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Research Article

The GTPase Function of YvcJ and Its Subcellular Relocalization Are Dependent on Growth Conditions in Bacillus subtilis

Pompeo F. · Luciano J. · Brochier-Armanet C. · Galinier A.

Author affiliations

Laboratoire de Chimie Bactérienne, UPR 9043, IFR 88, CNRS, Aix-Marseille Université, Marseille, France

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J Mol Microbiol Biotechnol 2011;20:156–167

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Article / Publication Details

First-Page Preview
Abstract of Research Article

Published online: June 28, 2011
Issue release date: July 2011

Number of Print Pages: 12
Number of Figures: 6
Number of Tables: 2

ISSN: 1464-1801 (Print)
eISSN: 1660-2412 (Online)

For additional information: https://www.karger.com/MMB

Abstract

We have recently shown that the Bacillus subtilis GTPase YvcJ is involved in the phosphorylation of an unidentified cellular component and that the deletion of yvcJ induced a decrease in competence efficiency. In this paper, we report that growth conditions influence both the YvcJ-dependent phosphorylation event and the localization of this protein. More precisely, we have observed that YvcJ can be localized in the cell either as a helical-like pattern or as foci close to the poles and the septa depending on growth phase and on growth medium. In addition, we show that the mutation of the catalytic lysine residue (K22) located in the Walker A motif of YvcJ, and necessary for its GTPase activity, induces a decrease in competence efficiency similar to that observed for the yvcJ null mutant. This mutation also inhibits the YvcJ-dependent phosphorylation event. Furthermore, a phylogenetic analysis of the YvcJ homologues shows that this protein is ancient in Bacteria (being possibly present in their last common ancestor) and has been conserved in a number of major bacterial phyla, suggesting that this protein has an important function in this domain of life. To sum up, even if the precise cellular role of this ancient protein remains unknown, our data show that the GTPase activity of B. subtilis YvcJ and its function in the phosphorylation of a cellular component are influenced by the growth conditions, and are important for the effect of YvcJ on competence efficiency.

© 2011 S. Karger AG, Basel


References

  1. Altschul SF, Madden TL, Schaffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ: Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 1997;25:3389–3402.
  2. Barrick JE, Corbino KA, Winkler WC, Nahvi A, Mandal M, Collins J, Lee M, Roth A, Sudarsan N, Jona I, Wickiser JK, Breaker RR: New RNA motifs suggest an expanded scope for riboswitches in bacterial genetic control. Proc Natl Acad Sci USA 2004;101:6421–6426.
  3. Carballido-Lopez R: The bacterial actin-like cytoskeleton. Microbiol Mol Biol Rev 2006;70:888–909.
  4. Chen I, Dubnau D: DNA uptake during bacterial transformation. Nat Rev Microbiol 2004;2:241–249.
  5. Chen I, Provvedi R, Dubnau D: A macromolecular complex formed by a pilin-like protein in competent Bacillus subtilis. J Biol Chem 2006;281:21720–21727.
  6. Claessen D, Emmins R, Hamoen LW, Daniel RA, Errington J, Edwards DH: Control of the cell elongation-division cycle by shuttling of pbp1 protein in Bacillus subtilis. Mol Microbiol 2008;68:1029–1046.
  7. Defeu Soufo HJ, Reimold C, Linne U, Knust T, Gescher J, Graumann PL: Bacterial translation elongation factor EF-Tu interacts and colocalizes with actin-like MreB protein. Proc Natl Acad Sci USA 2010;107:3163–3168.
  8. Dubnau D: The regulation of genetic competence in Bacillus subtilis. Mol Microbiol 1991;5:11–18.
  9. Egger LA, Park H, Inouye M: Signal transduction via the histidyl-aspartyl phosphorelay. Genes Cells 1997;2:167–184.
  10. Foulquier E, Pompeo F, Bernadac A, Espinosa L, Galinier A: The YvcK protein is required for morphogenesis via localization of PBP1 under gluconeogenic growth conditions in Bacillus subtilis. Mol Microbiol 2011;80:309–318.
  11. Galinier A, Haiech J, Kilhoffer MC, Jaquinod M, Stülke J, Deutscher J, Martin-Verstraete I: The Bacillus subtilis crh gene encodes a HPr-like protein involved in carbon catabolite repression. Proc Natl Acad Sci USA 1997;94:8439–8444.
  12. Görke B, Foulquier E, Galinier A: YvcK of Bacillus subtilis is required for a normal cell shape and for growth on Krebs cycle intermediates and substrates of the pentose phosphate pathway. Microbiology 2005;151:3777–3791.
  13. Guindon S, Gascuel O: A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. Syst Biol 2003;52:696–704.
  14. Hahn J, Maier B, Haijema BJ, Sheetz M, Dubnau D: Transformation proteins and DNA uptake localize to the cell poles in Bacillus subtilis. Cell 2005;122:59–71.
  15. Hamoen LW, Venema G, Kuipers OP: Controlling competence in Bacillus subtilis: shared use of regulators. Microbiology 2003;149:9–17.
  16. Hudson KD, Corfe BM, Kemp EH, Feavers IM, Coote PJ, Moir A: Localization of geraa and gerac germination proteins in the Bacillus subtilis spore. J Bacteriol 2001;183:4317–4322.
  17. Jobb G, von Haeseler A, Strimmer K: Treefinder: A powerful graphical analysis environment for molecular phylogenetics. BMC Evol Biol 2004;4:18.
  18. Jones LJ, Carballido-Lopez R, Errington J: Control of cell shape in bacteria: helical, actin-like filaments in Bacillus subtilis. Cell 2001;104:913–922.
  19. Kalamorz F, Reichenbach B, Marz W, Rak B, Görke B: Feedback control of glucosamine-6-phosphate synthase GlmS expression depends on the small RNA GlmZ and involves the novel protein YhbJ in Escherichia coli. Mol Microbiol 2007;65:1518–1533.
  20. Kammann M, Laufs J, Schell J, Gronenborn B: Rapid insertional mutagenesis of DNA by polymerase chain reaction (PCR). Nucleic Acids Res 1989;17:5404.
  21. Katoh K, Toh H: Recent developments in the MAFFT multiple sequence alignment program. Brief Bioinform 2008;9:286–298.
  22. Kirstein J, Strahl H, Moliere N, Hamoen LW, Turgay K: Localization of general and regulatory proteolysis in Bacillus subtilis cells. Mol Microbiol 2008;70:682–694.
  23. Kobayashi K, Ehrlich SD, Albertini A, et al.: Essential Bacillus subtilis genes. Proc Natl Acad Sci USA 2003;100:4678–4683.
  24. Kunst F, Rapoport G: Salt stress is an environmental signal affecting degradative enzyme synthesis in Bacillus subtilis. J Bacteriol 1995;177:2403–2407.
  25. Le SQ, Gascuel O: An improved general amino acid replacement matrix. Mol Biol Evol 2008;25:1307–1320.
  26. Leipe DD, Koonin EV, Aravind L: Evolution and classification of P-loop kinases and related proteins. J Mol Biol 2003;333:781–815.
  27. Lewis PJ, Marston AL: Gfp vectors for controlled expression and dual labelling of protein fusions in Bacillus subtilis. Gene 1999;227:101–110.
  28. Luciano J, Foulquier E, Fantino JR, Galinier A, Pompeo F: Characterization of YvcJ, a conserved P-loop-containing protein, and its implication in competence in Bacillus subtilis. J Bacteriol 2009;191:1556–1564.
  29. Matte A, Tari LW, Delbaere LT: How do kinases transfer phosphoryl groups? Structure 1998;6:413–419.
  30. Michaelis AM, Gitai Z: Dynamic polar sequestration of excess MurG may regulate enzymatic function. J Bacteriol 2010;192:4597–4605.
  31. Moreno MS, Schneider BL, Maile RR, Weyler W, Saier MH Jr: Catabolite repression mediated by the ccpa protein in Bacillus subtilis: novel modes of regulation revealed by whole-genome analyses. Mol Microbiol 2001;39:1366–1381.
  32. Philippe H: MUST, a computer package of management utilities for sequences and trees. Nucleic Acids Res 1993;21:5264–5272.
  33. Pompeo F, Granet Y, Lavergne JP, Grangeasse C, Nessler S, Jault JM, Galinier A: Regulation and mutational analysis of the HPr kinase/phosphorylase from Bacillus subtilis. Biochemistry 2003;42:6762–6771.
  34. Poncet S, Mijakovic I, Nessler S, Gueguen-Chaignon V, Chaptal V, Galinier A, Boel G, Maze A, Deutscher J: HPr kinase/phosphorylase, a Walker motif A-containing bifunctional sensor enzyme controlling catabolite repression in Gram-positive bacteria. Biochim Biophys Acta 2004;1697:123–135.
  35. Ronquist F, Huelsenbeck JP: MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 2003;19:1572–1574.
  36. Sambrook J, Fritsch EF, Maniatis T: Molecular Cloning: A Laboratory Manual. Cold Spring Harbor, Cold Spring Harbor Laboratory Press, 1989.
  37. Saraste M, Sibbald PR, Wittinghofer A: The P-loop – A common motif in ATP- and GTP-binding proteins. Trends Biochem Sci 1990;15:430–434.
  38. Winkler WC, Nahvi A, Roth A, Collins JA, Breaker RR: Control of gene expression by a natural metabolite-responsive ribozyme. Nature 2004;428:281–286.

Article / Publication Details

First-Page Preview
Abstract of Research Article

Published online: June 28, 2011
Issue release date: July 2011

Number of Print Pages: 12
Number of Figures: 6
Number of Tables: 2

ISSN: 1464-1801 (Print)
eISSN: 1660-2412 (Online)

For additional information: https://www.karger.com/MMB


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