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Research Paper

Imaging Mass Spectrometry Reveals a Unique Distribution of Triglycerides in the Abdominal Aortic Aneurysmal Wall

Tanaka H.a-c · Zaima N.b, e · Sasaki T.d · Yamamoto N.a, c · Inuzuka K.a, c · Sano M.a, c · Saito T.a, c · Hayasaka T.b, f · Goto-Inoue N.b, g · Sato K.d · Kugo H.e · Moriyama T.e · Konno H.c · Setou M.b, h · Unno N.a, c

Author affiliations

aDivision of Vascular Surgery, bDepartment of Cell Biology and Anatomy, cSecond Department of Surgery and dDepartment of Anatomy and Neuroscience, Hamamatsu University School of Medicine, Hamamatsu, eDepartment of Applied Biological Chemistry, Graduate School of Agriculture, Kinki University, Higashiosaka, fHealth Innovation and Technology Center, Hokkaido University, Sapporo, and gDepartment of Health Promotion Science, Tokyo Metropolitan University, Tokyo, Japan; hDepartment of Anatomy, Hong Kong University, Hong Kong, SAR, PR China

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J Vasc Res 2015;52:127-135

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Article / Publication Details

First-Page Preview
Abstract of Research Paper

Received: January 23, 2015
Accepted: July 30, 2015
Published online: September 09, 2015
Issue release date: September 2015

Number of Print Pages: 9
Number of Figures: 5
Number of Tables: 1

ISSN: 1018-1172 (Print)
eISSN: 1423-0135 (Online)

For additional information: https://www.karger.com/JVR

Abstract

The pathophysiology underlying abdominal aortic aneurysms (AAAs) remains unknown. In this study, we applied imaging mass spectrometry (IMS) to analyze the pathophysiology of the aneurysmal wall. Comparisons were performed between the tissue samples from the neck and the sac of the AAA, at a single time point, in 30 patients who underwent elective surgery of their AAAs. The localization of each lipid molecule in the aortic wall was assessed by IMS. Histopathological examination and IMS revealed a characteristic distribution of triglycerides (TGs) specifically in the aneurismal adventitia of the sac. This characteristic TG distribution was derived from an ectopic appearance of adipocytes in the adventitia. Furthermore, ectopic adipocyte accumulation in the aortic wall leads to the loss of the collagen fiber network subsequent to the wall rupture. The underlying mechanism of adipocyte accumulation involves the presence of adipose-derived stem cells (ADSCs) in the aneurismal adventitia and the expression of peroxisome proliferator-activated receptor gamma 2, a master regulator of adipocyte differentiation by some ADSCs. This study reveals new, previously overlooked aspects of AAA pathology.

© 2015 S. Karger AG, Basel


References

  1. Ashton HA, Buxton MJ, Day NE, Kim LG, Marteau TM, Scott RA, Thompson SG, Walker NM, Multicentre Aneurysm Screening Study Group: The Multicentre Aneurysm Screening Study (MASS) into the effect of abdominal aortic aneurysm screening on mortality in men: a randomised controlled trial. Lancet 2002;360:1531-1539.
  2. Lederle FA, Wilson SE, Johnson GR, Reinke DB, Littooy FN, Acher CW, Ballard DJ, Messina LM, Gordon IL, Chute EP, Krupski WC, Busuttil SJ, Barone GW, Sparks S, Graham LM, Rapp JH, Makaroun MS, Moneta GL, Cambria RA, Makhoul RG, Eton D, Ansel HJ, Freischlag JA, Bandyk D: Immediate repair compared with surveillance of small abdominal aortic aneurysms. N Engl J Med 2002;346:1437-1444.
  3. Sakalihasan N, Limet R, Defawe OD: Abdominal aortic aneurysm. Lancet 2005;365:1577-1589.
  4. Katz DA, Littenberg B, Cronenwett JL: Management of small abdominal aortic aneurysms: early surgery vs watchful waiting. JAMA 1992;268:2678-2686.
  5. United Kingdom EVAR Trial Investigators, Greenhalgh RM, Brown LC, Powell JT, Thompson SG, Epstein D, Sculpher MJ: Endovascular versus open repair of abdominal aortic aneurysm. New Engl J Med 2010;362:1863-1871.
  6. Holzapfel GA, Sommer G, Gasser CT, Regitnig P: Determination of layer-specific mechanical properties of human coronary arteries with nonatherosclerotic intimal thickening and related constitutive modeling. Am J Physiol Heart Circ Physiol 2005;289:H2048-H2058.
  7. Schulze-Bauer CA, Regitnig P, Holzapfel GA: Mechanics of the human femoral adventitia including the high-pressure response. Am J Physiol Heart Circ Physiol 2002;282:H2427-H2440.
  8. Tsuruda T, Kato J, Hatakeyama K, Kojima K, Yano M, Yano Y, Nakamura K, Nakamura-Uchiyama F, Matsushima Y, Imamura T, Onitsuka T, Asada Y, Nawa Y, Eto T, Kitamura K: Adventitial mast cells contribute to pathogenesis in the progression of abdominal aortic aneurysm. Circ Res 2008;102:1368-1377.
  9. Cornett DS, Reyzer ML, Chaurand P, Caprioli RM: MALDI imaging mass spectrometry: molecular snapshots of biochemical systems. Nat Methods 2007;4:828-833.
  10. Harada T, Yuba-Kubo A, Sugiura Y, Zaima N, Hayasaka T, Goto-Inoue N, Wakui M, Suematsu M, Takeshita K, Ogawa K, Yoshida Y, Setou M: Visualization of volatile substances in different organelles with an atmospheric-pressure mass microscope. Anal Chem 2009;81:9153-9157.
  11. Zaima N, Goto-Inoue N, Moriyama T: Matrix-assisted laser desorption/ionization imaging mass spectrometry: new technology for vascular pathology. J Vasc Res 2014;51:144-148.
  12. Tanaka H, Zaima N, Sasaki T, Yamamoto N, Sano M, Konno H, Setou M, Unno N: Loss of lymphatic vessels and regional lipid accumulation is associated with great saphenous vein incompetence. J Vasc Surg 2012;55:1440-1448.
  13. Tanaka H, Zaima N, Yamamoto N, Sagara D, Suzuki M, Nishiyama M, Mano Y, Sano M, Hayasaka T, Goto-Inoue N, Sasaki T, Konno H, Unno N, Setou M: Imaging mass spectrometry reveals unique lipid distribution in primary varicose veins. Eur J Vasc Endovasc Surg 2010;40:657-663.
  14. Tanaka H, Zaima N, Yamamoto N, Suzuki M, Mano Y, Konno H, Unno N, Setou M: Distribution of phospholipid molecular species in autogenous access grafts for hemodialysis analyzed using imaging mass spectrometry. Anal Bioanal Chem 2011;400:1873-1880.
  15. Zaima N, Sasaki T, Tanaka H, Cheng XW, Onoue K, Hayasaka T, Goto-Inoue N, Enomoto H, Unno N, Kuzuya M, Setou M: Imaging mass spectrometry-based histopathologic examination of atherosclerotic lesions. Atherosclerosis 2011;217:427-432.
  16. Hayasaka T, Goto-Inoue N, Sugiura Y, Zaima N, Nakanish H, Ohishi K, Nakanish S, Naito T, Taguchi R, Setou M: Matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight (MALDI-QIT-TOF)-based imaging mass spectrometry reveals a layered distribution of phospholipid molecular species in the mouse retina. Rapid Commun Mass Spectrom 2008;22:3415-3426.
  17. Zaima N, Hayasaka T, Goto-Inoue N, Setou M: Imaging of metabolites by MALDI mass spectrometry. J Oleo Sci 2009;58:415-419.
  18. Zaima N, Matsuyama Y, Setou M: Principal component analysis of direct matrix-assisted laser desorption/ionization mass spectrometric data related to metabolites of fatty liver. J Oleo Sci 2009;58:267-273.
  19. Hayasaka T, Goto-Inoue N, Zaima N, Kimura Y, Setou M: Organ-specific distributions of lysophosphatidylcholine and triacylglycerol in mouse embryo. Lipids 2009;44:837-848.
  20. Lin CS, Xin ZC, Deng CH, Ning H, Lin G, Lue TF: Defining adipose tissue-derived stem cells in tissue and in culture. Histol Histopathol 2010;25:807-815.
  21. Tang W, Zeve D, Suh JM, Bosnakovski D, Kyba M, Hammer RE, Tallquist MD, Graff JM: White fat progenitor cells reside in the adipose vasculature. Science 2008;322:583-586.
  22. Traktuev DO, Merfeld-Clauss S, Li J, Kolonin M, Arap W, Pasqualini R, Johnstone BH, March KL: A population of multipotent CD34-positive adipose stromal cells share pericyte and mesenchymal surface markers, reside in a periendothelial location, and stabilize endothelial networks. Circ Res 2008;102:77-85.
  23. Norrgard O, Angquist KA, Johnson O: Familial aortic aneurysms: serum concentrations of triglyceride, cholesterol, HDL-cholesterol and (VLDL + LDL)-cholesterol. Br J Surg 1985;72:113-116.
  24. Watt HC, Law MR, Wald NJ, Craig WY, Ledue TB, Haddow JE: Serum triglyceride: a possible risk factor for ruptured abdominal aortic aneurysm. Int J Epidemiol 1998;27:949-952.
  25. Bouloumie A, Sengenes C, Portolan G, Galitzky J, Lafontan M: Adipocyte produces matrix metalloproteinases 2 and 9: involvement in adipose differentiation. Diabetes 2001;50:2080-2086.
  26. Trayhurn P, Wood IS: Adipokines: inflammation and the pleiotropic role of white adipose tissue. Br J Nutr 2004;92:347-355.
  27. Stenmark KR, Yeager ME, El Kasmi KC, Nozik-Grayck E, Gerasimovskaya EV, Li M, Riddle SR, Frid MG: The adventitia: essential regulator of vascular wall structure and function. Ann Rev Physiol 2013;75:23-47.
  28. Miranville A, Heeschen C, Sengenes C, Curat CA, Busse R, Bouloumie A: Improvement of postnatal neovascularization by human adipose tissue-derived stem cells. Circulation 2004;110:349-355.
  29. Nathan S, Das De S, Thambyah A, Fen C, Goh J, Lee EH: Cell-based therapy in the repair of osteochondral defects: a novel use for adipose tissue. Tissue Eng 2003;9:733-744.
  30. Gimble JM, Katz AJ, Bunnell BA: Adipose-derived stem cells for regenerative medicine. Circ Res 2007;100:1249-1260.
  31. Gimble JM, Nuttall ME: Adipose-derived stromal/stem cells (ASC) in regenerative medicine: pharmaceutical applications. Curr Pharm Des 2011;17:332-339.
  32. Yoshimura K, Suga H, Eto H: Adipose-derived stem/progenitor cells: roles in adipose tissue remodeling and potential use for soft tissue augmentation. Regen Med 2009;4:265-273.
  33. Kim H, Mizuno M, Furuhashi K, Katsuno T, Ozaki T, Yasuda K, Tsuboi N, Sato W, Suzuki Y, Matsuo S, Ito Y, Maruyama S: Rat adipose tissue-derived stem cells attenuate peritoneal injuries in rat zymosan-induced peritonitis accompanied by complement activation. Cytotherapy 2014;16:357-368.
  34. Cai A, Qiu R, Li L, Zheng D, Dong Y, Yu D, Huang Y, Rao S, Zhou Y, Mai W: Atorvastatin treatment of rats with ischemia-reperfusion injury improves adipose-derived mesenchymal stem cell migration and survival via the SDF-1α/CXCR-4 axis. PLoS One 2013;8:e79100.
  35. Tontonoz P, Hu E, Spiegelman BM: Stimulation of adipogenesis in fibroblasts by PPARγ2, a lipid-activated transcription factor. Cell 1994;79:1147-1156.
  36. Rosen ED, Sarraf P, Troy AE, Bradwin G, Moore K, Milstone DS, Spiegelman BM, Mortensen RM: PPARγ is required for the differentiation of adipose tissue in vivo and in vitro. Mol Cell 1999;4:611-617.
  37. Li Y, Zhang J, Schopfer FJ, Martynowski D, Garcia-Barrio MT, Kovach A, Suino-Powell K, Baker PR, Freeman BA, Chen YE, Xu HE: Molecular recognition of nitrated fatty acids by PPARγ. Nat Struct Mol Biol 2008;15:865-867.
  38. Forman BM, Tontonoz P, Chen J, Brun RP, Spiegelman BM, Evans RM: 15-Deoxy-Δ12,14-prostaglandin J2 is a ligand for the adipocyte determination factor PPARγ. Cell 1995;83:803-812.
  39. Suzuki Y, Kamikawa T, Kobayashi A, Yamazaki N: Effects of l-carnitine on tissue levels of free fatty acid, acyl CoA, and acylcarnitine in ischemic heart. Adv Myocardiol 1983;4:549-557.
  40. Tanaka H, Zaima N, Sasaki T, Hayasaka T, Goto-Inoue N, Onoue K, Ikegami K, Morita Y, Yamamoto N, Mano Y, Sano M, Saito T, Sato K, Konno H, Setou M, Unno N: Adventitial vasa vasorum arteriosclerosis in abdominal aortic aneurysm. PLoS One 2013;8:e57398.
  41. Sano M, Sasaki T, Hirakawa S, Sakabe J, Ogawa M, Baba S, Zaima N, Tanaka H, Inuzuka K, Yamamoto N, Setou M, Sato K, Konno H, Unno N: Lymphangiogenesis and angiogenesis in abdominal aortic aneurysm. PLoS One 2014;9:e89830.
  42. Zampell JC, Yan A, Elhadad S, Avraham T, Weitman E, Mehrara BJ: CD4+ cells regulate fibrosis and lymphangiogenesis in response to lymphatic fluid stasis. PLoS One 2012;7:e49940.

Article / Publication Details

First-Page Preview
Abstract of Research Paper

Received: January 23, 2015
Accepted: July 30, 2015
Published online: September 09, 2015
Issue release date: September 2015

Number of Print Pages: 9
Number of Figures: 5
Number of Tables: 1

ISSN: 1018-1172 (Print)
eISSN: 1423-0135 (Online)

For additional information: https://www.karger.com/JVR


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