Regulation of Phosphatidylserine Exposure in Red Blood CellsNguyen D.B.1,2 · Wagner-Britz L.1 · Maia S.1 · Steffen P.3 · Wagner C.3 · Kaestner L.4 · Bernhardt I.1
1Laboratory of Biophysics, Faculty of Natural and Technical Sciences III, Saarland University, Building A2 4, Saarbruecken,2Department of Molecular Biology, Faculty of Biotechnology, Hanoi University of Agriculture, Ngo Xuan Quang, Gia Lam, Hanoi,3Department of Experimental Physics, Saarland University, Building E2 6, Saarbruecken,4Institute for Molecular Cell Biology, School of Medicine, Saarland University, Building 61, Homburg
Universität des Saarlandes, Naturwiss.-Techn. Fakultät III
Zentrales Isotopenlabor / AG Biophysik, Gebäude A2.4
P.O.Box 151150, 66041 Saarbrücken (Germany)
Tel. +49 681 3026689, E-Mail firstname.lastname@example.org
Do you have an account?
The exposure of phosphatidylserine (PS) on the outer membrane leaflet of red blood cells (RBCs) serves as a signal for eryptosis, a mechanism for the RBC clearance from blood circulation. The process of PS exposure was investigated as function of the intracellular Ca2+ content and the activation of PKCα in human and sheep RBCs. Cells were treated with lysophosphatidic acid (LPA), 4-bromo-A23187, or phorbol-12 myristate-13 acetate (PMA) and analysed by flow cytometry, single cell fluorescence video imaging, or confocal microscopy. For human RBCs, no clear correlation existed between the number of cells with an elevated Ca2+ content and PS exposure. Results are explained by three different mechanisms responsible for the PS exposure in human RBCs: (i) Ca2+-stimulated scramblase activation (and flippase inhibition) by LPA, 4-bromo-A23187, and PMA; (ii) PKC activation by LPA and PMA; and (iii) enhanced lipid flop caused by LPA. In sheep RBCs, only the latter mechanism occurs suggesting absence of scramblase activity.
© 2011 S. Karger AG, Basel
Article / Publication Details
Copyright / Drug Dosage / DisclaimerCopyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher.
Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.