Cytogenetic and Genome Research

Original Article

Cytogenetic Analysis of Lagria villosa (Coleoptera, Tenebrionidae): Emphasis on the Mechanism of Association of the Xyp Sex Chromosomes

Goll L.G.a · Artoni R.F.a · Vicari M.R.a · Nogaroto V.a · Petitpierre E.b · Almeida M.C.a

Author affiliations

aUEPG, Setor de Ciências Biológicas e da Saúde, Departamento de Biologia Estrutural, Molecular e Genética, Ponta Grossa, Brazil; bLaboratory of Genetics, Department of Biology, University of the Balearic Islands, Palma de Mallorca, Spain

Keywords: FISHHeterochromatinKaryotypeMeiosisSynapseSynaptonemal complex

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Cytogenet Genome Res 2013;139:29–35
https://doi.org/10.1159/000341674

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Article / Publication Details

First-Page Preview
Abstract of Original Article

Accepted: June 20, 2012
Published online: August 30, 2012
Issue release date: December 2012

Number of Print Pages: 7
Number of Figures: 4
Number of Tables: 0

ISSN: 1424-8581 (Print)
eISSN: 1424-859X (Online)

For additional information: https://www.karger.com/CGR

Abstract

The Xyp sex determination mechanism is the system most frequent and ancestral to Coleoptera. Moreover, the presence of argyrophilous material associated with the sex bivalent is described as being responsible for the maintenance and association of these chromosomes. There are no karyotype data available regarding the genus Lagria and no consensus in the literature regarding the argyrophilous material present in the lumen of sex bivalent. Therefore, the aim of this work was to investigate the mechanism of sex chromosome bivalent association in Lagria villosa by analyzing the argyrophilous nature of the material present in the Xyp lumen. It was also intended to characterize L. villosa cytogenetically. The analysis of meiotic cells showed 2n = 18 = 16+Xyp for males and 2n = 18 = 16+XX in females and the meiotic formula was 2n = 8II+Xyp. The C-banding showed blocks of pericentromeric heterochromatin in all chromosomes except in the yp chromosome. In these regions, the use of fluorochromes revealed the presence of heterochromatin containing GC rich DNA sequences. The study of synaptonemal complex showed a gradual increase in the electron-density of the axial elements of the sex chromosomes and their association with strongly electron-dense material. The pepsin pretreatment revealed that the material impregnated by silver is protein.

© 2012 S. Karger AG, Basel



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Article / Publication Details

First-Page Preview
Abstract of Original Article

Accepted: June 20, 2012
Published online: August 30, 2012
Issue release date: December 2012

Number of Print Pages: 7
Number of Figures: 4
Number of Tables: 0

ISSN: 1424-8581 (Print)
eISSN: 1424-859X (Online)

For additional information: https://www.karger.com/CGR

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2013, Vol.139, No. 1

December 2012

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