Involvement of Na+-leak Channel in Substance P-induced Depolarization of Pacemaking Activity in Interstitial Cells of CajalKim B.J.1 · Chang I.Y.2 · Choi S.3 · Jun J.Y.3 · Jeon J.-H.4 · Xu W.-X.5 · Kwon Y.K.1 · Ren D.6 · So I.4
1Division of Longevity and Biofunctional Medicine, Pusan National University School of Korean Medicine, Yangsan;2Department of Anatomy, Chosun University College of Medicine, Gwangju;3Department of Physiology, Chosun University College of Medicine, Gwangju;4Department of Physiology, Seoul National University College of Medicine, Seoul;5Department of Physiology, Shanghai Jiaotong University School of Medicine, Shanghai;6Department of Biology, University of Pennsylvania, Philadelphia, PA
Department of Physiology
Seoul National University College of Medicine
Yongeon-dong, Chongro-gu, Seoul (Korea)
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Interstitial cells of Cajal (ICCs) are the pacemaking cells in the gastrointestinal muscles that generate the rhythmic oscillations in membrane potential known as slow waves. ICCs also mediate or transduce inputs from the enteric nervous system. Substance P (SubP) is a member of the family of mammalian tachykinin peptides that are predominantly released by enteric neurons. This study assessed the relationship of Na+-leak channel (NALCN) in the SubP-induced depolarization in pacemaking activity in the gastrointestinal tract. The patch-clamp technique for whole-cell recording was used in cultured cluster and single ICCs. Electrophysiological and pharmacological properties of SubP in ICC pacemaking activity were similar to those of NALCN. Reverse-transcription polymerase chain reaction, Western blotting, and immunohistochemistry all showed abundant and localized expression of NALCN messenger RNA and protein in mouse small intestine. NALCN is involved in the SubP-induced depolarization of intestinal pacemaking activity. The protein is a potential target for pharmacological treatment of motor disorders of the gut.
© 2012 S. Karger AG, Basel
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