European Surgical Research
Original Paper
Direct Implantation of VX-2 Carcinoma: A New Rabbit Bone Model Using a Three-Dimensional Matrix as a Carrier for the Tumor CellsProschek D.a · Tonak M.a · Kafchitsas K.a · Zangos S.b · Mack M.G.b · Theisen A.c · Kurth A.A.aaCenter for Musculoskeletal Surgery, Department of Orthopaedic Surgery, University Medical Center, Mainz, Departments of bDiagnostic and Interventional Radiology and cAnimal Research, University Hospital Frankfurt, Frankfurt, Germany
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Article / Publication Details
Received: February 17, 2011
Accepted: June 15, 2011
Published online: September 27, 2011
Issue release date: October 2011
Number of Print Pages: 5
Number of Figures: 4
Number of Tables: 0
ISSN: 0014-312X (Print)
eISSN: 1421-9921 (Online)
For additional information: https://www.karger.com/ESR
Abstract
Background: Animal bone models are inevitable for musculoskeletal research. The induction of a local bone tumor is complex and time consuming. In this study a new model is presented using a direct implantation of tumor cells into the bone without a preliminary passaging of the cells. Methods: A three-dimensional matrix consisting of alginate spheroids and carrying the VX-2 tumor suspension was used for implantation into the bone of 6 female New Zealand white rabbits. X-ray imaging, CT and MRI scans as well as a histological examination were carried out. Results: All rabbits developed local bone tumor in the metaphysis of the femoral leg. Bone tumor was identifiable on average 6.2 weeks after implantation. Fluoroscopy, CT and MRI scans showed a cortical reaction but no destruction of the compact bone together with a mean tumor size of 14 mm. Histological examination revealed a tumor infiltration with an activation of osteoclasts and an osteoclastic resorption. Conclusion: The direct implantation of a VX-2 tumor suspension into the rabbit bone using alginate spheroids is an effective and reproducible way to successfully induce bone tumor. This new animal model allows further examination of surgical and minimal invasive therapy in musculoskeletal research.
© 2011 S. Karger AG, Basel
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Article / Publication Details
Received: February 17, 2011
Accepted: June 15, 2011
Published online: September 27, 2011
Issue release date: October 2011
Number of Print Pages: 5
Number of Figures: 4
Number of Tables: 0
ISSN: 0014-312X (Print)
eISSN: 1421-9921 (Online)
For additional information: https://www.karger.com/ESR
Copyright / Drug Dosage / Disclaimer
Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher.Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
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