Pharmacology
Original Paper
Effects of Different Types of Anesthetic Agents on Cellular Protein Kinase C-γ Dynamics in Mouse BrainTakeda A.a · Miyashita R.a · Nagura T.a · Sekine S.a · Murozono M.a · Matsumoto S.b · Uchino H.aaDepartment of Anesthesiology, Tokyo Medical University Hospital, Tokyo, and bDepartment of Anesthesiology, Ota General Hospital, Ota City, Japan
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Article / Publication Details
Received: December 21, 2010
Accepted: January 12, 2011
Published online: March 10, 2011
Issue release date: April 2011
Number of Print Pages: 7
Number of Figures: 4
Number of Tables: 0
ISSN: 0031-7012 (Print)
eISSN: 1423-0313 (Online)
For additional information: https://www.karger.com/PHA
Abstract
Background/Aims: Although protein kinase C-γ (PKC-γ) is a target for the effects of volatile anesthetics, the molecular mechanisms of the kinase function during their action remain unclear. We examined the effects of different types of anesthetics on PKC-γ knockout mice. Furthermore, we investigated the dynamics of the kinase in brain cells obtained from mice anesthetized with these anesthetics. Methods: We measured the required times for loss of righting reflex (rtfLORR) after administration of isoflurane, sevoflurane, or propofol on PKC-γ knockout mice and compared the times with those of wild-type mice. We also used immunoblotting to investigate the intracellular distribution of PKC-γ and phosphorylated PKC-γ (p-PKC-γ) in brain cell fractions obtained from wild-type mice during the loss of righting reflex induced by these anesthetics. Results: Isoflurane (2.6%) and sevoflurane (3.4%) used at twice the minimum alveolar concentration significantly prolonged the rtfLORRs in PKC-γ knockout mice compared to those in wild-type mice. On the other hand, no significant difference was observed between knockout and wild-type mice treated with propofol (200 mg/kg). Examination of the cellular fractions isolated from volatile anesthetic-treated mouse brains showed that PKC-γ was significantly decreased in the synaptic membrane fraction (P2), whereas p-PKC-γ was significantly increased in P2. There was no significant change in the supernatant fraction (S). In propofol-treated mice, PKC-γ and p-PKC-γ showed no significant changes in P2 or S. Conclusion: Our results provide new evidence to support the possibility of the involvement of PKC-γ in the actions of volatile anesthetics.
© 2011 S. Karger AG, Basel
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Article / Publication Details
Received: December 21, 2010
Accepted: January 12, 2011
Published online: March 10, 2011
Issue release date: April 2011
Number of Print Pages: 7
Number of Figures: 4
Number of Tables: 0
ISSN: 0031-7012 (Print)
eISSN: 1423-0313 (Online)
For additional information: https://www.karger.com/PHA
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