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Original Article

Karyotype Analysis and Chromosomal Distribution of Repetitive DNA Sequences of Cucumis metuliferus Using Fluorescence in situ Hybridization

Yagi K.a · Siedlecka E.a · Pawełkowicz M.a · Wojcieszek M.a · Przybecki Z.a · Tagashira N.b · Hoshi Y.c · Malepszy S.a · Pląder W.a

Author affiliations

aDepartment of Plant Genetics, Breeding and Biotechnology, Faculty of Horticulture, Biotechnology and Landscape Architecture, Warsaw University of Life Sciences - SGGW, Warsaw, Poland; bDepartment of Living Design and Information Science, Faculty of Human Development, Hiroshima Jogakuin University, Hiroshima, and cDepartment of Plant Science, Tokai University, Kumamoto, Japan

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Cytogenet Genome Res 2014;144:237-242

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Article / Publication Details

First-Page Preview
Abstract of Original Article

Accepted: September 15, 2014
Published online: November 15, 2014
Issue release date: February 2015

Number of Print Pages: 6
Number of Figures: 2
Number of Tables: 0

ISSN: 1424-8581 (Print)
eISSN: 1424-859X (Online)

For additional information: http://www.karger.com/CGR

Abstract

Cucumis metuliferus (2n = 24) is a cultivated species of the Cucumis genus which is a potential genetic resource for Cucumis crops. Although some cytogenetic research has been reported, there is no study of karyotyping in this species. Here, we used 4′,6-diamidino-2-phenylindole and chromomycin A3 staining to identify 12 pairs of chromosomes in early-metaphase cells. Fluorescence in situ hybridization revealed the chromosomal distribution patterns of the 5S and 45S ribosomal DNA (rDNA) genes, telomeres, and 3 different satellite repeats. The 2 major signals of the 45S rDNA were located on the satellite of chromosome 11, and the 2 signals of the 5S rDNA and 2 minor signals of the 45S rDNA were located on chromosome 12. The telomere probes hybridized to the ends of all chromosomes. The 3 satellite DNAs were localized at the ends of chromosomes 1, 2, 4-10, and at the end of the short arm of chromosome 3. In summary, we reported the identification of all chromosomes of C. metuliferus. We also depicted the location of 5S and 45S rDNA, the telomere motif sequence, CmetSat1, CmetSatT2, and CmetmSat1 in an ideogram.

© 2014 S. Karger AG, Basel


Article / Publication Details

First-Page Preview
Abstract of Original Article

Accepted: September 15, 2014
Published online: November 15, 2014
Issue release date: February 2015

Number of Print Pages: 6
Number of Figures: 2
Number of Tables: 0

ISSN: 1424-8581 (Print)
eISSN: 1424-859X (Online)

For additional information: http://www.karger.com/CGR


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