Login to MyKarger

New to MyKarger? Click here to sign up.



Login with Facebook

Forgot your password?

Authors, Editors, Reviewers

For Manuscript Submission, Check or Review Login please go to Submission Websites List.

Submission Websites List

Institutional Login
(Shibboleth or Open Athens)

For the academic login, please select your country in the dropdown list. You will be redirected to verify your credentials.

Original Paper

Development and Characterization of an Engraftable Tissue-Cultured Skin Autograft: Alternative Treatment for Severe Electrical Injuries?

Golinski P.a · Menke H.c · Hofmann Ma · Valesky E.a · Butting M.a · Kippenberger S.a · Bereiter-Hahn J.b · Bernd A.a · Kaufmann R.a · Zoeller N.N.a

Author affiliations

aDepartment of Dermatology, Venereology and Allergology, University Hospital, and bKinematic Cell Research Group, Goethe University, Frankfurt am Main, and cClinic of Plastic, Aesthetic and Hand Surgery Burn Center, Offenbach am Main, Germany

Related Articles for ""

Cells Tissues Organs 2014;200:227-239

Do you have an account?

Login Information





Contact Information












By signing up for MyKarger you will automatically participate in our year-End raffle.
If you Then Do Not wish To participate, please uncheck the following box.

Yes, I wish To participate In the year-End raffle And Get the chance To win some Of our most interesting books, And other attractive prizes.


I have read the Karger Terms and Conditions and agree.



Login Information





Contact Information












By signing up for MyKarger you will automatically participate in our year-End raffle.
If you Then Do Not wish To participate, please uncheck the following box.

Yes, I wish To participate In the year-End raffle And Get the chance To win some Of our most interesting books, And other attractive prizes.


I have read the Karger Terms and Conditions and agree.



To view the fulltext, please log in

To view the pdf, please log in

Buy

  • FullText & PDF
  • Unlimited re-access via MyKarger
  • Unrestricted printing, no saving restrictions for personal use
read more

CHF 38.00 *
EUR 35.00 *
USD 39.00 *

Select

KAB

Buy a Karger Article Bundle (KAB) and profit from a discount!

If you would like to redeem your KAB credit, please log in.


Save over 20% compared to the individual article price.
Learn more

Rent/Cloud

  • Rent for 48h to view
  • Buy Cloud Access for unlimited viewing via different devices
  • Synchronizing in the ReadCube Cloud
  • Printing and saving restrictions apply

Rental: USD 8.50
Cloud: USD 20.00


Select

Subscribe

  • Access to all articles of the subscribed year(s) guaranteed for 5 years
  • Unlimited re-access via Subscriber Login or MyKarger
  • Unrestricted printing, no saving restrictions for personal use
read more

Subcription rates


Select

* The final prices may differ from the prices shown due to specifics of VAT rules.

Article / Publication Details

First-Page Preview
Abstract of Original Paper

Accepted: May 26, 2015
Published online: August 21, 2015
Issue release date: September 2015

Number of Print Pages: 13
Number of Figures: 8
Number of Tables: 0

ISSN: 1422-6405 (Print)
eISSN: 1422-6421 (Online)

For additional information: https://www.karger.com/CTO

Abstract

Background/Aims: Optimizing the treatment regimens of extensive or nonhealing defects is a constant challenge. Tissue-cultured skin autografts may be an alternative to mesh grafts and keratinocyte suspensions that are applied during surgical defect coverage. Methods: Autologous epidermal and dermal cells were isolated, in vitro expanded and seeded on collagen-elastin scaffolds. The developed autograft was immunohistochemically and electron microscopically characterized. Subsequently, it was transplanted onto lesions of a severely burned patient. Results: Comparability of the skin equivalent to healthy human skin could be shown due to the epidermal strata, differentiation, proliferation markers and development of characteristics of a functional basal lamina. Approximately 2 weeks after skin equivalent transplantation the emerging new skin correlated closely to the adjacent normal skin. Conclusion: The present study demonstrates the comparability of the developed organotypic skin equivalent to healthy human skin and its versatility for clinical applications.

© 2015 S. Karger AG, Basel


References

  1. Boyce, S.T., J.F. Hansbrough (1988) Biologic attachment, growth, and differentiation of cultured human epidermal keratinocytes on a graftable collagen and chondroitin-6-sulfate substrate. Surgery 103: 421-431.
    External Resources
  2. Cario-Andre, M., S. Briganti, M. Picardo, O. Nikaido, Y. Gall, J. Ginestar, A. Taieb (2002) Epidermal reconstructs: a new tool to study topical and systemic photoprotective molecules. J Photochem Photobiol B 68: 79-87.
  3. Cedidi, C.C., L. Wilkens, A. Berger, G. Ingianni (2007) Influence of human fibroblasts on development and quality of multilayered composite grafts in athymic nude mice. Eur J Med Res 12: 541-555.
    External Resources
  4. Centanni, J.M., J.A. Straseski, A. Wicks, J.A. Hank, C.A. Rasmussen, M.A. Lokuta, M.J. Schurr, K.N. Foster, L.D. Faucher, D.M. Caruso, A.R. Comer, B.L. Allen-Hoffmann (2011) StrataGraft skin substitute is well-tolerated and is not acutely immunogenic in patients with traumatic wounds: results from a prospective, randomized, controlled dose escalation trial. Ann Surg 253: 672-683.
  5. Cuono, C., R. Langdon, J. McGuire (1986) Use of cultured epidermal autografts and dermal allografts as skin replacement after burn injury. Lancet 1: 1123-1124.
  6. Curren, R.D., G.C. Mun, D.P. Gibson, M.J. Aardema (2006) Development of a method for assessing micronucleus induction in a 3D human skin model (EpiDerm). Mutat Res 607: 192-204.
  7. El-Ghalbzouri, A., E.N. Lamme, C. van Blitterswijk, J. Koopman, M. Ponec (2004) The use of PEGT/PBT as a dermal scaffold for skin tissue engineering. Biomaterials 25: 2987-2996.
  8. El-Ghalbzouri, A., M.F. Jonkman, R. Dijkman, M. Ponec (2005) Basement membrane reconstruction in human skin equivalents is regulated by fibroblasts and/or exogenously activated keratinocytes. J Invest Dermatol 124: 79-86.
  9. Gangemi, E.N., D. Gregori, P. Berchialla, E. Zingarelli, M. Cairo, D. Bollero, J. Ganem, R. Capocelli, F. Cuccuru, P. Cassano, D. Risso, M. Stella (2008) Epidemiology and risk factors for pathologic scarring after burn wounds. Arch Facial Plast Surg 10: 93-102.
  10. Gibbs, S., H.M. van den Hoogenband, G. Kirtschig, C.D. Richters, S.W. Spiekstra, M. Breetveld, R.J. Scheper, E.M. de Boer (2006) Autologous full-thickness skin substitute for healing chronic wounds. Br J Dermatol 155: 267-274.
  11. Gillitzer, R., M. Goebeler (2001) Chemokines in cutaneous wound healing. J Leukoc Biol 69: 513-521.
    External Resources
  12. Harrison, C.A., M.J. Heaton, C.M. Layton, N.S. Mac (2006) Use of an in vitro model of tissue-engineered human skin to study keratinocyte attachment and migration in the process of reepithelialization. Wound Repair Regen 14: 203-209.
  13. Hartmann-Fritsch, F., T. Biedermann, E. Braziulis, M. Meuli, E. Reichmann (2013) A new model for preclinical testing of dermal substitutes for human skin reconstruction. Pediatr Surg Int 29: 479-488.
  14. Hofmann, M., M. Guschel, A. Bernd, J. Bereiter-Hahn, R. Kaufmann, C. Tandi, H. Wiig, S. Kippenberger (2006) Lowering of tumor interstitial fluid pressure reduces tumor cell proliferation in a xenograft tumor model. Neoplasia 8: 89-95.
  15. Hollander, D., M. Stein, A. Bernd, J. Windolf, A. Pannike (1999) Autologous keratinocytes cultured on benzylester hyaluronic acid membranes in the treatment of chronic full-thickness ulcers. J Wound Care 8: 351-355.
  16. Horch, R.E., J. Kopp, U. Kneser, J. Beier, A.D. Bach (2005) Tissue engineering of cultured skin substitutes. J Cell Mol Med 9: 592-608.
  17. Jewell, L., R. Guerrero, A.R. Quesada, L.S. Chan, W.L. Garner (2007) Rate of healing in skin-grafted burn wounds. Plast Reconstr Surg 120: 451-456.
  18. Jones, J.E., E.A. Nelson, A. Al-Hity (2013) Skin grafting for venous leg ulcers. Cochrane Database Syst Rev 1: CD001737.
  19. Kamolz, L.P., M. Luegmair, N. Wick, B. Eisenbock, S. Burjak, R. Koller, G. Meissl, M. Frey (2005) The Viennese culture method: cultured human epithelium obtained on a dermal matrix based on fibroblast containing fibrin glue gels. Burns 31: 25-29.
  20. Kaufmann, R., D. Greiner, S. Kippenberger, A. Bernd (1998) Grafting of in vitro cultured melanocytes onto laser-ablated lesions in vitiligo. Acta Derm Venereol 78: 136-138.
  21. Kielty, C.M., M.J. Sherratt, C.A. Shuttleworth (2002) Elastic fibres. J Cell Sci 115: 2817-2828.
    External Resources
  22. Kondo, S., F. Kooshesh, D.N. Sauder (1997) Penetration of keratinocyte-derived cytokines into basement membrane. J Cell Physiol 171: 190-195.
  23. Kreis, R.W., D.P. Mackie, R.R. Hermans, A.R. Vloemans (1994) Expansion techniques for skin grafts: comparison between mesh and Meek island (sandwich-) grafts. Burns 20(suppl 1): S39-S42.
  24. Kumar, R.J., R.M. Kimble, R. Boots, S.P. Pegg (2004) Treatment of partial-thickness burns: a prospective, randomized trial using Transcyte. ANZ J Surg 74: 622-626.
  25. Kuroyanagi, Y., K. Kubo, H. Matsui, H.J. Kim, S. Numari, Y. Mabuchi, S. Kagawa (2004) Establishment of banking system for allogeneic cultured dermal substitute. Artif Organs 28: 13-21.
  26. Kurzen, H., C. Henrich, D. Booken, N. Poenitz, A. Gratchev, C.D. Klemke, M. Engstner, S. Goerdt, N. Maas-Szabowski (2006) Functional characterization of the epidermal cholinergic system in vitro. J Invest Dermatol 126: 2458-2472.
  27. Lee, J.H., H.T. An, J.H. Chung, K.H. Kim, H.C. Eun, K.H. Cho (2002) Acute effects of UVB radiation on the proliferation and differentiation of keratinocytes. Photodermatol Photoimmunol Photomed 18: 253-261.
  28. Li, H., Y. Chu, Z. Zhang, G. Zhang, L. Jiang, H. Wu, S. Liu, C. Yu, Y. Jin (2012) Construction of bilayered tissue-engineered skin with human amniotic mesenchymal cells and human amniotic epithelial cells. Artif Organs 36: 911-919.
  29. Madry, R., J. Struzyna, A. Stachura-Kulach, L. Drozdz, M. Bugaj (2011) Effectiveness of Suprathel® application in partial thickness burns, frostbites and Lyell syndrome treatment. Pol Przegl Chir 83: 541-548.
  30. Marcelo, D., P.M. Beatriz, R. Jussara, B. Fabiana (2012) Tissue therapy with autologous dermal and epidermal culture cells for diabetic foot ulcers. Cell Tissue Bank 13: 241-249.
  31. Meissner, M., M. Doll, I. Hrgovic, G. Reichenbach, V. Konig, T. Hailemariam-Jahn, J. Gille, R. Kaufmann (2011a) Suppression of VEGFR2 expression in human endothelial cells by dimethylfumarate treatment: evidence for anti-angiogenic action. J Invest Dermatol 131: 1356-1364.
  32. Meissner, M., I. Hrgovic, M. Doll, R. Kaufmann (2011b) PPARδ agonists suppress angiogenesis in a VEGFR2-dependent manner. Arch Dermatol Res 303: 41-47.
  33. Menon, S., Z. Li, J.G. Harvey, A.J. Holland (2013) The use of the Meek technique in conjunction with cultured epithelial autograft in the management of major paediatric burns. Burns 39: 674-679.
  34. Mewes, K.R., M. Raus, A. Bernd, N.N. Zoller, A. Sattler, R. Graf (2007) Elastin expression in a newly developed full-thickness skin equivalent. Skin Pharmacol Physiol 20: 85-95.
  35. Mohammadi, A.A., A.R. Bakhshaeekia, S. Marzban, S. Abbasi, A.R. Ashraf, M.K. Mohammadi, H.R. Toulide-Ie, A.R. Tavakkolian (2011) Early excision and skin grafting versus delayed skin grafting in deep hand burns (a randomised clinical controlled trial). Burns 37: 36-41.
  36. Oh, J.W., T.C. Hsi, C.F. Guerrero-Juarez, R. Ramos, M.V. Plikus (2013) Organotypic skin culture. J Invest Dermatol 133: e14.
  37. Onuma, H., C. Mastui, M. Morohashi (2001) Quantitative analysis of the proliferation of epidermal cells using a human skin organ culture system and the effect of DbcAMP using markers of proliferation (BrdU, Ki-67, PCNA). Arch Dermatol Res 293: 133-138.
  38. Pauchot, J., A. Elkhyat, G. Rolin, S. Mac, A. Grumblat, A. Fotso, P. Humbert, Y. Tropet (2013) Dermal equivalents in oncology: benefit of one-stage procedure. Dermatol Surg 39: 43-50.
  39. Phillips, L.G., M.C. Robson, D.J. Smith, W.A. Phillips, W.D. Gracia, T.P. McHugh, W.G. Sullivan, K. Mathoney, K. Swartz, T. Meltzer (1989) Uses and abuses of a biosynthetic dressing for partial skin thickness burns. Burns 15: 254-256.
  40. Pinney, E., K. Liu, B. Sheeman, J. Mansbridge (2000) Human three-dimensional fibroblast cultures express angiogenic activity. J Cell Physiol 183: 74-82.
  41. Poskitt, K.R., A.H. James, E.R. Lloyd-Davies, J. Walton, C. McCollum (1987) Pinch skin grafting or porcine dermis in venous ulcers: a randomised clinical trial. Br Med J (Clin Res Ed) 294: 674-676.
  42. Rheinwald, J.G., H. Green (1975) Serial cultivation of strains of human epidermal keratinocytes: the formation of keratinizing colonies from single cells. Cell 6: 331-343.
  43. Roguet, R., C. Cohen, J. Leclaire, E. Tessonneaud, C. Gagne, M.H. Teissier (2000) Use of a standardized reconstructed epidermis kit to assess in vitro the tolerance and the efficacy of cosmetics. Int J Cosmet Sci 22: 409-419.
  44. Ryssel, H., E. Gazyakan, G. Germann, M. Ohlbauer (2008) The use of MatriDerm in early excision and simultaneous autologous skin grafting in burns - a pilot study. Burns 34: 93-97.
  45. Schlotmann, K., M. Kaeten, A.F. Black, O. Damour, M. Waldmann-Laue, T. Forster (2001) Cosmetic efficacy claims in vitro using a three-dimensional human skin model. Int J Cosmet Sci 23: 309-318.
  46. Schoepe, S., H. Schaecke, A. Bernd, N. Zoeller, K. Asadullah (2010) Identification of novel in vitro test systems for the determination of glucocorticoid receptor ligand-induced skin atrophy. Skin Pharmacol Physiol 23: 139-151.
  47. Schurr, M.J., K.N. Foster, J.M. Centanni, A.R. Comer, A. Wicks, A.L. Gibson, C.L. Thomas-Virnig, S.J. Schlosser, L.D. Faucher, M.A. Lokuta, B.L. Allen-Hoffmann (2009) Phase I/II clinical evaluation of StrataGraft: a consistent, pathogen-free human skin substitute. J Trauma 66: 866-873.
  48. Sibbald, R.G., R. Zuker, P. Coutts, S. Coelho, D. Williamson, D. Queen (2005) Using a dermal skin substitute in the treatment of chronic wounds secondary to recessive dystrophic epidermolysis bullosa: a case series. Ostomy Wound Manage 51: 22-46.
    External Resources
  49. Sterne, G.D., F. Fatah (2002) Grafts and Flaps. Surgery (Oxford) 20: 134-138.
    External Resources
  50. Ucuzian, A.A., A.A. Gassman, A.T. East, H.P. Greisler (2010) Molecular mediators of angiogenesis. J Burn Care Res 31: 158-175.
  51. Uhlig, C., M. Rapp, K.K. Dittel (2007) New strategies for the treatment of thermally injured hands with regard to the epithelial substitute Suprathel (in German). Handchir Mikrochir Plast Chir 39: 314-319.
  52. van den Broek, L.J., F.B. Niessen, R.J. Scheper, S. Gibbs (2012) Development, validation and testing of a human tissue engineered hypertrophic scar model. ALTEX 29: 389-402.
  53. Wojtowicz, A.M., S. Oliveira, M.W. Carlson, A. Zawadzka, C.F. Rousseau, D. Baksh (2014) The importance of both fibroblasts and keratinocytes in a bilayered living cellular construct used in wound healing. Wound Repair Regen 22: 246-255.
  54. Zamora, D.O., S. Natesan, S. Becerra, N. Wrice, E. Chung, L.J. Suggs, R.J. Christy (2013) Enhanced wound vascularization using a dsASCs seeded FPEG scaffold. Angiogenesis 16: 745-757.
  55. Zöller, N.N., S. Kippenberger, D. Thaci, K. Mewes, M. Spiegel, A. Sattler, M. Schultz, J. Bereiter-Hahn, R. Kaufmann, A. Bernd (2008) Evaluation of beneficial and adverse effects of glucocorticoids on a newly developed full-thickness skin model. Toxicol In Vitro 22: 747-759.
  56. Zöller, N., E. Valesky, M. Butting, M. Hofmann, S. Kippenberger, J. Bereiter-Hahn, A. Bernd, R. Kaufmann (2014) Clinical application of a tissue-cultured skin autograft: an alternative for the treatment of non-healing or slowly healing wounds? Dermatology 229: 190-198.

Article / Publication Details

First-Page Preview
Abstract of Original Paper

Accepted: May 26, 2015
Published online: August 21, 2015
Issue release date: September 2015

Number of Print Pages: 13
Number of Figures: 8
Number of Tables: 0

ISSN: 1422-6405 (Print)
eISSN: 1422-6421 (Online)

For additional information: https://www.karger.com/CTO


Copyright / Drug Dosage / Disclaimer

Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher.
Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.