Improvement in Cardiac Function following Transplantation of Human Umbilical Cord Matrix-Derived Mesenchymal CellsLatifpour M.a, f · Nematollahi-Mahani S.N.a · Deilamy M.g · Azimzadeh B.S.d · Eftekhar-Vaghefi S.H.a · Nabipour F.b · Najafipour H.c · Nakhaee N.e · Yaghoubi M.h · Eftekhar-Vaghefi R.f · Salehinejad P.j · Azizi H.i
aDepartment of Anatomy, bDepartment of Pathology, cDepartment of Physiology and Physiology Research Center, dCardiovascular Division, Shafa Hospital, Afzalipour School of Medicine, eNeuroscience Research Center, and fStudents Research Center, Kerman University of Medical Sciences, gDepartment of Cardiovascular Surgery, Al-Zahra Hospital, hDepartment of Biotechnology, Research Institute of Environmental Science, International Center for Science, High Technology and Environmental Science, Kerman, and iDepartment of Stem Cells, Cell Science Research Center, Royan Institute, Tehran, Iran; jInstitute of Bioscience, University Putra Malaysia, Kuala Lumpur, Malaysia
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Article / Publication Details
Objectives: Human umbilical cord mesenchymal cells (hUCM) can be easily obtained and processed in a laboratory. These cells may be considered as a suitable source in the repair of heart failure diseases. We, therefore, examined whether these cells may contribute to heart regeneration following an acute experimental myocardial infarction (MI). Methods: MI-induced animals received 5 × 106 hUCM cells, 5 × 106 5-azacytidine-treated cells (dhUCM), or PBS alone, subepicardially. A group of animals with MI and no other former intervention served as controls. dhUCM cells were assessed for F-actin, myogenin and troponin-I expression. Results: dhUCM cells appeared as binucleated cells with extensive cytoplasmic processes. These differentiated cells were F-actin and myogenin positive. Thirty days after LAD ligation, left ventricular ejection fraction and the percentage of fractional shortening improved significantly in cell-receiving animals. In addition, the amount of scar tissue was significantly reduced in hUCM and dhUCM groups compared to MI group (p < 0.05). These parameters were comparable between hUCM and dhUCM groups. Histopathological evaluations revealed that some engrafted cells adjacent to and remote from the MI area expressed troponin-I, F-actin and connexin43. Conclusion: These findings demonstrated the potential therapeutic use of either differentiated or undifferentiated hUCM cells in treatment of heart failure conditions.
© 2011 S. Karger AG, Basel
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