Login to MyKarger



Facebook Login

Forgot your password?
Sign up to MyKarger

Authors, Editors, Reviewers

Manuscript Submission, Check or Review Login can be found directly on our journal pages.


Journal Overview

Institutional Login

Karger supports the identity and access management tools Shibboleth and OpenAthens for subscribing institutions.


Institutional Login

Original Article

Classical and Molecular Cytogenetics of the Panther Genet Genetta maculata (Mammalia, Carnivora, Viverridae)

Matoso Silva R.a · Adega F.b · Kjöllerström H.J.a · Labuschagne K.c · Kotze A.c, d · Fernandes C.a · Chaves R.b · do Mar Oom M.a

Author affiliations

acE3c - Centre for Ecology, Evolution and Environmental Changes, Faculdade de Ciências, Universidade de Lisboa, Lisbon, and bLaboratory of Cytogenomics and Animal Genomics (CAG), Department of Genetics and Biotechnology (DGB), University of Trás-os-Montes and Alto Douro (UTAD), Vila Real, Portugal; cNational Zoological Gardens of South Africa, Pretoria, and dGenetics Department, University of the Free State, Bloemfontein, South Africa

Keywords: Fluorescence in situ hybridizationGenettaLarge-spotted genetsRestriction-enzyme bandingTelomeric probeViverrid

Related Articles for ""
Cytogenet Genome Res 2016;149:274-281
https://doi.org/10.1159/000450627

Do you have an account?

Forgot your password?

Login Information





Contact Information











I have read the Karger Terms and Conditions and agree.


Login Information





Contact Information











I have read the Karger Terms and Conditions and agree.


To view the fulltext, please log in
Forgot your password?

To view the pdf, please log in
Forgot your password?

Buy

  • FullText & PDF
  • Unlimited re-access via MyKarger
  • Unrestricted printing, no saving restrictions for personal use
read more
CHF 38.00 *
EUR 35.00 *
USD 39.00 *

Select

KAB

Buy a Karger Article Bundle (KAB) and profit from a discount!


If you would like to redeem your KAB credit, please log in.

Save over 20% compared to the individual article price.

Learn more

Rent/Cloud

  • Rent for 48h to view
  • Buy Cloud Access for unlimited viewing via different devices
  • Synchronizing in the ReadCube Cloud
  • Printing and saving restrictions apply
Rental: USD 8.50
Cloud: USD 20.00

Select

Subscribe

  • Access to all articles of the subscribed year(s) guaranteed for 5 years
  • Unlimited re-access via Subscriber Login or MyKarger
  • Unrestricted printing, no saving restrictions for personal use
read more

Subcription rates

Select
* The final prices may differ from the prices shown due to specifics of VAT rules.

Article / Publication Details

First-Page Preview
Abstract of Original Article

Accepted: August 08, 2016
Published online: October 27, 2016
Issue release date: December 2016

Number of Print Pages: 8
Number of Figures: 3
Number of Tables: 0

ISSN: 1424-8581 (Print)
eISSN: 1424-859X (Online)

For additional information: https://www.karger.com/CGR

Abstract

Genets (Genetta) are a genus of African mammalian carnivorans with 14 currently recognized species, although taxonomic uncertainties remain, particularly regarding the number of species within the large-spotted genet complex. This study presents the first banded karyotype and molecular cytogenetic analysis of a genetically identified panther genet, Genetta maculata, the most common and widespread taxon of the large-spotted genet complex, with a wide distribution in sub-Saharan Africa. Sampled in Gauteng Province, South Africa, it could be assigned to the subspecies G. m. letabae on geographic grounds and had a similar karyotype (2n = 52, FNa = 96) to those published for a panther genet from Ethiopia and for the West African large-spotted genet G. pardina. Notably, the specimen had a different autosomal morphology (2 acrocentric chromosomes) from that previously attributed to letabae (a single acrocentric chromosome), but the latter assignment was uncertain because the studied individuals were captive born and assigned based solely on a presumed origin in the former Transvaal Province of South Africa. Fluorescence in situ hybridization with a telomere repeat probe revealed the presence of telomeric sequences in the centromeres of most chromosomes, the so-called interstitial telomeric sites (ITSs). Since genets seem to have a unique, highly rearranged karyotype among feliforms and relatively low interspecific karyotypic variation, and considering the known instability of ITSs, we suggest that the large amount of ITSs found here might be due to evolutionarily recent extensive genomic rearrangements. This study provides cytogenetic information that contributes to our understanding of chromosomal variation and genomic rearrangements in genets, and valuable baseline data for future studies of karyotype evolution in carnivores in general and viverrids in particular.

© 2016 S. Karger AG, Basel



Related Articles:

References

  1. Adega F, Chaves R, Guedes-Pinto H: Chromosome restriction enzyme digestion in domestic pig (Sus scrofa) constitutive heterochromatin arrangement. Genes Genet Syst 80:49-56 (2005).
    External Resources
  2. Amadon D: The superspecies concept. Syst Biol 15:245-249 (1966).
    External Resources
  3. Baker RJ, Bickham JW: Speciation by monobrachial centric fusions. Proc Natl Acad Sci USA 83:8245-8248 (1986).
    External Resources
  4. Bertoni L, Attolini C, Faravelli M, Simi S, Giulotto E: Intrachromosomal telomere-like DNA sequences in Chinese hamster. Mamm Genome 7:853-855 (1996).
    External Resources
  5. Bruschi DP, Rivera M, Lima AP, Zúñiga AB, Recco-Pimentel SM: Interstitial telomeric sequences (ITS) and major rDNA mapping reveal insights into the karyotypical evolution of Neotropical leaf frogs species (Phyllomedusa, Hylidae, Anura). Mol Cytogenet 7:22 (2014).
    External Resources
  6. Chaves R, Adega F, Santos S, Guedes-Pinto H, Heslop-Harrinson JS: In situ hybridization and chromosome banding in mammalian species. Cytogenet Genome Res 96:113-116 (2002).
    External Resources
  7. Chaves R, Santos S, Guedes-Pinto H: Comparative analysis (Hippotragini versus Caprini, Bovidae) of X-chromosome's constitutive heterochromatin by in situ restriction endonuclease digestion: X-chromosome constitutive heterochromatin evolution. Genetica 121:315-325 (2004).
    External Resources
  8. Couturier J, Dutrillaux B: Évolution chromosomique chez les Carnivores. Mammalia 50:124-162 (1986).
    External Resources
  9. Crawford-Cabral J: Análise de dados craniométricos no género GenettaG. Cuvier (Carnivora, Viverridae). Memórias da Junta de Investigações Científicas do Ultramar 66:1-329 (1981).
  10. Crawford-Cabral J, Fernandes C: A comment on the nomenclature of the rusty-spotted genet. Small Carniv Conserv 21:12 (1999).
  11. Crawford-Cabral J, Fernandes CA: The rusty-spotted genets as a group with three species in Southern Africa (Carnivora. Viverridae), in Denys C, Granjon L, Poulet A (eds): African Small Mammals, pp 65-80 (I.R.D, Paris 2001).
  12. de la Seña C, Chowdhary BP, Gustavsson I: Localization of the telomeric (TTAGGG)n sequences in chromosomes of some domestic animals by fluorescence in situ hybridization. Hereditas 123:269-274 (1995).
    External Resources
  13. Fanning TG: Origin and evolution of a major feline satellite DNA. J Mol Biol 197:627-634 (1987).
    External Resources
  14. Fernandes CA, Ginja C, Pereira I, Tenreiro R, Bruford MW, Santos-Reis M: Species-specific mitochondrial DNA markers for identification of non-invasive samples from sympatric carnivores in the Iberian Peninsula. Conserv Genet 9:681-690 (2008).
    External Resources
  15. Finato AO, Varella-Garcia M, Tajara EH, Taddei VA, Morielle-Versute E: Intrachromosomal distribution of telomeric repeats in Eumops glaucinus and Euntops perotis (Molossidae, Chiroptera). Chromosome Res 8:563-569 (2000).
    External Resources
  16. Gaubert P, Fernandes CA, Bruford MW, Veron G: Genets (Carnivora, Viverridae) in Africa: an evolutionary synthesis based on cytochrome b sequences and morphological characters. Biol J Linn Soc 81:589-610 (2004a).
    External Resources
  17. Gaubert P, Aniskin VM, Dunham AE, Crémière C, Volobouev VT: Karyotype of the rare Johnston's genet Genetta johnstoni (Viverridae) and a reassessment of chromosomal characterization among congeneric species. Acta Theriol 49:457-464 (2004b).
    External Resources
  18. Gaubert P, Taylor PJ, Veron G: Integrative taxonomy and phylogenetic systematics of the genets (Carnivora, Viverridae, Genetta): a new classification of the most speciose carnivoran genus in Africa, in Huber BA, Sinclair BJ, Lampe KH (eds): African Biodiversity: Molecules, Organisms, Ecosystems, ed 1, pp 371-383 (Springer, New York 2005a).
    External Resources
  19. Gaubert P, Taylor PJ, Fernandes CA, Bruford MW, Veron G: Patterns of cryptic hybridization revealed using an integrative approach: a case study on genets (Carnivora, Viverridae, Genetta spp.) from the southern African subregion. Biol J Linn Soc 86:11-33 (2005b).
    External Resources
  20. Go Y, Rakotoarisoa G, Kawamoto Y, Randrianjafy A, Koyama N, Hirai H: PRINS analysis of the telomeric sequence in seven lemurs. Chromosome Res 8:57-65 (2000).
    External Resources
  21. Hanke M, Wink M: Direct DNA sequencing of PCR-amplified vector inserts following enzymatic degradation of primer and dNTPs. BioTechniques 17:858-860 (1994).
    External Resources
  22. Houck ML: Cytogenetic correlates of reproductive failure in species/subspecies crosses in zoos, in Lear T: Abstracts of the 13th North American Colloquium on Animal Cytogenetics and Gene Mapping, July 13-17, 2003, Louisville, Kentucky, USA. Cytogenet Genome Res 102:355-362 (2003).
    External Resources
  23. Ijdo JW, Wells RA, Baldini A, Reeders ST: Improved telomere detection using a telomere repeat probe (TTAGGG)n generated by PCR. Nucleic Acids Res 19:4780 (1991).
    External Resources
  24. Kingdon J, Hoffmann M: Mammals of Africa. Volume V: Carnivores, Pangolins, Equids and Rhinoceroses (Bloomsbury Publishing, London 2013).
  25. Kumamoto A: Large spotted genet (Genetta tigrina). House Report, pp 1-3 (San Diego Zoo, San Diego 1989).
  26. Li YC, Lee C, Sanoudou D, Hsu TH, Li SY, Lin CC: Interstitial colocalization of two cervid satellite DNAs involved in the genesis of the Indian muntjac karyotype. Chromosome Res 8:363-373 (2000).
    External Resources
  27. Liu WS, Fredga K: Telomeric (TTAGGG)n sequences are associated with nucleolus organizer regions (NORs) in the wood lemming. Chromosome Res 7:235-240 (1999).
    External Resources
  28. Mayr E: Animal Species and Evolution (Harvard University Press, Cambridge 1963).
  29. Meles S, Adega F, Guedes-Pinto H, Chaves R: The karyotype and sex chromosomes of Praomys tullbergi (Muridae, Rodentia): a detailed characterization. Micron 39:559-568 (2008).
    External Resources
  30. Metcalfe CJ, Eldridge MDB, Johnston PG: Mapping the distribution of the telomeric sequence (T2AG3)n in the Macropodoidea (Marsupialia) by fluorescence in situ hybridization. II. The ancestral 2n = 22 macropodid karyotype. Cytogenet Genome Res 116:212-217 (2007).
    External Resources
  31. Meyne J, Baker RJ, Hobart HH, Hsu TC, Ryder OA, et al: Distribution of non-telomeric sites of the (TTAGGG)n telomeric sequence in vertebrate chromosomes. Chromosoma 99:3-10 (1990).
    External Resources
  32. Morgulis A, Coulouris G, Raytselis Y, Madden TL, Agarwala R, Schäffer AA: Database indexing for production MegaBLAST searches. Bioinformatics 24:1757-1764 (2008).
    External Resources
  33. Munch K, Boomsma W, Huelsenbeck JP, Willerslev E, Nielsen R: Statistical assignment of DNA sequences using Bayesian phylogenetics. Syst Biol 57:750-757 (2008).
    External Resources
  34. Nie W, Wang J, Su W, Wang D, Tanomtong A, et al: Chromosomal rearrangements and karyotype evolution in carnivores revealed by chromosome painting. Heredity 108:17-27 (2012).
    External Resources
  35. Oom MM, Silva R, Kjöllerström J, Rampin M, Fernandes C, et al: Chromosome differentiation in the two non-African isolates of Genetta genetta, in: 19th International Colloquium on Animal Cytogenetics and Gene Mapping. Chromosome Res 18:711-755 (2010).
  36. Paço A, Chaves R, Vieira-da-Silva A, Adega F: The involvement of repetitive sequences in the remodelling of karyotypes: the Phodopus genomes (Rodentia, Cricetidae). Micron 46:27-34 (2013).
    External Resources
  37. Pathak S: Centromere or telomere: who is the boss? Anticancer Res 15:2549-2550 (1995).
    External Resources
  38. Perelman PL, Graphodatsky AS, Serdukova NA, Nie W, Alkalaeva EZ, et al: Karyotypic conservatism in the suborder Feliformia (order Carnivora). Cytogenet Genome Res 108:348-354 (2005).
    External Resources
  39. Perelman PL, Beklemisheva VR, Yudkin DV, Petrina TN, Rozhnov VV, et al: Comparative chromosome painting in Carnivora and Pholidota. Cytogenet Genome Res 137:174-193 (2012).
    External Resources
  40. Raudsepp T, Chowdhary BP: FISH for mapping single copy genes, in Murphy WJ (ed): Phylogenomics, Methods in Molecular Biology, pp 31-49 (Humana Press Inc., New Jersey 2008).
    External Resources
  41. Rogatcheva MB, Ono T, Sonta S, Oda S, Borodin PV: Robertsonian metacentrics of the house musk shrew (Suncus murinus, Insectivora, Soricidae) lose the telomeric sequences in the centromeric area. Genes Genet Syst 75:155-158 (2000).
    External Resources
  42. Rovatsos MT, Marchal JA, Romero-Fernández I, Fernández FJ, Giagia-Athanosopoulou EB, Sánchez A: Rapid, independent, and extensive amplification of telomeric repeats in pericentromeric regions in karyotypes of arvicoline rodents. Chromosome Res 19:869-882 (2011).
    External Resources
  43. Ruiz-Herrera A, Nergadze SG, Santagostino M, Giulotto E: Telomeric repeats far from the ends: mechanisms of origin and role in evolution. Cytogenet Genome Res 122:219-228 (2008).
    External Resources
  44. Santani A, Raudsepp T, Chowdhary BP: Interstitial telomeric sites and NORs in Hartmann's zebra (Equus zebra hartmannae) chromosomes. Chromosome Res 10:527-534 (2002).
    External Resources
  45. Santos S, Chaves R, Guedes-Pinto H: Chromosomal localization of the major satellite DNA family (FA-SAT) in the domestic cat. Cytogenet Genome Res 107:119-122 (2004).
    External Resources
  46. Schlawe L: Material, Fundorte, Text- und Bildquellen als Grundlagen für eine Artenliste zur Revision der Gattung Genetta G. CUVIER, 1816 (Mammalia, Carnivora, Viverridae). Zool Abh Staatl Mus Tierk Dresden 37:85-182 (1981).
  47. Slijepcevic P: Telomeres and mechanisms of Robertsonian fusion. Chromosoma 107:136-140 (1998).
    External Resources
  48. Sumner AT: A simple technique for demonstrating centromeric heterochromatin. Exp Cell Res 75:304-306 (1972).
    External Resources
  49. Swier VJ, Anwarali Khan FA, Baker RJ: Do time, heterochromatin, NORs, or chromosomal rearrangements correlate with distribution of interstitial telomeric repeats in Sigmodon (cotton rats)? J Hered 103:493-502 (2012).
    External Resources
  50. Ventura K, Silva MJ, Fagundes V, Christoff AU, Yonenaga-Yassuda Y: Non-telomeric sites as evidence of chromosomal rearrangement and repetitive (TTAGGG)n arrays in heterochromatic and euchromatic regions in four species of Akodon (Rodentia, Muridae). Cytogenet Genome Res 115:169-175 (2006).
    External Resources
  51. Vermeesch JR, De Meurichy W, Van Den Berghe H, Marynen P, Petit P: Differences in the distribution and nature of the interstitial telomeric (TTAGGG)n sequences in the chromosomes of the Giraffidae, okapai (Okapia johnstoni), and giraffe (Giraffa camelopardalis): evidence for ancestral telomeres at the okapi polymorphic rob(5;26) fusion site. Cytogenet Cell Genet 72:310-315 (1996).
    External Resources
  52. Wenzel E, Haltenorth T: System der Schleichkatzen (Viverridae). Säugetierkundliche Mitteilungen 20:110-127 (1972).
  53. Werle E, Schneider C, Renner M, Völker M, Fiehn W: Convenient single-step, one tube purification of PCR products for direct sequencing. Nucleic Acids Res 22:4354-4355 (1994).
    External Resources
  54. Wilson DE, Mittermeier RA: Handbook of the Mammals of the World, Volume 1, Carnivores (Lynx Editions, Barcelona 2009).
  55. Wurster DH, Benirschke K: Comparative cytogenetic studies in the order Carnivora. Chromosoma 24:336-382 (1968).
    External Resources
  56. Wurster-Hill DH, Gray CW: The interrelationships of chromosome banding patterns in procyonids, viverrids and felids. Cytogenet Cell Genet 15:306-331 (1975).
    External Resources
  57. Wurster-Hill DH, Ward OG, Davis BH, Park JP, Moyzis RK, Meyne J: Fragile sites, telomeric DNA sequences, B chromosomes, and DNA content in raccoon dogs, Nyctereutes procyonoides, with comparative notes on foxes, coyote, wolf, and raccoon. Cytogenet Cell Genet 49:278-281 (1988).
    External Resources
  58. Zhang Z, Schwartz S, Wagner L, Miller W: A greedy algorithm for aligning DNA sequences. J Comput Biol 7:203-214 (2000).
    External Resources

Article / Publication Details

First-Page Preview
Abstract of Original Article

Accepted: August 08, 2016
Published online: October 27, 2016
Issue release date: December 2016

Number of Print Pages: 8
Number of Figures: 3
Number of Tables: 0

ISSN: 1424-8581 (Print)
eISSN: 1424-859X (Online)

For additional information: https://www.karger.com/CGR

Copyright / Drug Dosage / Disclaimer

Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher.
Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

Article Tools
Article Details

2016, Vol.149, No. 4

December 2016

Article

Recommend This
TOP