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Original Research

Nitric Oxide Synthase Inhibition Abolishes Exercise-Mediated Protection against Isoproterenol-Induced Cardiac Hypertrophy in Female Mice

Ren J.a · Yang L.b · Tian W.b · Zhu M.c · Liu J.b · Lu P.a · Li J.b · Yang L.b · Qi Z.c

Author affiliations

aDepartment of Parasitology, Basic Medical College, Tianjin Medical University, and Departments of bPharmacology and cHistology and Embryology, School of Medicine, Nankai University, Tianjin, China

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Cardiology 2015;130:175-184

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Article / Publication Details

First-Page Preview
Abstract of Original Research

Received: June 17, 2014
Accepted: November 20, 2014
Published online: February 25, 2015
Issue release date: March 2015

Number of Print Pages: 10
Number of Figures: 6
Number of Tables: 0

ISSN: 0008-6312 (Print)
eISSN: 1421-9751 (Online)

For additional information: https://www.karger.com/CRD

Abstract

Objective: Exercise training (ET) provides a cardioprotective effect against pathological cardiac hypertrophy. Nitric oxide (NO) plays an important role in modulating cardiac hypertrophy. However, few studies explore the relationship between NO signaling and the inhibitory effect of ET on pathological cardiac remodeling. Methods: In this study, we evaluated ET effects on isoproterenol (ISO)-induced cardiac hypertrophy in female mice. Moreover, L-NAME (Nω-nitro-L-arginine methyl ester), a nonselective NO synthase (NOS) inhibitor, was used to assess the involvement of NO signaling in cardiac hypertrophy. Morphological and echocardiographic variables were assessed. Cardiac hypertrophy-related gene expression was detected by real-time PCR and the protein levels of NOS signaling molecules were determined by Western blot. Results:L-NAME treatment prevented the beneficial effects of ET against the increase in heart weight (HW)/body weight (BW), HW/tibia length and lung weight/BW and echocardiographic variables following ISO injection. Also, L-NAME co-administration reversed ET-induced inhibition of myocardial fibrosis and fetal gene reactivation in ISO-treated mice. Furthermore, L-NAME treatment prevented ET-mediated up-regulation of phosphorylated endothelial NOS and plasma NO in ISO-treated mice. Conclusions: Our findings demonstrate that L-NAME treatment could abolish ET-induced cardioprotection against pathological cardiac hypertrophy and that NOS modulation may be involved in the antihypertrophic effects induced by ET.

© 2015 S. Karger AG, Basel


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Article / Publication Details

First-Page Preview
Abstract of Original Research

Received: June 17, 2014
Accepted: November 20, 2014
Published online: February 25, 2015
Issue release date: March 2015

Number of Print Pages: 10
Number of Figures: 6
Number of Tables: 0

ISSN: 0008-6312 (Print)
eISSN: 1421-9751 (Online)

For additional information: https://www.karger.com/CRD


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