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Original Paper

Phenotyping of Tianma-Stimulated Differentiated Rat Neuronal B104 Cells by Quantitative Proteomics

Sundaramurthi H.a · Manavalan A.a, b · Ramachandran U.a, b · Hu J.-M.c · Sze S.K.a · Heese K.a, b

Author affiliations

aSchool of Biological Sciences, and bInstitute of Advanced Studies, Nanyang Technological University, Singapore; cKunming Institute of Botany, Chinese Academy of Science, Kunming, China

Corresponding Author

Klaus Heese

School of Biological Sciences, Department of Molecular and Cell Biology Nanyang Technological University, 60 Nanyang Drive

Singapore 637551 (Singapore)

Tel. +65 6316 2848, E-Mail Klaus.Heese@rub.de

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Neurosignals 2012;20:48–60

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Gastrodia elata blume (tianma) is a traditional Chinese herb often used in the treatment of convulsions, headaches, and hypertension. Although interest in neuronal-related actions of tianma is increasing, minimal studies have been conducted to determine its specific effects on neuronal cells. This study was designed to examine the effects of tianma on the metabolism in differentiated neuroblastoma cells using the isobaric tag for relative and absolute quantitation (iTRAQ) technology. Stimulation of these cells with tianma caused changes in the expression of 38 proteins that were subsequently classified according to their physiological functions and association with neurodegenerative diseases. We identified six proteins with altered functional activities in neurodegenerative disease states that were modulated by tianma: triosephosphate isomerase (Tpi1), peptidyl-prolyl cis-trans isomerase A (Ppia), neural cell adhesion molecule 1 (Ncam1), ubiquitin carboxyl-terminal hydrolase isozyme L1 (Uchl1), septin-2 (Sept2) and heat shock protein 90 (Hsp90aa1). We postulate that tianma mediates its neuroprotective effects via upregulation of Ncam1, Hsp90aa1, Tpi1 and Ppia while downregulating Sept2 and Uchl1. These changes in protein expression aid in the restoration of the intracellular environment to a metabolically balanced state, promoting cell survival. Based on these observed data, we conclude that tianma has therapeutic potential, especially for neurodegenerative diseases.

© 2011 S. Karger AG, Basel


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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: May 25, 2011
Accepted: August 04, 2011
Published online: November 16, 2011
Issue release date: January 2012

Number of Print Pages: 13
Number of Figures: 8
Number of Tables: 1

ISSN: 1424-862X (Print)
eISSN: 1424-8638 (Online)

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