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Original Paper

Differential Response of MC3T3-E1 and Human Mesenchymal Stem Cells to Inositol Hexakisphosphate

Arriero M. del M.a · Ramis J.M.a · Perelló J.b · Monjo M.a

Author affiliations

aDepartment of Fundamental Biology and Health Sciences, Research Institute on Health Sciences (IUNICS), University of Balearic Islands; bLaboratoris Sanifit, ParcBIT. Building Disset, office D3. Ctra Valldemossa km 7.4. E-07121 Palma de Mallorca

Corresponding Author

Marta Monjo, PhD

Department of Fundamental Biology and Health Sciences,

Research Institute on Health Sciences (IUNICS), University of Balearic Islands.

Cra. de Valldemossa, km 7.5 (E-07122). Palma de Mallorca (Spain)

Tel. +34 971 259960, Fax +34 971 173184, E-Mail marta.monjo@uib.es

Related Articles for ""

Cell Physiol Biochem 2012;30:974-986

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Abstract

Background: Inositol hexakisphosphate (IP6) has been found to have an important role in biomineralization. Methods: Because the complete mechanism of action of IP6 on osteoblasts is not fully understood and its potential use in the primary prevention of osteoporosis, we examined the direct effect of IP6 on cell viability and differentiation of MC3T3-E1 cells and on differentiation of human umbilical cord mesenchymal stem cells (hUC-MSCs). Results: We show that IP6 has different effects depending on the origin of the cell target. Thus, while IP6 decreased gene expression of osteoblast markers and mineralization in MC3T3-E1 cells without negatively affecting cell viability and ALP activity, an increase in gene expression of ALP was observed in hUC-MSCs committed to the osteoblastic lineage. This increasing effect of IP6 on ALP mRNA expression levels was reversed by the addition of a selective inhibitor of IP6 kinase, suggesting that the effect of IP6 might be due through its pyrophosphorylated derivatives. Besides, Rankl mRNA levels were decreased after IP6 treatment in MC3T3-E1 cells, pointing to a paracrine effect on osteoclasts. Conclusion: Our results indicate that IP6 has different effects on osteoblast differentiation depending on the cell type and origin. However, further studies are needed to examine the net effect of IP6 on bone formation and its potential as novel antiosteoporosis drug.

© 2012 S. Karger AG, Basel


Article / Publication Details

First-Page Preview
Abstract of Original Paper

Accepted: August 29, 2012
Published online: September 20, 2012
Issue release date: September 2012

Number of Print Pages: 13
Number of Figures: 0
Number of Tables: 0

ISSN: 1015-8987 (Print)
eISSN: 1421-9778 (Online)

For additional information: http://www.karger.com/CPB


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