Cytogenetic and Genome Research

Original Article

A FISH Karyotype to Study Chromosome Polymorphisms for the Elytrigia elongata E Genome

Linc G. · Sepsi A. · Molnár-Láng M.

Author affiliations

Agricultural Research Institute of the Hungarian Academy of Sciences, Martonvásár, Hungary

Keywords: mcFISH karyotypepSc119.218S-5.8S-26S rDNAAfa familyChromosome polymorphismElytrigia elongata

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Cytogenet Genome Res 2012;136:138–144
https://doi.org/10.1159/000334835

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Article / Publication Details

First-Page Preview
Abstract of Original Article

Accepted: September 26, 2011
Published online: December 20, 2011
Issue release date: March 2012

Number of Print Pages: 7
Number of Figures: 3
Number of Tables: 0

ISSN: 1424-8581 (Print)
eISSN: 1424-859X (Online)

For additional information: https://www.karger.com/CGR

Abstract

Elytrigia elongata (Host) Nevski(= Agropyron elongatum, Thinopyrum elongatum, 2n = 2x = 14, EE) has long been used as a source of various types of resistance for wheat improvement, and numerous transfers have been made. However, despite heavy use, no high-resolution karyotype exists. We characterized the E. elongata karyotype of several accessions applying highly repetitive DNA sequences as mcFISH probes for chromosome identification. The complete E. elongata disomic chromosome addition series and 11 ditelosomic addition lines in Chinese Spring wheat were exposed to sequential GISH-mcFISH. Based on the mcFISH results, each complete chromosome and each telocentric studied was unambiguously identified. The validation of the karyotype in 4 E. elongata accessions with different geographical origins showed extensive variations in the probe hybridization patterns, but this did not prevent chromosome identification. The established karyotype will be useful for the rapid identification of potential donor chromosomes in wheat improvement programs, allowing appropriate alien transfer.

© 2011 S. Karger AG, Basel



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Article / Publication Details

First-Page Preview
Abstract of Original Article

Accepted: September 26, 2011
Published online: December 20, 2011
Issue release date: March 2012

Number of Print Pages: 7
Number of Figures: 3
Number of Tables: 0

ISSN: 1424-8581 (Print)
eISSN: 1424-859X (Online)

For additional information: https://www.karger.com/CGR

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