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Molecules and Cells in Allergy - Original Paper

Serological Reactivity and Identification of Immunoglobulin E-Binding Polypeptides of Ganoderma applanatum Crude Spore Cytoplasmic Extract in Puerto Rican Subjects

Vilá-Héreter F.a · Rivera-Mariani F.E.b · Bolaños-Rosero B.a

Author affiliations

aDepartment of Microbiology, School of Medicine, University of Puerto Rico, Medical Sciences Campus, San Juan, PR, and bDepartment of Biology, Miami Dade College, Miami, FL, USA

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Int Arch Allergy Immunol 2017;172:139-149

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Article / Publication Details

First-Page Preview
Abstract of Molecules and Cells in Allergy - Original Paper

Received: June 23, 2016
Accepted: December 21, 2016
Published online: April 06, 2017
Issue release date: May 2017

Number of Print Pages: 11
Number of Figures: 3
Number of Tables: 5

ISSN: 1018-2438 (Print)
eISSN: 1423-0097 (Online)

For additional information: https://www.karger.com/IAA

Abstract

Background: The allergenic potential of Ganoderma applanatum basidiospores has been demonstrated previously in Puerto Rico. However, basidiomycete allergens are not available for inclusion in allergy diagnostic panels. Therefore, we sought to confirm allergic sensitization to G. applanatum crude spore cytoplasmic extract through reactivity in serological assays and detection of immunoglobulin E (IgE)-binding polypeptides. Methods: Via an indirect ELISA, serological reactivity was compared between groups of individuals with different allergic profiles. Group 1 (n = 51) consisted of individuals with sIgE to the allergens included in the diagnostic panels; group 2 (n = 14) comprised individuals with no sIgE to the allergens tested; and group 3 (n = 22) included individuals with no allergic history. To visualize IgE-binding polypeptides, group 1 sera were examined via Western blotting (WB). Polypeptide bands with the highest reactivity were analyzed by mass spectrometry (MS) for putative identification. Results: The serological reactivity of group 1 was significantly higher than that of group 3 in an indirect ELISA (p = 0.03). Sixty-five percent of group 1 individuals showed reactivity to polypeptide bands in WB. Bands of 81 and 56 kDa had the highest reactivity proportions among the reactive sera, followed by a 45-kDa band. MS analysis of these 3 polypeptides suggests that they are basidiomycete-derived enzymes with aconitate hydratase, catalase, and enolase functions. Conclusions:G. applanatum spores have allergenic components recognized by Puerto Rican individuals, which could eventually be considered as markers in cases of fungal allergy and be included in diagnostic allergen panels in Puerto Rico and tropical regions.

© 2017 S. Karger AG, Basel


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Article / Publication Details

First-Page Preview
Abstract of Molecules and Cells in Allergy - Original Paper

Received: June 23, 2016
Accepted: December 21, 2016
Published online: April 06, 2017
Issue release date: May 2017

Number of Print Pages: 11
Number of Figures: 3
Number of Tables: 5

ISSN: 1018-2438 (Print)
eISSN: 1423-0097 (Online)

For additional information: https://www.karger.com/IAA


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