423-002X (Online)

For additional information: https://www.karger.com/GOI


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