Development and Characterization of an Engraftable Tissue-Cultured Skin Autograft: Alternative Treatment for Severe Electrical Injuries?Golinski P.a · Menke H.c · Hofmann Ma · Valesky E.a · Butting M.a · Kippenberger S.a · Bereiter-Hahn J.b · Bernd A.a · Kaufmann R.a · Zoeller N.N.a
aDepartment of Dermatology, Venereology and Allergology, University Hospital, and bKinematic Cell Research Group, Goethe University, Frankfurt am Main, and cClinic of Plastic, Aesthetic and Hand Surgery Burn Center, Offenbach am Main, Germany
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Background/Aims: Optimizing the treatment regimens of extensive or nonhealing defects is a constant challenge. Tissue-cultured skin autografts may be an alternative to mesh grafts and keratinocyte suspensions that are applied during surgical defect coverage. Methods: Autologous epidermal and dermal cells were isolated, in vitro expanded and seeded on collagen-elastin scaffolds. The developed autograft was immunohistochemically and electron microscopically characterized. Subsequently, it was transplanted onto lesions of a severely burned patient. Results: Comparability of the skin equivalent to healthy human skin could be shown due to the epidermal strata, differentiation, proliferation markers and development of characteristics of a functional basal lamina. Approximately 2 weeks after skin equivalent transplantation the emerging new skin correlated closely to the adjacent normal skin. Conclusion: The present study demonstrates the comparability of the developed organotypic skin equivalent to healthy human skin and its versatility for clinical applications.
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