Cells Tissues Organs
Original Paper
Mechanical Force-Induced Specific MicroRNA Expression in Human Periodontal Ligament Stem CellsWei F.L.a, d · Wang J.H.b · Ding G.c · Yang S.Y.a · Li Y.a · Hu Y.J.a · Wang S.L.d, eaDepartment of Orthodontics, Shandong Provincial Key Laboratory of Oral Biomedicine, School of Stomatology, Shandong University, Jinan, bDepartment of Prosthodontics, Qindao Stomatological Hospital, Qingdao, cDepartment of Stomatology, Yidu Central Hospital, Weifang Medical University, Qingzhou, dSalivary Gland Disease Center and Molecular Laboratory for Gene Therapy and Tooth Regeneration, Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, Capital Medical University School of Stomatology, and eDepartment of Biochemistry and Molecular Biology, Capital Medical University School of Basic Medical Sciences, Beijing, PR China
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Cells Tissues Organs 2014;199:353-363
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Article / Publication Details
First-Page Preview
Accepted: November 06, 2014
Published online: March 24, 2015
Issue release date: April 2015
Number of Print Pages: 11
Number of Figures: 4
Number of Tables: 2
ISSN: 1422-6405 (Print)
eISSN: 1422-6421 (Online)
For additional information: https://www.karger.com/CTO
Abstract
It remains unclear how the expression of microRNAs (miRNAs) in human periodontal ligament stem cells (PDLSCs) might respond to mechanical stretch. To investigate specific miRNA expression in stretched PDLSCs, we used a Flexcell® FX-5000™ tension system to achieve external mechanical stimulation. Then, a custom-designed microarray assay was performed to investigate and describe the genome-wide differential expression of miRNAs in normal and stretched PDLSCs. Finally, we implemented integrative miRNA target prediction and network analysis approaches to construct an interaction network of the key miRNAs and their putative targets. We found that stretching induced morphological changes and increased alkaline phosphatase (ALP) activity, runt-related transcription factor 2 (RUNX2), osteocalcin (OCN), and bone sialoprotein (BSP) expression in PDLSCs. The microarray data showed that 53 miRNAs were differentially expressed with stretching. With an interaction network, we examined the connections between 10 selected key miRNAs and their putative target genes, which were related to mechanical force. The results from the interaction network provided a basis for postulating the functional roles of miRNAs in PDLSCs.
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Article / Publication Details
First-Page Preview
Accepted: November 06, 2014
Published online: March 24, 2015
Issue release date: April 2015
Number of Print Pages: 11
Number of Figures: 4
Number of Tables: 2
ISSN: 1422-6405 (Print)
eISSN: 1422-6421 (Online)
For additional information: https://www.karger.com/CTO
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