Acta Haematologica

Original Paper

Genetic and Immunohistochemical Characterization of Epstein-Barr Virus-Associated Diffuse Large B-Cell Lymphoma

Al-Humood S. · AlQallaf A. · Al-Shemmari S. · Al-Faris L. · Al-Ayadhy B.

Author affiliations

Department of Pathology, Faculty of Medicine, Kuwait University, Safat, Kuwait

Keywords: BCL6Comparative genomic hybridizationDiffuse large B-cell lymphomaEpstein-Barr virus

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Acta Haematol 2014;131:1-10
https://doi.org/10.1159/000350493

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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: July 25, 2012
Accepted: February 28, 2013
Published online: September 04, 2013
Issue release date: December 2013

Number of Print Pages: 10
Number of Figures: 5
Number of Tables: 5

ISSN: 0001-5792 (Print)
eISSN: 1421-9662 (Online)

For additional information: https://www.karger.com/AHA

Abstract

Epstein-Barr virus (EBV) has a pathogenic role in several lymphomas, including diffuse large B-cell lymphoma (DLBCL). EBV-associated genetic aberrations in DLBCL have not been fully characterized. The aim of this study was to investigate the prevalence of EBV infection in sporadic DLBCL cases in Kuwait and to evaluate their EBV status in relation to demographic data, the anatomical disease site, immunophenotypic features, particularly pertaining to the Choi's DLBCL prognostic classification, and chromosomal aberrations. Using immunohistochemistry (IHC), in situ hybridization (ISH), nested polymerase chain reaction (nPCR) and comparative genomic hybridization techniques, formalin-fixed paraffin-embedded blocks of archived DLBCL cases were included and evaluated in the study. EBV was detected in 6.9, 18.2 and 25% of the studied cases using IHC, ISH and nPCR, respectively, indicating that nPCR is more sensitive in detecting EBV than IHC and ISH. EBV- DLBCL cases showed BCL6 protein expression more frequently than EBV+ DLBCL cases. The reported prevalence of EBV+ DLBCL cases in this study is similar to that reported in the literature using ISH results and higher using nPCR results. There was a significant inverse correlation between BCL6 protein expression and the presence of EBV (p = 0.01).

© 2013 S. Karger AG, Basel



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References

  1. Choi WW, Weisenburger DD, Greiner TC, Piris MA, Banham AH, Delabie J, Braziel RM, Geng H, Iqbal J, Lenz G, Vose JM, Hans CP, Fu K, Smith LM, Li M, Liu Z, Gascoyne RD, Rosenwald A, Ott G, Rimsza LM, Campo E, Jaffe ES, Jaye DL, Staudt LM, Chan WC: A new immunostain algorithm classifies diffuse large B-cell lymphoma into molecular subtypes with high accuracy. Clin Cancer Res 2009;15:5494-5502.
    External Resources
  2. De Paepe P, De Wolf-Peeters C: Diffuse large B-cell lymphoma: a heterogeneous group of non-Hodgkin's lymphomas comprising several distinct clinicopathological entities. Leukemia 2007;21:37-43.
    External Resources
  3. Alizadeh AA, Eisen MB, Davis RE, Ma C, Lossos IS, Rosenwald A, Boldrick JC, Sabet H, Tran T, Yu X, Powell JI, Yang L, Marti GE, Moore T, Hudson J Jr, Lu L, Lewis DB, Tibshirani R, Sherlock G, Chan WC, Greiner TC, Weisenburger DD, Armitage JO, Warnke R, Levy R, Wilson W, Grever MR, Byrd JC, Botstein D, Brown PO, Staudt LM: Distinct types of diffuse large B-cell lymphoma identified by gene expression profiling. Nature 2000;403:503-511.
    External Resources
  4. Rosenwald A, Wright G, Chan WC, Connors JM, Campo E, Fisher RI, Gascoyne RD, Muller-Hermelink HK, Smeland EB, Giltnane JM, Hurt EM, Zhao H, Averett L, Yang L, Wilson WH, Jaffe ES, Simon R, Klausner RD, Powell J, Duffey PL, Longo DL, Greiner TC, Weisenburger DD, Sanger WG, Dave BJ, Lynch JC, Vose J, Armitage JO, Montserrat E, López-Guillermo A, Grogan TM, Miller TP, LeBlanc M, Ott G, Kvaloy S, Delabie J, Holte H, Krajci P, Stokke T, Staudt LM; Lymphoma/Leukemia Molecular Profiling Project: the use of molecular profiling to predict survival after chemotherapy for diffuse large-B-cell lymphoma for the Lymphoma/Leukemia Molecular Profiling Project. N Engl J Med 2002;346:1937-1947.
    External Resources
  5. Hans CP, Weisenburger DD, Greiner TC, Gascoyne RD, Delabie J, Ott G, Müller-Hermelink HK, Campo E, Braziel RM, Jaffe ES, Pan Z, Farinha P, Smith LM, Falini B, Banham AH, Rosenwald A, Staudt LM, Connors JM, Armitage JO, Chan WC: Confirmation of the molecular classification of diffuse large B-cell lymphoma by immunohistochemistry using a tissue microarray. Blood 2004;103:275-282.
    External Resources
  6. Poulsen CB, Borup R, Nielsen FC, Borregaard N, Hansen M, Grønbaek K, Møller MB, Ralfkiaer E: Microarray-based classification of diffuse large B-cell lymphoma. Eur J Haematol 2005;74:453-465.
    External Resources
  7. Oschlies I, Klapper W, Zimmermann M, Krams M, Wacker HH, Burkhardt B, Harder L, Siebert R, Reiter A, Parwaresch R: Diffuse large B-cell lymphoma in pediatric patients belongs predominantly to the germinal-center type B-cell lymphomas: a clinicopathologic analysis of cases included in the German BFM (Berlin-Frankfurt-Münster) Multicenter Trial. Blood 2006;107:4047-4052.
    External Resources
  8. Armitage JO, Weisenburger DD: New approach to classifying non-Hodgkin's lymphomas: clinical features of the major histopathologic subtypes. Non-Hodgkin's Lymphoma Classification Project. J Clin Oncol 1998;16:2780-2795.
    External Resources
  9. Cancer Registry. Kuwait Cancer Control Center. Kuwait, Kuwait Cancer Registry Department, 2009.
  10. Carbone A, Gloghini A, Dotti G: EBV-associated lymphoproliferative disorders: classification and treatment. Oncologist 2008;13:577-585.
    External Resources
  11. Park S, Lee J, Ko YH, Han A, Jun HJ, Lee SC, Hwang IG, Park YH, Ahn JS, Jung CW, Kim K, Ahn YC, Kang WK, Park K, Kim WS: The impact of Epstein-Barr virus status on clinical outcome in diffuse large B-cell lymphoma. Blood 2007;110:972-978.
    External Resources
  12. Jarrett RF, Stark GL, White J, Angus B, Alexander FE, Krajewski AS, Freeland J, Taylor GM, Taylor PR, Scotland and Newcastle Epidemiology of Hodgkin Disease Study Group: Impact of tumor Epstein-Barr virus status on presenting features and outcome in age-defined subgroups of patients with classic Hodgkin lymphoma: a population-based study. Blood 2005;106:2444-2451.
    External Resources
  13. Hummel M, Anagnostopoulos I, Korbjuhn P, Stein H: Epstein-Barr virus in B-cell non-Hodgkin's lymphomas: unexpected infection patterns and different infection incidence in low- and high-grade types. J Pathol 1995;175:263-271.
    External Resources
  14. Kapatai G, Murray P: Contribution of the Epstein Barr virus to the molecular pathogenesis of Hodgkin lymphoma. J Clin Pathol 2007;60:1342-1349.
    External Resources
  15. Dolcetti R, Masucci MG: Epstein-Barr virus: induction and control of cell transformation. J Cell Physiol 2003;196:207-218.
    External Resources
  16. Umemoto M, Yokoyama Y, Sato S, Tsuchida S, Al-Mulla F, Saito Y: Carbonyl reductase as a significant predictor of survival and lymph node metastasis in epithelial ovarian cancer. Br J Cancer 2001;85:1032-1036.
    External Resources
  17. Al-Mulla F, Behbehani AI, Bitar MS, Varadharaj G, Going JJ: Genetic profiling of stage I and II colorectal cancer may predict metastatic relapse. Mod Pathol 2006;19:648-658.
    External Resources
  18. Kuze T, Nakamura N, Hashimoto Y, Sasaki Y, Abe M: The characteristics of Epstein-Barr virus (EBV)-positive diffuse large B-cell lymphoma: comparison between EBV+ and EBV- cases in Japanese population. Jpn J Cancer Res 2000;91:1233-1240.
    External Resources
  19. Heslop HE: Biology and treatment of Epstein-Barr virus-associated non-Hodgkin's lymphomas. Hematology Am Soc Hematol Educ Program 2005, pp 260-266.
  20. d'Amore F, Johansen P, Houmand A, Weisenburger DD, Mortensen LS: Epstein-Barr virus genome in non-Hodgkin's lymphoma occurring in immunocompetent patients: highest prevalence in nonlymphoblastic T-cell lymphoma and correlation with a poor prognosis. Blood 1996;87:1045-1055.
    External Resources
  21. Oyama T, Ichimura K, Suzuki R, Suzumiya J, Ohshima K, Yatabe Y, Yokoi T, Kojima M, Kamiya Y, Taji H, Kagami Y, Ogura M, Saito H, Morishima Y, Nakamura S: Senile EBV+ B-cell lymphoproliferative disorders: a clinicopathologic study of 22 patients. Am J Surg Pathol 2003;27:16-26.
    External Resources
  22. Ohshima K, Suzumiya J, Tasiro K, Mukai Y, Tanaka T, Kato A, Kikuchi M: Epstein-Barr virus infection and associated products (LMP, EBNA2, vIL-10) in nodal non-Hodgkin's lymphoma of human immunodeficiency virus-negative Japanese. Am J Hematol 1996;52:21-28.
    External Resources
  23. Al-Humood SA, Al-Qallaf AS, Alshemmari SH, Francis IM, Junaid TA, Marouf RA, Al-Mulla F: Genotypic and phenotypic differences between nodal and extranodal diffuse large B-cell lymphomas. J Histochem Cytochem 2011;59:918-931.
    External Resources
  24. Yamaguchi M, Seto M, Okamoto M, Ichinohasama R, Nakamura N, Yoshino T, Suzumiya J, Murase T, Miura I, Akasaka T, Tamaru J, Suzuki R, Kagami Y, Hirano M, Morishima Y, Ueda R, Shiku H, Nakamura S: De novo CD5+ diffuse large B-cell lymphoma: a clinicopathologic study of 109 patients. Blood 2002;99:815-821.
    External Resources
  25. Sjö LD, Poulsen CB, Hansen M, Møller MB, Ralfkiaer E: Profiling of diffuse large B-cell lymphoma by immunohistochemistry: identification of prognostic subgroups. Eur J Haematol 2007;79:501-507.
    External Resources
  26. Berglund M, Thunberg U, Amini R, Book M, Roos G, Erlanson M, Linderoth J, Dictor M, Jerkeman M, Cavallin-Ståhl E, Sundström C, Rehn-Eriksson S, Backlin C, Hagberg H, Rosenquist R, Enblad G: Evaluation of immunophenotype in diffuse large B-cell lymphoma and its impact on prognosis. Mod Pathol 2005;18:1113-1120.
    External Resources
  27. Hasselblom S, Ridell B, Nilsson-Ehle H, Andersson PO: The impact of gender, age and patient selection on prognosis and outcome in diffuse large B-cell lymphoma - a population-based study. Leuk Lymphoma 2007;48:736-745.
    External Resources
  28. Shimoyama Y, Oyama T, Asano N, Oshiro A, Suzuki R, Kagami Y, Morishima Y, Nakamura S: Senile Epstein-Barr virus-associated B-cell lymphoproliferative disorders: a mini review. J Clin Exp Hematop 2006;46:1-4.
    External Resources
  29. Boccellato F, Anastasiadou E, Rosato P, Kempkes B, Frati L, Faggioni A, Trivedi P: EBNA2 interferes with the germinal center phenotype by down regulating BCL6 and TCL1 in non-Hodgkin's lymphoma cells. J Virol 2007;81:2274-2282.
    External Resources
  30. Kim LH, Eow GI, Peh SC, Poppema S: The role of CD30, CD40 and CD95 in the regulation of proliferation and apoptosis in classical Hodgkin's lymphoma. Pathology 2003;35:428-435.
    External Resources
  31. Vaghefi P, Martin A, Prévot S, Charlotte F, Camilleri-Broët S, Barli E, Davi F, Gabarre J, Raphael M, Poirel HA: Genomic imbalances in AIDS-related lymphomas: relation with tumoral Epstein-Barr virus status. AIDS 2006;20:2285-2291.
    External Resources
  32. Kamranvar SA, Gruhne B, Szeles A, Masucci MG: Epstein-Barr virus promotes genomic instability in Burkitt's lymphoma. Oncogene 2007;26:5115-5123.
    External Resources

Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: July 25, 2012
Accepted: February 28, 2013
Published online: September 04, 2013
Issue release date: December 2013

Number of Print Pages: 10
Number of Figures: 5
Number of Tables: 5

ISSN: 0001-5792 (Print)
eISSN: 1421-9662 (Online)

For additional information: https://www.karger.com/AHA

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