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Original Paper

Utility of the Peripheral Blood Basophil Histamine Release Test in the Diagnosis of Hen’s Egg, Cow’s Milk, and Wheat Allergy in Children

Sato S.a · Tachimoto H.a · Shukuya A.b · Ogata M.b · Komata T.b · Imai T.b · Tomikawa M.b · Ebisawa M.a

Author affiliations

aClinical Research Center for Allergy and Rheumatology, and bDepartment of Pediatrics, Sagamihara National Hospital, Sagamihara, Japan

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Int Arch Allergy Immunol 2011;155(suppl 1):96–103

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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Published online: June 01, 2011
Issue release date: June 2011

Number of Print Pages: 8
Number of Figures: 3
Number of Tables: 2

ISSN: 1018-2438 (Print)
eISSN: 1423-0097 (Online)

For additional information: https://www.karger.com/IAA

Abstract

Background: The diagnosis of food allergy (FA) is made by oral food challenge tests (OFCs) that occasionally produce serious symptoms in patients; therefore, whether to perform OFCs should be carefully considered. The utility of the histamine release test (HRT) in the diagnosis of childhood FA has not been fully examined. Methods: Sixty-four subjects with suspected hen’s egg allergy, cow’s milk allergy (CMA), and wheat allergy (WA) were enrolled. The diagnosis of FA was made based on the outcomes of OFCs or a convincing history of symptoms after food ingestion within 6 months before or after sample collection. HRT was performed using an HRT Shionogi kit. The threshold of histamine release (HRT threshold), which was defined as the minimum concentration of food antigen to induce a 10% net histamine release, was analyzed in association with FA diagnosis. Results: Receiver operating characteristic analysis showed that the HRT threshold was useful in the diagnosis of heated egg allergy (HEA), raw egg allergy (REA), CMA, and WA. We were able to determine the cutoff value for the HRT threshold in relation to outcomes of OFCs. The cutoff value was 6 ng/ml of egg white antigen in HEA and REA (p < 0.01), 40 ng/ml of milk antigen in CMA (p < 0.01), and 500 ng/ml of wheat antigen in WA (p < 0.05). The efficiency was 70.3% for HEA, 78.0% for REA, 77.6% for CMA, and 70.7% for WA. Conclusions: We conclude that the HRT threshold measurement for egg white, milk, and wheat antigen is related to outcomes of OFCs and is useful in determining when OFCs should be performed.

© 2011 S. Karger AG, Basel


References

  1. Komata T, Soderstrom L, Borres MP, Tachimoto H, Ebisawa M: The predictive relationship of food-specific serum IgE concentrations to challenge outcomes for egg and milk varies by patient age. J Allergy Clin Immunol 2007;119:1272–1274.
  2. Sampson HA: Utility of food-specific IgE concentrations in predicting symptomatic food allergy. J Allergy Clin Immunol 2001;107:891–896.
  3. Sampson HA, Ho DG: Relationship between food-specific IgE concentrations and the risk of positive food challenges in children and adolescents. J Allergy Clin Immunol 1997;100:444–451.
  4. Skripak JM, Matsui EC, Mudd K, Wood RA: The natural history of IgE-mediated cow’s milk allergy. J Allergy Clin Immunol 2007;120:1172–1177.
  5. MacGlashan DW Jr, Peters SP, Warner J, Lichtenstein LM: Characteristics of human basophil sulfidopeptide leukotriene release: releasability defined as the ability of the basophil to respond to dimeric cross-links. J Immunol 1986;136:2231–2239.
  6. Schroeder JT, MacGlashan DW Jr, Kagey-Sobotka A, White JM, Lichtenstein LM: IgE-dependent IL-4 secretion by human basophils: the relationship between cytokine production and histamine release in mixed leukocyte cultures. J Immunol 1994;153:1808–1817.
  7. Malveaux FJ, Conroy MC, Adkinson NF Jr, Lichtenstein LM: IgE receptors on human basophils: relationship to serum IgE concentration. J Clin Invest 1978;62:176–181.
  8. MacDonald SM, Schleimer RP, Kagey-Sobotka A, Gillis S, Lichtenstein LM: Recombinant IL-3 induces histamine release from human basophils. J Immunol 1989;142:3527–3532.
  9. Boumiza R, Monneret G, Forissier MF, Savoye J, Gutowski MC, Powell WS, Bienvenu J: Marked improvement of the basophil activation test by detecting CD203c instead of CD63. Clin Exp Allergy 2003;33:259–265.
  10. de Weck AL, Sanz ML, Gamboa PM, Aberer W, Bienvenu J, Blanca M, Demoly P, Ebo DG, Mayorga L, Monneret G, Sainte-Laudy J: Diagnostic tests based on human basophils: more potentials and perspectives than pitfalls. Int Arch Allergy Immunol 2008;146:177–189.
  11. Ebo DG, Hagendorens MM, Bridts CH, Schuerwegh AJ, De Clerck LS, Stevens WJ: Flow cytometric analysis of in vitro activated basophils, specific IgE and skin tests in the diagnosis of pollen-associated food allergy. Cytometry B Clin Cytom 2005;64:28–33.
  12. Erdmann SM, Heussen N, Moll-Slodowy S, Merk HF, Sachs B: CD63 expression on basophils as a tool for the diagnosis of pollen-associated food allergy: sensitivity and specificity. Clin Exp Allergy 2003;33:607–614.
  13. Erdmann SM, Sachs B, Schmidt A, Merk HF, Scheiner O, Moll-Slodowy S, Sauer I, Kwiecien R, Maderegger B, Hoffmann-Sommergruber K: In vitro analysis of birch-pollen-associated food allergy by use of recombinant allergens in the basophil activation test. Int Arch Allergy Immunol 2005;136:230–238.
  14. Gober LM, Eckman JA, Sterba PM, Vasagar K, Schroeder JT, Golden DB, Saini SS: Expression of activation markers on basophils in a controlled model of anaphylaxis. J Allergy Clin Immunol 2007;119:1181–1188.
  15. Ocmant A, Mulier S, Hanssens L, Goldman M, Casimir G, Mascart F, Schandene L: Basophil activation tests for the diagnosis of food allergy in children. Clin Exp Allergy 2009;39:1234–1245.
  16. Platz IJ, Binder M, Marxer A, Lischka G, Valent P, Buhring HJ: Hymenoptera-venom-induced upregulation of the basophil activation marker ecto-nucleotide pyrophosphatase/phosphodiesterase 3 in sensitized individuals. Int Arch Allergy Immunol 2001;126:335–342.
  17. Wanich N, Nowak-Wegrzyn A, Sampson HA, Shreffler WG: Allergen-specific basophil suppression associated with clinical tolerance in patients with milk allergy. J Allergy Clin Immunol 2009;123:789–794.e720.
  18. Lichtenstein LM, Norman PS, Connell JT: Comparison between skin-sensitizing antibody titers and leukocyte sensitivity measurements as an index of the severity of ragweed hay fever. J Allergy 1967;40:160–167.
  19. Hamilton RG, Franklin Adkinson N Jr: In vitro assays for the diagnosis of IgE-mediated disorders. J Allergy Clin Immunol 2004;114:213–225; quiz 226.
  20. Nishi H, Nishimura S, Higashiura M, Ikeya N, Ohta H, Tsuji T, Nishimura M, Ohnishi S, Higashi H: A new method for histamine release from purified peripheral blood basophils using monoclonal antibody-coated magnetic beads. J Immunol Methods 2000;240:39–46.
  21. Norgaard A, Bindslev-Jensen C: Egg and milk allergy in adults: diagnosis and characterization. Allergy 1992;47:503–509.
  22. Bernstein M, Day JH, Welsh A: Double-blind food challenge in the diagnosis of food sensitivity in the adult. J Allergy Clin Immunol 1982;70:205–210.
  23. Clinton PM, Kemeny DM, Amlot P, Urbanek R, Lessof MH: Histamine release from peripheral blood leucocytes in egg-allergic patients. Clin Allergy 1986;16:345–354.
  24. Kleine-Tebbe J, Galleani M, Jeep S, Pilz B, Baisch A, Kunkel G: Basophil histamine release in patients with birch pollen hypersensitivity with and without allergic symptoms to fruits. Allergy 1992;47:618–623.
  25. Lau S, Thiemeier M, Urbanek R, Kemeny M, Wahn U: Immediate hypersensitivity to ovalbumin in children with hen’s egg white allergy. Eur J Pediatr 1988;147:606–608.
  26. Norgaard A, Skov PS, Bindslev-Jensen C: Egg and milk allergy in adults: comparison between fresh foods and commercial allergen extracts in skin prick test and histamine release from basophils. Clin Exp Allergy 1992;22:940–947.
  27. Hansen TK, Bindslev-Jensen C: Codfish allergy in adults: identification and diagnosis. Allergy 1992;47:610–617.
  28. Kleine-Tebbe J, Werfel S, Roedsgaard D, Nolte H, Skov PS, Wahn U, Kunkel G: Comparison of fiberglass-based histamine assay with a conventional automated fluorometric histamine assay, case history, skin prick test, and specific serum IgE in patients with milk and egg allergic reactions. Allergy 1993;48:49–53.
  29. Ebisawa M: Food Allergy Management Guidelines 2008 by the National Food Allergy Research Group Supported by the Ministry of Health, Welfare, and Labor, 2008.
  30. Ebisawa M, Akasawa A, Kunou M, Iikura Y: Establishment of diagnosis of food allergy: food challenge test by using dry powdered foods. Iryo 2000;54:79–84.
  31. Takemoto H, Nishimura S, Kosada Y, Hata S, Takagi S, Hosoi S, Ezumi K, Ide M, Harada S: Anti-human IgE monoclonal antibodies recognizing epitopes related to the binding sites of high and low affinity IgE receptors. Microbiol Immunol 1994;38:63–71.
  32. Sampson HA, Broadbent KR, Bernhisel-Broadbent J: Spontaneous release of histamine from basophils and histamine-releasing factor in patients with atopic dermatitis and food hypersensitivity. N Engl J Med 1989;321:228–232.
  33. Ando H, Moverare R, Kondo Y, Tsuge I, Tanaka A, Borres MP, Urisu A: Utility of ovomucoid-specific IgE concentrations in predicting symptomatic egg allergy. J Allergy Clin Immunol 2008;122:583–588.

Article / Publication Details

First-Page Preview
Abstract of Original Paper

Published online: June 01, 2011
Issue release date: June 2011

Number of Print Pages: 8
Number of Figures: 3
Number of Tables: 2

ISSN: 1018-2438 (Print)
eISSN: 1423-0097 (Online)

For additional information: https://www.karger.com/IAA


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