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Short Communication

Editor's Choice - Free Access

Increased CD69 Expression on Peripheral Eosinophils from Patients with Food Protein-Induced Enterocolitis Syndrome

Wada T. · Matsuda Y. · Toma T. · Koizumi E. · Okamoto H. · Yachie A.

Author affiliations

Department of Pediatrics, School of Medicine, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University, Kanazawa, Japan

Corresponding Author

Correspondence to: Dr. Taizo Wada

Department of Pediatrics, School of Medicine

Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University

13-1 Takaramachi, Kanazawa 920-8641 (Japan)

E-Mail taizo@staff.kanazawa-u.ac.jp

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Int Arch Allergy Immunol 2016;170:201-205

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Background: Food protein-induced enterocolitis syndrome (FPIES) is an uncommon, non-IgE-mediated food allergy. We recently described a significant increase in fecal eosinophil-derived neurotoxin (EDN) after ingestion of the causative food. However, little is known about the activation status of circulating eosinophils in patients with an acute FPIES reaction. Methods: Surface CD69 expression was assessed by flow cytometry on peripheral eosinophils from 5 patients with FPIES before and after ingestion of the causative food. Fecal EDN was measured by enzyme-linked immunosorbent assay. Results: No eosinophil activation was observed before ingestion; however, a significant increase in CD69 expression on eosinophils after an acute FIPES reaction was demonstrated in all of the patients. There was no significant change in absolute eosinophil counts in the peripheral blood. The levels of fecal EDN increased on the day after ingestion of the causative food in all patients. Conclusion: These results suggest that circulating eosinophils as well as eosinophils in the intestinal mucosal tissue are activated in acute FPIES reactions and might be associated with systemic immune events in FPIES.

© 2016 S. Karger AG, Basel


  1. Nowak-Wegrzyn A, Katz Y, Mehr SS, Koletzko S: Non-IgE-mediated gastrointestinal food allergy. J Allergy Clin Immunol 2015;135:1114-1124.
  2. Berin MC: Immunopathophysiology of food protein-induced enterocolitis syndrome. J Allergy Clin Immunol 2015;135:1108-1113.
  3. Guibas GV, Tsabouri S, Makris M, Priftis KN: Food protein-induced enterocolitis syndrome: pitfalls in the diagnosis. Pediatr Allergy Immunol 2014;25:622-629.
  4. Wada T, Toma T, Muraoka M, Matsuda Y, Yachie A: Elevation of fecal eosinophil-derived neurotoxin in infants with food protein-induced enterocolitis syndrome. Pediatr Allergy Immunol 2014;25:617-619.
  5. Sicherer SH, Eigenmann PA, Sampson HA: Clinical features of food protein-induced enterocolitis syndrome. J Pediatr 1998;133:214-219.
  6. Nowak-Wegrzyn A, Sampson HA, Wood RA, Sicherer SH: Food protein-induced enterocolitis syndrome caused by solid food proteins. Pediatrics 2003;111:829-835.
    External Resources
  7. Mehr S, Kakakios A, Frith K, Kemp AS: Food protein-induced enterocolitis syndrome: 16-year experience. Pediatrics 2009;123:e459-e464.
  8. Powell GK: Food protein-induced enterocolitis of infancy: differential diagnosis and management. Compr Ther 1986;12:28-37.
    External Resources
  9. Urisu A, Ebisawa M, Mukoyama T, Morikawa A, Kondo N: Japanese guideline for food allergy. Allergol Int 2011;60:221-236.
  10. Toma T, Mizuno K, Okamoto H, Kanegane C, Ohta K, Ikawa Y, Miura M, Kuroda M, Niida Y, Koizumi S, Yachie A: Expansion of activated eosinophils in infants with severe atopic dermatitis. Pediatr Int 2005;47:32-38.
  11. Van Sickle GJ, Powell GK, McDonald PJ, Goldblum RM: Milk- and soy protein-induced enterocolitis: evidence for lymphocyte sensitization to specific food proteins. Gastroenterology 1985;88:1915-1921.
  12. Hoffman KM, Ho DG, Sampson HA: Evaluation of the usefulness of lymphocyte proliferation assays in the diagnosis of allergy to cow's milk. J Allergy Clin Immunol 1997;99:360-366.
  13. Chung HL, Hwang JB, Park JJ, Kim SG: Expression of transforming growth factor beta1, transforming growth factor type I and II receptors, and TNF-alpha in the mucosa of the small intestine in infants with food protein-induced enterocolitis syndrome. J Allergy Clin Immunol 2002;109:150-154.
  14. Morita H, Nomura I, Orihara K, Yoshida K, Akasawa A, Tachimoto H, Ohtsuka Y, Namai Y, Futamura M, Shoda T, Matsuda A, Kamemura N, Kido H, Takahashi T, Ohya Y, Saito H, Matsumoto K: Antigen-specific T-cell responses in patients with non-IgE-mediated gastrointestinal food allergy are predominantly skewed to T(h)2. J Allergy Clin Immunol 2013;131:590-592.
  15. Powell GK: Milk- and soy-induced enterocolitis of infancy. Clinical features and standardization of challenge. J Pediatr 1978;93:553-560.
  16. Kalach N, Kapel N, Waligora-Dupriet AJ, Castelain MC, Cousin MO, Sauvage C, Ba F, Nicolis I, Campeotto F, Butel MJ, Dupont C: Intestinal permeability and fecal eosinophil-derived neurotoxin are the best diagnosis tools for digestive non-IgE-mediated cow's milk allergy in toddlers. Clin Chem Lab Med 2013;51:351-361.
  17. Caubet JC, Ford LS, Sickles L, Jarvinen KM, Sicherer SH, Sampson HA, Nowak-Wegrzyn A: Clinical features and resolution of food protein-induced enterocolitis syndrome: 10-year experience. J Allergy Clin Immunol 2014;134:382-389.
  18. Pignatti P, Perfetti L, Galdi E, Pozzi V, Bossi A, Biale C, Moscato G: Increased CD69 expression on peripheral blood eosinophils after specific inhalation challenge. Allergy 2002;57:411-416.
  19. Johansson MW: Activation states of blood eosinophils in asthma. Clin Exp Allergy 2014;44:482-498.

Article / Publication Details

First-Page Preview
Abstract of Short Communication

Received: April 14, 2016
Accepted: July 27, 2016
Published online: September 06, 2016
Issue release date: September 2016

Number of Print Pages: 5
Number of Figures: 2
Number of Tables: 1

ISSN: 1018-2438 (Print)
eISSN: 1423-0097 (Online)

For additional information: https://www.karger.com/IAA

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