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Original Paper

High Cut-Off Hemofiltration versus Standard Hemofiltration: A Pilot Assessment of Effects on Indices of Apoptosis

Atan R.a · Virzi G.M.b, c · Peck L.d · Ramadas A.a · Brocca Ab, c · Eastwood G.d · Sood S.a · Ronco C.b, c · Bellomo R.d, e · Goehl H.f · Storr M.f

Author affiliations

aClinical School Johor Bahru, Jeffrey Cheah School of Medicine and Health Sciences, Monash University Malaysia, Johor Bahru, Malaysia; bDepartment of Nephrology, Dialysis and Transplant, San Bortolo Hospital, San Bortolo, and cInternational Renal Research Institute Vicenza, IRRIV, Vicenza, Italy; dDepartment of Intensive Care, Austin Hospital, Heidelberg, Vic., and eAustralian and New Zealand Intensive Care Research Centre, Melbourne, Vic., Australia; fGambro Dialysatoren GmbH, Research and Development, Hechingen, Germany

Related Articles for ""

Blood Purif 2014;37:296-303

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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: February 25, 2014
Accepted: April 25, 2014
Published online: August 01, 2014
Issue release date: October 2014

Number of Print Pages: 8
Number of Figures: 5
Number of Tables: 1

ISSN: 0253-5068 (Print)
eISSN: 1421-9735 (Online)

For additional information: https://www.karger.com/BPU

Abstract

Objectives: To measure plasma pro-apoptotic and pro-necrotic activity in severe acute kidney injury (AKI) patients within a randomized controlled trial of continuous veno-venous hemofiltration with high cut-off filters (CVVH-HCO) versus standard filters (CVVH-Std). Methods: We measured pro-apoptotic and pro-necrotic plasma activity by trypan blue exclusion cell viability assay, detection of DNA fragmentation, and by determination of caspase-3 activity and annexin V-based apoptosis and necrosis detection assay. Results: Compared to no apoptosis or necrosis after incubation with healthy plasma, 14-18% of cells showed apoptosis and 4-8% showed necrosis after incubation with plasma from AKI patients. When comparing different measures of pro-apoptotic or pro-necrotic activity, CVVH-HCO and CVVH-Std showed no differential effects on such activity, which remained high over the first 3 days of treatment. However, using annexin V-FITC, there was a significant drop in pro-apoptotic activity across the filter for the CVVH-HCO group (p = 0.043) but not for the CVVH-Std group (p = 0.327) and a significant difference between the two groups (CVVH-HCO vs. CVVH-Std p = 0.006). Conclusions: Patients with severe AKI have increased pro-apoptotic and pro-necrotic activity. Although on single-pass effect assessment, CVVH-HCO was superior to CVVH-Std in decreasing annexin V-FITC-assessed pro-apoptotic activity, there was no overall attenuation of such activity during the first 3 days of treatment.

© 2014 S. Karger AG, Basel


References

  1. Lydon A, Martyn JA: Apoptosis in critical illness. Int Anesthesiol Clin 2003;41:65-77.
  2. Wan L, Bagshaw SM, Langenberg C, Saotome T, May C, Bellomo R: Pathophysiology of septic acute kidney injury: what do we really know? Crit Care Med 2008;36(suppl 4):S198-S203.
  3. Rosen S, Heyman SN: Difficulties in understanding human ‘acute tubular necrosis': limited data and flawed animal models. Kidney Int 2001;60:1220-1224.
  4. Kaushal GP, Basnakian AG, Shah SV: Apoptotic pathways in ischemic acute renal failure. Kidney Int 2004;66:500-506.
  5. Linkermann A, De Zen F, Weinberg J, Kunzendorf U, Krautwald S: Programmed necrosis in acute kidney injury. Nephrol Dial Transplant 2012;27:3412-3419.
  6. Hotchkiss RS, Swanson PE, Freeman BD, Tinsley KW, Cobb JP, Matuschak GM, Buchman TG, Karl IE: Apoptotic cell death in patients with sepsis, shock, and multiple organ dysfunction. Crit Care Med 1999;27:1230-1251.
  7. Jacobs R, Honore PM, Joannes-Boyau O, Boer W, De Regt J, De Waele E, et al: Septic acute kidney injury: the culprit is inflammatory apoptosis rather than ischemic necrosis. Blood Purif 2011;32:262-265.
  8. Bordoni V, Bolgan I, Brendolan A, Crepaldi C, Gastaldon F, D'intini V, et al: Caspase-3 and -8 activation and cytokine removal with a novel cellulose triacetate super-permeable membrane in an in vitro sepsis model. Int J Artif Organs 2003;26:897-905.
    External Resources
  9. Cantaluppi V, Assenzio B, Pasero D, Romanazzi GM, Pacitti A, Lanfranco G, et al: Polymyxin-B hemoperfusion inactivates circulating proapoptotic factors. Intensive Care Med 2008;34:1638-1645.
  10. Boschetti-de-Fierro A, Voigt M, Storr M, Krause B: Extended characterization of a new class of membranes for blood purification: the high cut-off membranes. Int J Artif Organs 2013;36:455-463.
  11. Morgera S, Rocktäschel J, Haase M, Lehmann C, von Heymann C, Ziemer S, Priem F, Hocher B, Göhl H, Kox WJ, Buder HW, Neumayer HH: Intermittent high permeability hemofiltration in septic patients with acute renal failure. Intensive Care Med 2003;29:1989-1995.
  12. Atan R, Crosbie D, Bellomo R: Techniques of extracorporeal cytokine removal: a systematic review of the literature. Blood Purif 2012;33:88-100.
  13. Atan R, Crosbie D, Bellomo R: Techniques of extracorporeal cytokine removal: a systematic review of the literature on animal experimental studies. Int J Artif Organs 2013;36:149-158.
  14. Atan R, Crosbie DC, Bellomo R: Techniques of extracorporeal cytokine removal: a systematic review of human studies. Ren Fail 2013;35:1061-1070.
  15. Sundström C, Nilsson K: Establishment and characterization of a human histiocytic lymphoma cell line (U937). Int J Cancer 1976;17:565-577.
  16. Martin SJ, Reutelingsperger CP, McGahon AJ, Rader JA, van Schie RC, LaFace DM, Green DR: Early redistribution of plasma membrane phosphatidylserine is a general feature of apoptosis regardless of the initiating stimulus: inhibition by overexpression of Bcl-2 and Abl. J Exp Med 1995;182:1545-1556.
  17. Fadok VA, Voelker DR, Campbell PA, Cohen JJ, Bratton DL, Henson PM: Exposure of phosphatidylserine on the surface of apoptotic lymphocytes triggers specific recognition and removal by macrophages. J Immunol 1992;148:2207-2216.
    External Resources
  18. Diaz C, Schroit AJ: Role of translocases in the generation of phosphatidylserine asymmetry. J Membr Biol 1996;151:1-9.
  19. Homburg CH, de Haas M, von dem Borne AE, Verhoeven AJ, Reutelingsperger CP, Roos D: Human neutrophils lose their surface Fc gamma RIII and acquire Annexin V binding sites during apoptosis in vitro. Blood 1995;85:532-540.
    External Resources
  20. Verhoven B, Schlegel RA, Williamson P: Mechanisms of phosphatidylserine exposure, a phagocyte recognition signal, on apoptotic T lymphocytes. J Exp Med 1995;182:1597-1601.
  21. Bilbault P, Lavaux T, Lahlou A, Uring-Lambert B, Gaub MP, Ratomponirina C, et al: Transient Bcl-2 gene down-expression in circulating mononuclear cells of severe sepsis patients who died despite appropriate intensive care. Intensive Care Med 2004;30:408-415.
  22. Giamarellos-Bourboulis EJ, Routsi C, Plachouras D, Markaki V, Raftogiannis M, Zervakis D, et al: Early apoptosis of blood monocytes in the septic host: is it a mechanism of protection in the event of septic shock? Crit Care 2006;10:R76.
  23. Weiss M, Elsharkawi M, Welt K, Schneider EM: Transient leukocytosis, granulocyte colony-stimulating factor plasma concentrations, and apoptosis determined by binding of Annexin V by peripheral leukocytes in patients with severe sepsis. Ann NY Acad Sci 2003;1010:742-747.
  24. Livigni S, Bertolini G, Rossi C, Ferrari F, Giardino M, Pozzato M, Remuzzi G: Efficacy of coupled plasma filtration adsorption in patients with septic shock: a multicenter randomised controlled clinical trial. BMJ Open 2014;4:e003536.
  25. Rachoin JS, Foster D, Dellinger RP: Endotoxin removal: how far from the evidence? From EUPHAS to EUPHRATES. Contrib Nephrol. Basel, Karger, 2010, vol 167, pp 111-118.
  26. Cruz DN, Antonelli M, Fumagalli R, Foltran F, Brienza N, Donati A, Malcangi V, Petrini F, Volta G, Bobbio Pallavicini FM, Rottoli F, Giunta F, Ronco C: Early use of polymyxin B hemoperfusion in abdominal septic shock: the EUPHAS randomized controlled trial. JAMA 2009;301:2445-2452.
  27. Havasi A, Borkan SC: Apoptosis and acute kidney injury. Kidney Int 2011;80:29-40.

Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: February 25, 2014
Accepted: April 25, 2014
Published online: August 01, 2014
Issue release date: October 2014

Number of Print Pages: 8
Number of Figures: 5
Number of Tables: 1

ISSN: 0253-5068 (Print)
eISSN: 1421-9735 (Online)

For additional information: https://www.karger.com/BPU


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