Development of an Animal Model to Evaluate the Allergenicity of Food AllergensAhrens B.a · Quarcoo D.a, c · Buhner S.b, d · Reese G.f · Vieths S.f · Hamelmann E.a, e
Departments of aPediatric Pneumology and Immunology, bGastroenterology and cInstitute of Occupational Medicine, Charité Universitätsmedizin, Berlin, dHuman Biology Technische Universität, Munich, eUniversity Children's Hospital, Ruhr University, Bochum, and fPaul-Ehrlich-Institut, Division of Allergology, Langen, Germany
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Scope: Considering the increasing numbers of patients suffering from food allergy (FA) as well as the great variety of novel foods and food compositions, an unmet need exists for the development of preclinical approaches to characterize the allergenic potential of proteins. The aim of our study was to evaluate the allergenicity of different food allergens in a rat model. Methods: Brown Norway rats were sensitized to protein extracts (RuBisCO, apple, soy, peanut, garden pea) or ovalbumin (OVA) combined with Bordetella pertussis and aluminium hydroxide, followed by oral allergen challenges. Results: Allergen-specific serum immunoglobulin production and the proliferation of mononuclear cells from spleen confirmed sensitization. To assess functional alterations in the gut, intestinal permeability was measured, which increased in sensitized and challenged animals compared to non-sensitized controls. Allergens with high allergenic potential (peanut, OVA, soy) caused a stronger immunological response than allergens with low allergenic potential, such as RuBisCO and apple. Moreover, the immunological responses were reduced when using boiled instead of raw soy and pea proteins. Conclusion: This model mimics key features of FA and facilitates investigating the allergenicity of allergens in novel food or food compositions in vivo.
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- Sicherer SH, Sampson HA: Food allergy: epidemiology, pathogenesis, diagnosis, and treatment. J Allergy Clin Immunol 2014;133:291-307.
- Radauer C, Bublin M, Wagner S, Mari A, Breiteneder H: Allergens are distributed into few protein families and possess a restricted number of biochemical functions. J Allergy Clin Immunol 2008;121:847-852.
- Rona RJ, Keil T, Summers C, Gislason D, Zuidmeer L, Sodergren E, Sigurdardottir ST, Lindner T, Goldhahn K, Dahlstrom J, McBride D, Madsen C: The prevalence of food allergy: a meta-analysis. J Allergy Clin Immunol 2007;120:638-646.
- Pumphrey RS: Lessons for management of anaphylaxis from a study of fatal reactions. Clin Exp Allergy 2000;30:1144-1150.
- Matricardi PM, Bockelbrink A, Beyer K, et al: Primary versus secondary immunoglobulin E sensitization to soy and wheat in the Multi-Centre Allergy Study cohort. Clin Exp Allergy 2008;38:493-500.
- Savage JH, Kaeding AJ, Matsui EC, Wood RA: The natural history of soy allergy. J Allergy Clin Immunol 2010;125:683-686.
- De Swert LF, Gadisseur R, Sjölander S, Raes M, Leus J, Van Hoeyveld E: Secondary soy allergy in children with birch pollen allergy may cause both chronic and acute symptoms. Pediatr Allergy Immunol 2012;23:117-123.
- Beyer K, Morrow E, Li XM, Bardina L, Bannon GA, Burks AW, Sampson HA: Effects of cooking methods on peanut allergenicity. J Allergy Clin Immunol 2001;107:1077-1081.
- Lehrer SB, McClain S: Utility of animal models for predicting human allergenicity. Regul Toxicol Pharmacol 2009;54(suppl 3):S46-S51.
- Peeters KABM, Koppelman SJ, Penninks AH, Lebens A, Bruijnzeel-Koomen CAFM, Hefle SL, Taylor SL, van Hoffen E, Knulst AC: Clinical relevance of sensitization to lupine in peanut-sensitized adults. Allergy 2009;64:549-555.
Food and Agriculture Organization of the United Nations/World Health Organization (FAO/WHO): Report of a Joint FAO/WHO Expert Consultation on Allergenicity of Foods Derived from Biotechnology. Rome, Food and Agriculture Organization of the United Nations, 2001.
Food and Agriculture Organization of the United Nations/World Health Organization (FAO/WHO): Codex Alimentarius Guideline for Conduct of Food Safety Assessments of Foods Derived from Recombinant-DNA Plants; Appendix IV: Annex on Assessment of Possible Allergenicity. Geneva, Codex Alimentarius Commission, 2003, pp 47-60.
EFSA Panel on Genetically Modified Organisms: Guidance for risk assessment of food and feed from genetically modified plants. EFSA J 2011;9:2150. http://www.efsa.europa.eu/en/efsajournal/pub/2150.htm.
Bucchini L, Goldman LR: Starlink corn: a risk analysis. Environ Health Perspect 2002;110:5-13.
- Finkleman FD: Anaphylaxis: lessons from mouse models. J Allergy Clin Immunol 2007;120:506-515.
- Lemon-Mule H, Nowak-Wegryzn A, Berin C, Knight AK: Pathophysiology of food-induced anaphylaxis. Curr Allergy Asthma Rep 2008;8:201-208.
- Knippels LM, Penninks AH, Smit JJ, Houben GF: Immune-mediated effects upon oral challenge of ovalbumin-sensitized Brown Norway rats: further characterization of a rat food allergy model. Toxicol Appl Pharmacol 1999;156:161-169.
- Ahrens B, Quarcoo D, Buhner S, Matricardi PM, Hamelmann E: Oral administration of bacterial lysates attenuates experimental food allergy. Int Arch Allergy Immunol 2011;156:196-204.
- Vieths S, Hoffmann A, Holzhauser T, Müller U, Reindl J, Haustein D: Factors influencing the quality of food extracts for in vitro and in vivo diagnosis. Allergy 1998;53(suppl):65-71.
- Hirano T, Yamakawa N, Miyajima H, Maeda K, Takai S, Ueda A, Taniguchi O, Hashimoto H, Hirose S, Okumura K, et al: An improved method for the detection of IgE antibody of defined specificity by ELISA using rat monoclonal anti-IgE antibody. J Immunol Methods 1989;119:145-150.
- Buhner S, Buning C, Genschel J, Kling K, Herrmann D, Dignass A, Kuechler I, Krueger S, Schmidt HH-J, Lochs H: Genetic basis for increased intestinal permeability in families with Crohn's disease: role of CARD15 3020insC mutation? Gut 2006;55:342-347.
- Selgrade MK, Bowman CC, Ladics GS, Privalle L, Laessig SA: Safety assessment of biotechnology products for potential risk of food allergy: implications of new research. Toxicol Sci 2009;110:31-39.
- Van Elburg RM, Heymanns HS, De Monchy JG: Effect of disodiumcromoglycate on intestinal permeability changes and clinical response during cow's milk challenge. Pediatr Allergy Immunol 1993;4:79-85.
- Jalonen T: Identical intestinal permeability changes in children with different clinical manifestations of cow's milk allergy. J Allergy Clin Immunol 1991;88:737-742.
- Heymann M, Desjeux JF: Cytokine-induced alteration of the epithelial barrier to food antigens in disease. Ann NY Acad Sci 2000;915:303-311.
- Hudson TJ: Skin barrier function and allergic risk. Nature Genetics 2006;38:399-400.
- Fu TJ, Abbott UR, Hatzos C: Digestibility of food allergens and nonallergenic proteins in simulated gastric fluid and simulated intestinal fluid - a comparative study. J Agric Food Chem 2002;50:7154-7160.
- Scurlock AM, Burks AW: Peanut allergenicity. Ann Allergy Asthma Immunol 2004;93(suppl 3):S12-S18.
- Worm M, Edenharter G, Ruëff F, Scherer K, Pföhler C, Mahler V, Treudler R, Lang R, Nemat K, Koehli A, Niggemann B, Hompes S: Symptom profile and risk factors of anaphylaxis in Central Europe. Allergy 2012;67:691-698.
- Hompes S, Koehli A, Nemat K, Scherer K, Lange L, Rueff F, Rietschel E, Reese T, Szepfalusi Z, Schwerk N, Beyer K, Hawranek T, Niggemann B, Worm M: Provoking allergens and treatment of anaphylaxis in children and adolescents - data from the anaphylaxis registry of German-speaking countries. Pediatr Allergy Immunol 2011;22:568-574.
- Dinakar C: Anaphylaxis in children: current understanding and key issues in diagnosis and treatment. Curr Allergy Asthma Rep 2012;12:641-649.
- Kroghsbo S, Bogh KL, Rigby NM, Mills ENC, Rogers A, Madsen CB: Sensitization with 7S globulins from peanut, hazelnut, soy or pea induces IgE with different biological activities which are modified by soy tolerance. Int Arch Allergy Immunol 2011;155:212-224.
- Wensing M, Knulst AC, Piersma S, O'Kane F, Knol EF, Koppelman SJ: Patients with anaphylaxis to pea can have peanut allergy caused by cross-reactive IgE to vicilin (Ara h 1). J Allergy Clin Immunol 2003;111:420-424.
- Wilson S, Blaschek K, de Mejia E: Allergenic proteins in soybean: processing and reduction of P34 allergenicity. Nutr Rev 2005;63:47-58.
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