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Experimental Chemotherapy

Evaluation of the Effect of Acetyl L-Carnitine on Experimental Cisplatin Ototoxicity and Neurotoxicity

Gunes D.a · Kirkim G.c · Kolatan E.d · Guneri E.A.c · Ozogul C.f · Altun Z.b · Serbetcioglu B.c · Yilmaz O.d · Aktas S.b · Mutafoglu K.a · Tufekci O.e · Erbayraktar Z.b · Olgun N.a

Author affiliations

Departments of aPediatric Oncology and bBasic Oncology, Institute of Oncology, and Departments of cOtorhinolaryngology, dLaboratory Animal Science and ePediatrics, Faculty of Medicine, Dokuz Eylul University, Izmir, and fDepartment of Histology and Embryology, Faculty of Medicine, Gazi University, Ankara, Turkey

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Chemotherapy 2011;57:186–194

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Article / Publication Details

First-Page Preview
Abstract of Experimental Chemotherapy

Received: April 12, 2010
Accepted: November 08, 2010
Published online: April 27, 2011
Issue release date: June 2011

Number of Print Pages: 9
Number of Figures: 6
Number of Tables: 0

ISSN: 0009-3157 (Print)
eISSN: 1421-9794 (Online)

For additional information: https://www.karger.com/CHE

Abstract

Introduction: Cisplatin (CDDP) is an effective and widely used chemotherapeutic agent for pediatric tumors, and ototoxicity is one of the dose-limiting side effects. Objective: It was the aim of our study to investigate the effect of acetyl L-carnitine (ALCAR) on experimental CDDP ototoxicity by audiologic tests, histomorphologic, immunohistochemical and ultrastructural examinations and to investigate the apoptotic pathways. Materials and Methods: Wistar albino rats (n = 28) were studied. Baseline audiological tests were performed in 4 groups: group 1, control; group 2, ALCAR; group 3, CDDP; group 4, CDDP + ALCAR-administered rats. Control audiological tests were performed on the 3rd day, and then the rats were sacrificed. Ear and brain specimens were examined by transmission electron microscopy, and caspase 3, 8 and 9 activities were investigated. Results: The CDDP-administered rats showed significant auditory brainstem response threshold shifts using all stimuli (clicks, 6-kHz and 8-kHz tone burst) compared with the control groups. The CDDP + ALCAR-administered rats showed significant auditory brainstem response threshold shifts by only click stimuli compared with the control groups. In the brain, spiral ganglion and organ of Corti, ultrastructural damage was prominent in group 3; the number of TUNEL (terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling)-positive cells and caspase 3, 8 and 9 immunostaining cells was significantly high in group 3. Conclusion: ALCAR improves CDDP-induced auditory impairment, and also antioxidative and antiapoptotic properties of ALCAR on CDDP ototoxicity were supported by the findings.

© 2011 S. Karger AG, Basel


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Article / Publication Details

First-Page Preview
Abstract of Experimental Chemotherapy

Received: April 12, 2010
Accepted: November 08, 2010
Published online: April 27, 2011
Issue release date: June 2011

Number of Print Pages: 9
Number of Figures: 6
Number of Tables: 0

ISSN: 0009-3157 (Print)
eISSN: 1421-9794 (Online)

For additional information: https://www.karger.com/CHE


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