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Original Article

Interstitial NORs, Fragile Sites, and Chromosome Evolution: A Not So Simple Relationship - The Example of Melolontha melolontha and Genus Protaetia (Coleoptera: Scarabaeidae)

Dutrillaux A.-M. · Carton B. · Cacheux L. · Dutrillaux B.

Author affiliations

Institut de Systématique, Evolution, Biodiversité, ISYEB-UMR 7205-CNRS, MNHN, UMPC, EPHE, Muséum National d'Histoire Naturelle, Sorbonne Universités, Paris, France

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Cytogenet Genome Res 2016;149:304-311

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Article / Publication Details

First-Page Preview
Abstract of Original Article

Accepted: June 14, 2016
Published online: October 07, 2016
Issue release date: December 2016

Number of Print Pages: 8
Number of Figures: 6
Number of Tables: 0

ISSN: 1424-8581 (Print)
eISSN: 1424-859X (Online)

For additional information: https://www.karger.com/CGR

Abstract

In the present study, the origin of recurrent rearrangements involving chromosome 6 in 3.2% of cells of Melolontha melolontha (Coleoptera, Scarabaeidae) was investigated. Various chromosome staining techniques, including C-banding, Giemsa and silver staining, as well as fluorescence in situ hybridization with a human 28S rDNA probe, were applied to M. melolontha chromosome spreads. In addition, related species of the genera Melolontha and Protaetia were studied. On chromosome 6 of M. melolontha, there is a fragile site-like structure which corresponds to an interstitial nucleolus organizer region (NOR). Despite this instability, the NOR remains unique and interstitial in this species, as well as in the other species studied. It is proposed that the intercalary position of the NOR both facilitates the detection of its fragile site-like instability and correlates with its relative stability during evolution. We explain this apparent paradox by strong counter-selection for imbalances of the chromosome fragment distal to the interstitial NORs, which would recurrently occur in the progeny of translocation carriers. Thus, the frequent telomeric position of the NORs in most animal and plant taxa would have no functional rationale but would be the consequence of selection against the meiotic transmission of chromosome imbalances.

© 2016 S. Karger AG, Basel


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Article / Publication Details

First-Page Preview
Abstract of Original Article

Accepted: June 14, 2016
Published online: October 07, 2016
Issue release date: December 2016

Number of Print Pages: 8
Number of Figures: 6
Number of Tables: 0

ISSN: 1424-8581 (Print)
eISSN: 1424-859X (Online)

For additional information: https://www.karger.com/CGR


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