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Original Article

Evidence for Sex Chromosome Turnover in Proteid Salamanders

Sessions S.K.a · Bizjak Mali L.b · Green D.M.c · Trifonov V.d, e · Ferguson-Smith M.d

Author affiliations

aDepartment of Biology, Hartwick College, Oneonta, N.Y., USA; bDepartment of Biology, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia; cRedpath Museum, McGill University, Montreal, Que., Canada; dDepartment of Veterinary Medicine, University of Cambridge, Cambridge, UK; eInstitute of Molecular and Cellular Biology SB RAS, Novosibirsk, Russia

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Cytogenet Genome Res 2016;148:305-313

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Article / Publication Details

First-Page Preview
Abstract of Original Article

Accepted: February 29, 2016
Published online: June 29, 2016
Issue release date: August 2016

Number of Print Pages: 9
Number of Figures: 6
Number of Tables: 0

ISSN: 1424-8581 (Print)
eISSN: 1424-859X (Online)

For additional information: https://www.karger.com/CGR

Abstract

A major goal of genomic and reproductive biology is to understand the evolution of sex determination and sex chromosomes. Species of the 2 genera of the Salamander family Proteidae - Necturus of eastern North America, and Proteus of Southern Europe - have similar-looking karyotypes with the same chromosome number (2n = 38), which differentiates them from all other salamanders. However, Necturus possesses strongly heteromorphic X and Y sex chromosomes that Proteus lacks. Since the heteromorphic sex chromosomes of Necturus were detectable only with C-banding, we hypothesized that we could use C-banding to find sex chromosomes in Proteus. We examined mitotic material from colchicine-treated intestinal epithelium, and meiotic material from testes in specimens of Proteus, representing 3 genetically distinct populations in Slovenia. We compared these results with those from Necturus. We performed FISH to visualize telomeric sequences in meiotic bivalents. Our results provide evidence that Proteus represents an example of sex chromosome turnover in which a Necturus-like Y-chromosome has become permanently translocated to another chromosome converting heteromorphic sex chromosomes to homomorphic sex chromosomes. These results may be key to understanding some unusual aspects of demographics and reproductive biology of Proteus, and are discussed in the context of models of the evolution of sex chromosomes in amphibians.

© 2016 S. Karger AG, Basel


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Article / Publication Details

First-Page Preview
Abstract of Original Article

Accepted: February 29, 2016
Published online: June 29, 2016
Issue release date: August 2016

Number of Print Pages: 9
Number of Figures: 6
Number of Tables: 0

ISSN: 1424-8581 (Print)
eISSN: 1424-859X (Online)

For additional information: https://www.karger.com/CGR


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