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Original Article

Fibroblast Growth Factor-9 in Marsupial Testicular Development

Chung J.W.a, b · Pask A.J.a–c · Yu H.a, b · Renfree M.B.a, b

Author affiliations

aAustralian Research Council Centre of Excellence in Kangaroo Genomics, and bDepartment of Zoology, University of Melbourne,Vic., Australia; cDepartment of Molecular and Cellular Biology, University of Connecticut, Storrs, Conn., USA

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Sex Dev 2011;5:131–140

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Article / Publication Details

First-Page Preview
Abstract of Original Article

Accepted: February 28, 2011
Published online: April 29, 2011
Issue release date: June 2011

Number of Print Pages: 10
Number of Figures: 6
Number of Tables: 1

ISSN: 1661-5425 (Print)
eISSN: 1661-5433 (Online)

For additional information: https://www.karger.com/SXD

Abstract

FGF9 is a member of the fibroblast growth factor (FGF) family and is critical for early testicular development and germ cell survival in the mouse. Fgf9 reinforces the testis determinant Sox9 and antagonizes Wnt4, an ovarian factor. To determine whether FGF9 has a conserved role in the mammalian gonad, we examined its expression in the gonads of a marsupial, the tammar wallaby Macropus eugenii, and compared it to WNT4 expression. Marsupial FGF9 is highly conserved with orthologues from eutherian mammals, including humans. FGF9 protein was detected in both the testis and ovary before sexual differentiation, but it subsequently became sexually dimorphic during the period of testicular differentiation. The protein was specifically enriched in the seminiferous cords of the developing testis in the Sertoli and germ cells. FGF9 mRNA expression was upregulated in the tammar testis at the time of seminiferous cord formation and downregulated in the developing ovary in an opposite profile to that of marsupial WNT4. These observations suggest that FGF9 promotes male fate in the early gonad of marsupials through an antagonistic relationship with WNT4 as it does in eutherian mammals.

© 2011 S. Karger AG, Basel


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Article / Publication Details

First-Page Preview
Abstract of Original Article

Accepted: February 28, 2011
Published online: April 29, 2011
Issue release date: June 2011

Number of Print Pages: 10
Number of Figures: 6
Number of Tables: 1

ISSN: 1661-5425 (Print)
eISSN: 1661-5433 (Online)

For additional information: https://www.karger.com/SXD


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