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Translational Thyroidology / Original Paper

Free Access

Differences in Mouse Hepatic Thyroid Hormone Transporter Expression with Age and Hyperthyroidism

Engels K.a · Rakov H.a · Zwanziger D.a · Moeller L.C.a · Homuth G.b · Köhrle J.c · Brix K.d · Führer D.a

Author affiliations

aDepartment of Endocrinology and Metabolism, University Hospital Essen, Essen, bDepartment of Functional Genomics, Ernst-Moritz-Arndt University Greifswald, Greifswald, cInstitute of Experimental Endocrinology, Charité-Universitätsmedizin Berlin, Berlin, and dDepartment of Life Sciences and Chemistry, Jacobs University Bremen, Bremen, Germany

Corresponding Author

Dagmar Führer, MD, PhD

Department of Endocrinology and Metabolism and Division of Laboratory Research

University Hospital Essen, Hufelandstrasse 55

DE-45147 Essen (Germany)

E-Mail dagmar.fuehrer@uk-essen.de

Related Articles for ""

Eur Thyroid J 2015;4(suppl 1):81-86

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Abstract

Background: Clinical features of thyroid dysfunction vary with age, and an oligosymptomatic presentation of hyperthyroidism is frequently observed in the elderly. This suggests age modulation of thyroid hormone (TH) action, which may occur, for example, by alterations in TH production, metabolism and/or TH action in target organs. Objectives: In this paper, we address possible changes in TH transporter expression in liver tissues as a mechanism of age-dependent variation in TH action. Methods: Chronic hyperthyroidism was induced in 4- and 20-month-old C57BL6/NTac male mice (n = 8-10) by intraperitoneal injections of 1 µg/g body weight L-thyroxine (T4) every 48 h over 7 weeks. Control animals were injected with PBS. Total RNA was isolated from liver samples for analysis of the TH transporter and TH-responsive gene expression. TH concentrations were determined in mice sera. Results: Baseline serum free T4 (fT4) concentrations were significantly higher in euthyroid young compared to old mice. T4 treatment increased total T4, fT4 and free triiodothyronine to comparable concentrations in young and old mice. In the euthyroid state, TH transporter expression was significantly higher in old than in young mice, except for Mct8 and Oatp1a1 expression levels. Hyperthyroidism resulted in upregulation of Mct10, Lat1 and Lat2 in liver tissue, while Oatp1a1, Oatp1b2 and Oatp1a4 expression was downregulated. This effect was preserved in old animals. Conclusion: Here, we show age-dependent differences in TH transporter mRNA expression in the euthyroid and hyperthyroid state of mice focusing on the liver as a classical TH target organ.

© 2015 European Thyroid Association Published by S. Karger AG, Basel


Article / Publication Details

First-Page Preview
Abstract of Translational Thyroidology / Original Paper

Received: November 18, 2014
Accepted: February 16, 2015
Published online: May 27, 2015
Issue release date: September 2015

Number of Print Pages: 6
Number of Figures: 4
Number of Tables: 0

ISSN: 2235-0640 (Print)
eISSN: 2235-0802 (Online)

For additional information: http://www.karger.com/ETJ


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