Login to MyKarger

New to MyKarger? Click here to sign up.



Login with Facebook

Forgot your password?

Authors, Editors, Reviewers

For Manuscript Submission, Check or Review Login please go to Submission Websites List.

Submission Websites List

Institutional Login
(Shibboleth or Open Athens)

For the academic login, please select your country in the dropdown list. You will be redirected to verify your credentials.

Original

Free Access

Involvement of Na+-leak Channel in Substance P-induced Depolarization of Pacemaking Activity in Interstitial Cells of Cajal

Kim B.J.1 · Chang I.Y.2 · Choi S.3 · Jun J.Y.3 · Jeon J.-H.4 · Xu W.-X.5 · Kwon Y.K.1 · Ren D.6 · So I.4

Author affiliations

1Division of Longevity and Biofunctional Medicine, Pusan National University School of Korean Medicine, Yangsan;2Department of Anatomy, Chosun University College of Medicine, Gwangju;3Department of Physiology, Chosun University College of Medicine, Gwangju;4Department of Physiology, Seoul National University College of Medicine, Seoul;5Department of Physiology, Shanghai Jiaotong University School of Medicine, Shanghai;6Department of Biology, University of Pennsylvania, Philadelphia, PA

Corresponding Author

Insuk So

Department of Physiology

Seoul National University College of Medicine

Yongeon-dong, Chongro-gu, Seoul (Korea)

E-Mail insuk@snu.ac.kr or Byung Joo Kim E-Mail vision@pusan.ac.kr

Related Articles for ""

Cell Physiol Biochem 2012;29:501–510

Do you have an account?

Login Information





Contact Information










I have read the Karger Terms and Conditions and agree.



Abstract

Interstitial cells of Cajal (ICCs) are the pacemaking cells in the gastrointestinal muscles that generate the rhythmic oscillations in membrane potential known as slow waves. ICCs also mediate or transduce inputs from the enteric nervous system. Substance P (SubP) is a member of the family of mammalian tachykinin peptides that are predominantly released by enteric neurons. This study assessed the relationship of Na+-leak channel (NALCN) in the SubP-induced depolarization in pacemaking activity in the gastrointestinal tract. The patch-clamp technique for whole-cell recording was used in cultured cluster and single ICCs. Electrophysiological and pharmacological properties of SubP in ICC pacemaking activity were similar to those of NALCN. Reverse-transcription polymerase chain reaction, Western blotting, and immunohistochemistry all showed abundant and localized expression of NALCN messenger RNA and protein in mouse small intestine. NALCN is involved in the SubP-induced depolarization of intestinal pacemaking activity. The protein is a potential target for pharmacological treatment of motor disorders of the gut.

© 2012 S. Karger AG, Basel


Article / Publication Details

First-Page Preview
Abstract of Original

Accepted: February 07, 2012
Published online: April 03, 2012
Issue release date: April 2012

Number of Print Pages: 10
Number of Figures: 1
Number of Tables: 0

ISSN: 1015-8987 (Print)
eISSN: 1421-9778 (Online)

For additional information: https://www.karger.com/CPB


Copyright / Drug Dosage / Disclaimer

Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher.
Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.